Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25825 and RR42_RS25830 are separated by 67 nucleotides RR42_RS25830 and RR42_RS25835 overlap by 4 nucleotides
RR42_RS25825: RR42_RS25825 - histidine kinase, at 1,132,837 to 1,133,787
_RS25825
RR42_RS25830: RR42_RS25830 - cytochrome BD ubiquinol oxidase subunit II, at 1,133,855 to 1,134,877
_RS25830
RR42_RS25835: RR42_RS25835 - cytochrome D ubiquinol oxidase subunit I, at 1,134,874 to 1,136,253
_RS25835
Position (kb)
1133
1134
1135 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1133.135 kb on + strand, within RR42_RS25825 at 1133.210 kb on + strand, within RR42_RS25825 at 1133.213 kb on + strand, within RR42_RS25825 at 1133.249 kb on + strand, within RR42_RS25825 at 1133.250 kb on - strand, within RR42_RS25825 at 1133.259 kb on - strand, within RR42_RS25825 at 1133.418 kb on - strand, within RR42_RS25825 at 1133.636 kb on + strand, within RR42_RS25825 at 1133.637 kb on - strand, within RR42_RS25825 at 1133.787 kb on + strand at 1133.807 kb on + strand at 1133.807 kb on + strand at 1133.845 kb on + strand at 1133.846 kb on + strand at 1133.846 kb on + strand at 1133.846 kb on + strand at 1133.846 kb on + strand at 1133.846 kb on + strand at 1133.847 kb on - strand at 1133.847 kb on - strand at 1133.847 kb on - strand at 1133.850 kb on + strand at 1133.850 kb on + strand at 1133.850 kb on + strand at 1133.862 kb on + strand at 1133.891 kb on + strand at 1133.891 kb on + strand at 1133.892 kb on - strand at 1133.899 kb on - strand at 1133.905 kb on - strand at 1133.914 kb on - strand at 1133.949 kb on - strand at 1133.996 kb on - strand, within RR42_RS25830 at 1134.313 kb on - strand, within RR42_RS25830 at 1134.313 kb on - strand, within RR42_RS25830 at 1134.378 kb on + strand, within RR42_RS25830 at 1134.378 kb on + strand, within RR42_RS25830 at 1134.451 kb on + strand, within RR42_RS25830 at 1134.452 kb on - strand, within RR42_RS25830 at 1134.453 kb on + strand, within RR42_RS25830 at 1134.453 kb on + strand, within RR42_RS25830 at 1134.454 kb on - strand, within RR42_RS25830 at 1134.454 kb on - strand, within RR42_RS25830 at 1134.454 kb on - strand, within RR42_RS25830 at 1134.454 kb on - strand, within RR42_RS25830 at 1134.454 kb on - strand, within RR42_RS25830 at 1134.801 kb on + strand at 1134.802 kb on - strand at 1134.802 kb on - strand at 1134.989 kb on + strand at 1134.990 kb on - strand at 1135.010 kb on + strand at 1135.010 kb on + strand at 1135.011 kb on - strand at 1135.011 kb on - strand at 1135.011 kb on - strand at 1135.023 kb on - strand, within RR42_RS25835 at 1135.023 kb on - strand, within RR42_RS25835 at 1135.023 kb on - strand, within RR42_RS25835 at 1135.095 kb on - strand, within RR42_RS25835 at 1135.104 kb on - strand, within RR42_RS25835 at 1135.104 kb on - strand, within RR42_RS25835 at 1135.130 kb on + strand, within RR42_RS25835 at 1135.130 kb on + strand, within RR42_RS25835 at 1135.181 kb on - strand, within RR42_RS25835 at 1135.371 kb on - strand, within RR42_RS25835 at 1135.692 kb on + strand, within RR42_RS25835 at 1135.693 kb on - strand, within RR42_RS25835 at 1135.693 kb on - strand, within RR42_RS25835 at 1135.693 kb on - strand, within RR42_RS25835 at 1135.693 kb on - strand, within RR42_RS25835 at 1135.866 kb on + strand, within RR42_RS25835 at 1135.867 kb on - strand, within RR42_RS25835
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,133,135 + RR42_RS25825 0.31 +0.2 1,133,210 + RR42_RS25825 0.39 +0.2 1,133,213 + RR42_RS25825 0.40 -0.4 1,133,249 + RR42_RS25825 0.43 -1.4 1,133,250 - RR42_RS25825 0.43 +0.2 1,133,259 - RR42_RS25825 0.44 -0.7 1,133,418 - RR42_RS25825 0.61 +0.2 1,133,636 + RR42_RS25825 0.84 -0.6 1,133,637 - RR42_RS25825 0.84 -1.4 1,133,787 + -1.4 1,133,807 + +0.9 1,133,807 + +0.4 1,133,845 + -0.3 1,133,846 + -0.1 1,133,846 + +1.2 1,133,846 + +0.3 1,133,846 + +0.1 1,133,846 + -1.8 1,133,847 - -0.1 1,133,847 - -0.0 1,133,847 - +0.3 1,133,850 + +1.1 1,133,850 + +0.5 1,133,850 + +0.1 1,133,862 + -0.9 1,133,891 + -0.6 1,133,891 + +0.9 1,133,892 - -0.2 1,133,899 - -0.7 1,133,905 - -0.8 1,133,914 - +0.3 1,133,949 - +0.3 1,133,996 - RR42_RS25830 0.14 +0.6 1,134,313 - RR42_RS25830 0.45 -0.4 1,134,313 - RR42_RS25830 0.45 +2.0 1,134,378 + RR42_RS25830 0.51 +0.1 1,134,378 + RR42_RS25830 0.51 -0.3 1,134,451 + RR42_RS25830 0.58 +0.1 1,134,452 - RR42_RS25830 0.58 -0.2 1,134,453 + RR42_RS25830 0.58 +0.3 1,134,453 + RR42_RS25830 0.58 -0.2 1,134,454 - RR42_RS25830 0.59 -1.4 1,134,454 - RR42_RS25830 0.59 -0.1 1,134,454 - RR42_RS25830 0.59 -0.7 1,134,454 - RR42_RS25830 0.59 -0.4 1,134,454 - RR42_RS25830 0.59 +0.5 1,134,801 + +0.7 1,134,802 - -1.8 1,134,802 - -0.1 1,134,989 + +0.3 1,134,990 - -1.9 1,135,010 + -0.2 1,135,010 + -1.1 1,135,011 - +1.8 1,135,011 - +0.3 1,135,011 - -0.1 1,135,023 - RR42_RS25835 0.11 +1.0 1,135,023 - RR42_RS25835 0.11 -1.7 1,135,023 - RR42_RS25835 0.11 -0.2 1,135,095 - RR42_RS25835 0.16 -0.2 1,135,104 - RR42_RS25835 0.17 +0.6 1,135,104 - RR42_RS25835 0.17 +0.2 1,135,130 + RR42_RS25835 0.19 -1.4 1,135,130 + RR42_RS25835 0.19 +1.5 1,135,181 - RR42_RS25835 0.22 +1.7 1,135,371 - RR42_RS25835 0.36 +1.9 1,135,692 + RR42_RS25835 0.59 +0.7 1,135,693 - RR42_RS25835 0.59 +0.9 1,135,693 - RR42_RS25835 0.59 +0.1 1,135,693 - RR42_RS25835 0.59 +0.3 1,135,693 - RR42_RS25835 0.59 -0.6 1,135,866 + RR42_RS25835 0.72 +1.3 1,135,867 - RR42_RS25835 0.72 +1.8
Or see this region's nucleotide sequence