Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25970 and RR42_RS25975 are separated by 94 nucleotides RR42_RS25975 and RR42_RS25980 are separated by 220 nucleotides RR42_RS25980 and RR42_RS25985 are separated by 90 nucleotides
RR42_RS25970: RR42_RS25970 - acyl-CoA dehydrogenase, at 1,171,704 to 1,172,861
_RS25970
RR42_RS25975: RR42_RS25975 - hypothetical protein, at 1,172,956 to 1,173,195
_RS25975
RR42_RS25980: RR42_RS25980 - hypothetical protein, at 1,173,416 to 1,174,057
_RS25980
RR42_RS25985: RR42_RS25985 - cytochrome, at 1,174,148 to 1,174,522
_RS25985
Position (kb)
1172
1173
1174 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1172.003 kb on + strand, within RR42_RS25970 at 1172.004 kb on - strand, within RR42_RS25970 at 1172.196 kb on - strand, within RR42_RS25970 at 1172.324 kb on + strand, within RR42_RS25970 at 1172.325 kb on - strand, within RR42_RS25970 at 1172.508 kb on + strand, within RR42_RS25970 at 1172.683 kb on - strand, within RR42_RS25970 at 1172.683 kb on - strand, within RR42_RS25970 at 1172.802 kb on + strand at 1172.802 kb on + strand at 1172.802 kb on + strand at 1172.803 kb on - strand at 1172.803 kb on - strand at 1172.939 kb on + strand at 1172.939 kb on + strand at 1172.940 kb on - strand at 1172.947 kb on - strand at 1173.263 kb on + strand at 1173.268 kb on + strand at 1173.269 kb on - strand at 1173.362 kb on + strand at 1173.362 kb on + strand at 1173.363 kb on - strand at 1173.363 kb on - strand at 1173.369 kb on + strand at 1173.369 kb on + strand at 1173.370 kb on - strand at 1173.437 kb on + strand at 1173.528 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.655 kb on + strand, within RR42_RS25980 at 1173.912 kb on - strand at 1173.912 kb on - strand, within RR42_RS25980 at 1173.912 kb on - strand, within RR42_RS25980 at 1173.947 kb on + strand, within RR42_RS25980 at 1173.947 kb on + strand, within RR42_RS25980 at 1174.149 kb on + strand at 1174.149 kb on + strand at 1174.149 kb on + strand at 1174.150 kb on - strand at 1174.150 kb on - strand at 1174.181 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,172,003 + RR42_RS25970 0.26 -0.1 1,172,004 - RR42_RS25970 0.26 -1.9 1,172,196 - RR42_RS25970 0.42 +0.7 1,172,324 + RR42_RS25970 0.54 -1.4 1,172,325 - RR42_RS25970 0.54 -1.1 1,172,508 + RR42_RS25970 0.69 -0.8 1,172,683 - RR42_RS25970 0.85 -1.3 1,172,683 - RR42_RS25970 0.85 -1.6 1,172,802 + +0.2 1,172,802 + -2.1 1,172,802 + +0.5 1,172,803 - +0.1 1,172,803 - -0.1 1,172,939 + -1.0 1,172,939 + -1.3 1,172,940 - +2.9 1,172,947 - +0.3 1,173,263 + -0.7 1,173,268 + +0.8 1,173,269 - +0.7 1,173,362 + -0.1 1,173,362 + -0.0 1,173,363 - -1.0 1,173,363 - +0.0 1,173,369 + +0.3 1,173,369 + +1.2 1,173,370 - -0.1 1,173,437 + +0.2 1,173,528 + RR42_RS25980 0.17 -0.9 1,173,655 + RR42_RS25980 0.37 -0.6 1,173,655 + RR42_RS25980 0.37 +0.1 1,173,655 + RR42_RS25980 0.37 +1.0 1,173,655 + RR42_RS25980 0.37 -0.6 1,173,912 - -0.3 1,173,912 - RR42_RS25980 0.77 +0.4 1,173,912 - RR42_RS25980 0.77 +0.6 1,173,947 + RR42_RS25980 0.83 +0.4 1,173,947 + RR42_RS25980 0.83 +0.0 1,174,149 + -0.6 1,174,149 + +0.7 1,174,149 + -0.0 1,174,150 - +0.0 1,174,150 - +0.6 1,174,181 - -0.4
Or see this region's nucleotide sequence