Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26800 and RR42_RS26805 are separated by 257 nucleotides RR42_RS26805 and RR42_RS26810 are separated by 26 nucleotides RR42_RS26810 and RR42_RS26815 are separated by 139 nucleotides
RR42_RS26800: RR42_RS26800 - RND transporter, at 1,364,730 to 1,365,905
_RS26800
RR42_RS26805: RR42_RS26805 - class V aminotransferase, at 1,366,163 to 1,367,371
_RS26805
RR42_RS26810: RR42_RS26810 - endoribonuclease, at 1,367,398 to 1,367,871
_RS26810
RR42_RS26815: RR42_RS26815 - LysR family transcriptional regulator, at 1,368,011 to 1,368,928
_RS26815
Position (kb)
1366
1367
1368 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1365.178 kb on - strand, within RR42_RS26800 at 1365.219 kb on + strand, within RR42_RS26800 at 1365.220 kb on - strand, within RR42_RS26800 at 1365.223 kb on + strand, within RR42_RS26800 at 1365.223 kb on + strand, within RR42_RS26800 at 1365.223 kb on + strand, within RR42_RS26800 at 1365.224 kb on - strand, within RR42_RS26800 at 1365.363 kb on - strand, within RR42_RS26800 at 1365.830 kb on - strand at 1365.939 kb on - strand at 1366.016 kb on + strand at 1366.017 kb on - strand at 1366.017 kb on - strand at 1366.049 kb on + strand at 1366.050 kb on - strand at 1366.102 kb on + strand at 1366.224 kb on + strand at 1366.224 kb on + strand at 1366.224 kb on + strand at 1366.225 kb on - strand at 1366.284 kb on + strand, within RR42_RS26805 at 1366.285 kb on - strand, within RR42_RS26805 at 1366.285 kb on - strand, within RR42_RS26805 at 1366.285 kb on - strand, within RR42_RS26805 at 1366.617 kb on + strand, within RR42_RS26805 at 1366.617 kb on + strand, within RR42_RS26805 at 1366.618 kb on - strand, within RR42_RS26805 at 1366.618 kb on - strand, within RR42_RS26805 at 1366.618 kb on - strand, within RR42_RS26805 at 1366.668 kb on + strand, within RR42_RS26805 at 1366.669 kb on - strand, within RR42_RS26805 at 1366.669 kb on - strand, within RR42_RS26805 at 1366.669 kb on - strand, within RR42_RS26805 at 1366.939 kb on + strand at 1367.209 kb on + strand, within RR42_RS26805 at 1367.232 kb on + strand, within RR42_RS26805 at 1367.232 kb on + strand, within RR42_RS26805 at 1367.233 kb on - strand, within RR42_RS26805 at 1367.312 kb on - strand at 1367.565 kb on + strand, within RR42_RS26810 at 1367.566 kb on - strand, within RR42_RS26810 at 1367.697 kb on + strand, within RR42_RS26810 at 1367.760 kb on + strand, within RR42_RS26810 at 1367.761 kb on - strand, within RR42_RS26810 at 1367.762 kb on + strand, within RR42_RS26810 at 1367.762 kb on + strand, within RR42_RS26810 at 1367.763 kb on - strand, within RR42_RS26810 at 1367.763 kb on - strand, within RR42_RS26810 at 1367.763 kb on - strand, within RR42_RS26810 at 1367.791 kb on + strand, within RR42_RS26810 at 1367.791 kb on + strand, within RR42_RS26810 at 1367.795 kb on + strand, within RR42_RS26810 at 1367.795 kb on + strand, within RR42_RS26810 at 1367.795 kb on + strand, within RR42_RS26810 at 1367.795 kb on + strand, within RR42_RS26810 at 1367.796 kb on - strand, within RR42_RS26810 at 1367.796 kb on - strand, within RR42_RS26810 at 1367.796 kb on - strand, within RR42_RS26810 at 1367.796 kb on - strand, within RR42_RS26810 at 1367.974 kb on + strand at 1367.975 kb on - strand at 1368.249 kb on + strand, within RR42_RS26815 at 1368.249 kb on + strand, within RR42_RS26815 at 1368.249 kb on + strand, within RR42_RS26815 at 1368.250 kb on - strand, within RR42_RS26815
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,365,178 - RR42_RS26800 0.38 -0.6 1,365,219 + RR42_RS26800 0.42 -0.7 1,365,220 - RR42_RS26800 0.42 -0.1 1,365,223 + RR42_RS26800 0.42 +0.4 1,365,223 + RR42_RS26800 0.42 -2.3 1,365,223 + RR42_RS26800 0.42 +1.4 1,365,224 - RR42_RS26800 0.42 +0.8 1,365,363 - RR42_RS26800 0.54 -0.3 1,365,830 - +0.6 1,365,939 - -1.0 1,366,016 + -0.0 1,366,017 - +0.2 1,366,017 - +0.4 1,366,049 + +0.3 1,366,050 - +0.5 1,366,102 + +0.1 1,366,224 + +0.2 1,366,224 + +0.6 1,366,224 + -0.4 1,366,225 - -0.7 1,366,284 + RR42_RS26805 0.10 -0.4 1,366,285 - RR42_RS26805 0.10 -1.6 1,366,285 - RR42_RS26805 0.10 -1.5 1,366,285 - RR42_RS26805 0.10 -2.1 1,366,617 + RR42_RS26805 0.38 -1.5 1,366,617 + RR42_RS26805 0.38 +0.8 1,366,618 - RR42_RS26805 0.38 -0.4 1,366,618 - RR42_RS26805 0.38 -0.0 1,366,618 - RR42_RS26805 0.38 -0.3 1,366,668 + RR42_RS26805 0.42 -0.2 1,366,669 - RR42_RS26805 0.42 -0.2 1,366,669 - RR42_RS26805 0.42 -0.3 1,366,669 - RR42_RS26805 0.42 -0.2 1,366,939 + +2.4 1,367,209 + RR42_RS26805 0.87 -0.2 1,367,232 + RR42_RS26805 0.88 -0.4 1,367,232 + RR42_RS26805 0.88 +0.0 1,367,233 - RR42_RS26805 0.89 -0.1 1,367,312 - +0.2 1,367,565 + RR42_RS26810 0.35 +0.9 1,367,566 - RR42_RS26810 0.35 -0.1 1,367,697 + RR42_RS26810 0.63 -0.6 1,367,760 + RR42_RS26810 0.76 -0.6 1,367,761 - RR42_RS26810 0.77 +0.1 1,367,762 + RR42_RS26810 0.77 +0.4 1,367,762 + RR42_RS26810 0.77 +0.2 1,367,763 - RR42_RS26810 0.77 +0.3 1,367,763 - RR42_RS26810 0.77 -0.7 1,367,763 - RR42_RS26810 0.77 -0.3 1,367,791 + RR42_RS26810 0.83 -0.3 1,367,791 + RR42_RS26810 0.83 -0.2 1,367,795 + RR42_RS26810 0.84 +1.4 1,367,795 + RR42_RS26810 0.84 -0.2 1,367,795 + RR42_RS26810 0.84 -0.1 1,367,795 + RR42_RS26810 0.84 -0.6 1,367,796 - RR42_RS26810 0.84 +0.7 1,367,796 - RR42_RS26810 0.84 +0.6 1,367,796 - RR42_RS26810 0.84 -0.9 1,367,796 - RR42_RS26810 0.84 -0.1 1,367,974 + -0.7 1,367,975 - -0.4 1,368,249 + RR42_RS26815 0.26 +1.6 1,368,249 + RR42_RS26815 0.26 -0.2 1,368,249 + RR42_RS26815 0.26 +0.6 1,368,250 - RR42_RS26815 0.26 +0.3
Or see this region's nucleotide sequence