Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25570 and RR42_RS25575 are separated by 45 nucleotides RR42_RS25575 and RR42_RS25580 are separated by 79 nucleotides RR42_RS25580 and RR42_RS25585 are separated by 237 nucleotides
RR42_RS25570: RR42_RS25570 - long-chain fatty acid--CoA ligase, at 1,080,721 to 1,082,400
_RS25570
RR42_RS25575: RR42_RS25575 - aldolase, at 1,082,446 to 1,083,201
_RS25575
RR42_RS25580: RR42_RS25580 - haloacid dehalogenase, at 1,083,281 to 1,083,991
_RS25580
RR42_RS25585: RR42_RS25585 - membrane protein, at 1,084,229 to 1,085,392
_RS25585
Position (kb)
1083
1084 Strain fitness (log2 ratio)
-1
0
1 at 1082.429 kb on - strand at 1082.429 kb on - strand at 1082.433 kb on - strand at 1082.449 kb on + strand at 1082.449 kb on + strand at 1082.449 kb on + strand at 1082.450 kb on - strand at 1082.450 kb on - strand at 1082.778 kb on + strand, within RR42_RS25575 at 1082.847 kb on - strand, within RR42_RS25575 at 1082.847 kb on - strand, within RR42_RS25575 at 1082.867 kb on - strand, within RR42_RS25575 at 1082.875 kb on + strand, within RR42_RS25575 at 1082.876 kb on - strand, within RR42_RS25575 at 1082.876 kb on - strand, within RR42_RS25575 at 1082.883 kb on + strand, within RR42_RS25575 at 1082.883 kb on + strand, within RR42_RS25575 at 1082.942 kb on - strand, within RR42_RS25575 at 1082.976 kb on - strand, within RR42_RS25575 at 1082.976 kb on - strand, within RR42_RS25575 at 1082.976 kb on - strand, within RR42_RS25575 at 1083.010 kb on + strand, within RR42_RS25575 at 1083.010 kb on + strand, within RR42_RS25575 at 1083.011 kb on - strand, within RR42_RS25575 at 1083.177 kb on + strand at 1083.328 kb on - strand at 1083.350 kb on + strand at 1083.350 kb on + strand at 1083.351 kb on - strand at 1083.431 kb on + strand, within RR42_RS25580 at 1083.432 kb on - strand, within RR42_RS25580 at 1083.432 kb on - strand, within RR42_RS25580 at 1083.668 kb on - strand, within RR42_RS25580 at 1083.705 kb on + strand, within RR42_RS25580 at 1083.746 kb on + strand, within RR42_RS25580 at 1083.746 kb on + strand, within RR42_RS25580 at 1083.747 kb on - strand, within RR42_RS25580 at 1083.875 kb on + strand, within RR42_RS25580 at 1083.875 kb on + strand, within RR42_RS25580 at 1083.876 kb on - strand, within RR42_RS25580 at 1083.876 kb on - strand, within RR42_RS25580 at 1084.116 kb on + strand at 1084.116 kb on + strand at 1084.117 kb on - strand at 1084.131 kb on + strand at 1084.199 kb on + strand at 1084.307 kb on + strand at 1084.308 kb on - strand at 1084.335 kb on - strand at 1084.453 kb on + strand, within RR42_RS25585 at 1084.453 kb on + strand, within RR42_RS25585 at 1084.728 kb on - strand, within RR42_RS25585 at 1084.750 kb on + strand, within RR42_RS25585 at 1084.887 kb on - strand, within RR42_RS25585 at 1084.920 kb on - strand, within RR42_RS25585 at 1084.920 kb on - strand, within RR42_RS25585 at 1084.989 kb on - strand, within RR42_RS25585 at 1084.989 kb on - strand, within RR42_RS25585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,082,429 - +1.1 1,082,429 - +0.4 1,082,433 - +0.8 1,082,449 + -0.1 1,082,449 + +0.1 1,082,449 + +1.2 1,082,450 - +0.6 1,082,450 - -0.5 1,082,778 + RR42_RS25575 0.44 -0.7 1,082,847 - RR42_RS25575 0.53 +0.2 1,082,847 - RR42_RS25575 0.53 +0.2 1,082,867 - RR42_RS25575 0.56 -0.3 1,082,875 + RR42_RS25575 0.57 +0.1 1,082,876 - RR42_RS25575 0.57 +0.0 1,082,876 - RR42_RS25575 0.57 -0.4 1,082,883 + RR42_RS25575 0.58 -0.5 1,082,883 + RR42_RS25575 0.58 -0.2 1,082,942 - RR42_RS25575 0.66 -0.6 1,082,976 - RR42_RS25575 0.70 +0.3 1,082,976 - RR42_RS25575 0.70 -0.4 1,082,976 - RR42_RS25575 0.70 -0.4 1,083,010 + RR42_RS25575 0.75 -0.3 1,083,010 + RR42_RS25575 0.75 +0.2 1,083,011 - RR42_RS25575 0.75 -0.6 1,083,177 + +0.1 1,083,328 - -0.6 1,083,350 + +0.1 1,083,350 + -0.6 1,083,351 - -0.1 1,083,431 + RR42_RS25580 0.21 -0.2 1,083,432 - RR42_RS25580 0.21 -0.6 1,083,432 - RR42_RS25580 0.21 -1.4 1,083,668 - RR42_RS25580 0.54 +0.8 1,083,705 + RR42_RS25580 0.60 +0.0 1,083,746 + RR42_RS25580 0.65 -0.5 1,083,746 + RR42_RS25580 0.65 -0.9 1,083,747 - RR42_RS25580 0.66 -0.1 1,083,875 + RR42_RS25580 0.84 -0.5 1,083,875 + RR42_RS25580 0.84 -0.1 1,083,876 - RR42_RS25580 0.84 +0.2 1,083,876 - RR42_RS25580 0.84 +0.1 1,084,116 + -1.0 1,084,116 + +0.5 1,084,117 - +1.3 1,084,131 + -0.7 1,084,199 + +0.2 1,084,307 + -0.2 1,084,308 - -0.5 1,084,335 - -0.3 1,084,453 + RR42_RS25585 0.19 +0.5 1,084,453 + RR42_RS25585 0.19 +0.6 1,084,728 - RR42_RS25585 0.43 -0.2 1,084,750 + RR42_RS25585 0.45 +0.1 1,084,887 - RR42_RS25585 0.57 -0.2 1,084,920 - RR42_RS25585 0.59 +0.6 1,084,920 - RR42_RS25585 0.59 +0.2 1,084,989 - RR42_RS25585 0.65 +0.4 1,084,989 - RR42_RS25585 0.65 +0.4
Or see this region's nucleotide sequence