Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26400 and RR42_RS26405 are separated by 78 nucleotides RR42_RS26405 and RR42_RS26410 overlap by 4 nucleotides RR42_RS26410 and RR42_RS26415 are separated by 74 nucleotides RR42_RS26415 and RR42_RS26420 are separated by 43 nucleotides
RR42_RS26400: RR42_RS26400 - structural protein MipA, at 1,281,123 to 1,281,866
_RS26400
RR42_RS26405: RR42_RS26405 - hypothetical protein, at 1,281,945 to 1,282,661
_RS26405
RR42_RS26410: RR42_RS26410 - hypothetical protein, at 1,282,658 to 1,283,863
_RS26410
RR42_RS26415: RR42_RS26415 - MarR family transcriptional regulator, at 1,283,938 to 1,284,468
_RS26415
RR42_RS26420: RR42_RS26420 - dioxygenase, at 1,284,512 to 1,285,027
_RS26420
Position (kb) 1282
1283
1284 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1281.856 kb on - strand at 1281.881 kb on + strand at 1281.948 kb on + strand at 1282.197 kb on - strand, within RR42_RS26405 at 1282.203 kb on + strand, within RR42_RS26405 at 1282.453 kb on - strand, within RR42_RS26405 at 1282.626 kb on + strand at 1282.626 kb on + strand at 1282.651 kb on + strand at 1282.651 kb on + strand at 1282.914 kb on - strand, within RR42_RS26410 at 1282.965 kb on - strand, within RR42_RS26410 at 1283.063 kb on + strand, within RR42_RS26410 at 1283.163 kb on - strand, within RR42_RS26410 at 1283.163 kb on - strand, within RR42_RS26410 at 1283.163 kb on - strand, within RR42_RS26410 at 1283.304 kb on - strand, within RR42_RS26410 at 1283.771 kb on + strand at 1284.440 kb on - strand at 1284.699 kb on + strand, within RR42_RS26420 at 1284.748 kb on - strand, within RR42_RS26420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,281,856 - -5.0 1,281,881 + -1.8 1,281,948 + -0.5 1,282,197 - RR42_RS26405 0.35 -1.9 1,282,203 + RR42_RS26405 0.36 -1.8 1,282,453 - RR42_RS26405 0.71 +0.1 1,282,626 + -3.3 1,282,626 + -2.4 1,282,651 + -2.1 1,282,651 + -1.1 1,282,914 - RR42_RS26410 0.21 -2.4 1,282,965 - RR42_RS26410 0.25 -3.8 1,283,063 + RR42_RS26410 0.34 -3.6 1,283,163 - RR42_RS26410 0.42 -3.3 1,283,163 - RR42_RS26410 0.42 -3.5 1,283,163 - RR42_RS26410 0.42 -2.2 1,283,304 - RR42_RS26410 0.54 -1.8 1,283,771 + -1.6 1,284,440 - -0.9 1,284,699 + RR42_RS26420 0.36 +0.1 1,284,748 - RR42_RS26420 0.46 -0.7
Or see this region's nucleotide sequence