Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23895 and RR42_RS23900 are separated by 10 nucleotides RR42_RS23900 and RR42_RS23905 are separated by 94 nucleotides RR42_RS23905 and RR42_RS23910 overlap by 4 nucleotides RR42_RS23910 and RR42_RS23915 are separated by 50 nucleotides RR42_RS23915 and RR42_RS23920 overlap by 1 nucleotides
RR42_RS23895: RR42_RS23895 - hypothetical protein, at 687,903 to 688,616
_RS23895
RR42_RS23900: RR42_RS23900 - hypothetical protein, at 688,627 to 689,109
_RS23900
RR42_RS23905: RR42_RS23905 - hypothetical protein, at 689,204 to 691,897
_RS23905
RR42_RS23910: RR42_RS23910 - hypothetical protein, at 691,894 to 692,343
_RS23910
RR42_RS23915: RR42_RS23915 - hypothetical protein, at 692,394 to 692,870
_RS23915
RR42_RS23920: RR42_RS23920 - hypothetical protein, at 692,870 to 693,496
_RS23920
Position (kb) 689
690
691
692 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 688.243 kb on - strand, within RR42_RS23895 at 688.243 kb on - strand, within RR42_RS23895 at 688.243 kb on - strand, within RR42_RS23895 at 688.320 kb on + strand, within RR42_RS23895 at 688.320 kb on + strand, within RR42_RS23895 at 688.626 kb on + strand at 688.627 kb on - strand at 688.765 kb on + strand, within RR42_RS23900 at 688.766 kb on - strand, within RR42_RS23900 at 688.886 kb on - strand, within RR42_RS23900 at 688.886 kb on - strand, within RR42_RS23900 at 688.978 kb on + strand, within RR42_RS23900 at 688.979 kb on - strand, within RR42_RS23900 at 688.984 kb on + strand, within RR42_RS23900 at 688.985 kb on - strand, within RR42_RS23900 at 688.985 kb on - strand, within RR42_RS23900 at 688.985 kb on - strand, within RR42_RS23900 at 688.985 kb on - strand, within RR42_RS23900 at 688.985 kb on - strand, within RR42_RS23900 at 688.999 kb on - strand, within RR42_RS23900 at 689.240 kb on + strand at 689.322 kb on - strand at 689.789 kb on - strand, within RR42_RS23905 at 689.789 kb on - strand, within RR42_RS23905 at 689.789 kb on - strand, within RR42_RS23905 at 689.805 kb on - strand, within RR42_RS23905 at 689.810 kb on + strand, within RR42_RS23905 at 690.038 kb on + strand, within RR42_RS23905 at 690.039 kb on - strand, within RR42_RS23905 at 690.089 kb on - strand, within RR42_RS23905 at 690.089 kb on - strand, within RR42_RS23905 at 690.089 kb on - strand, within RR42_RS23905 at 690.325 kb on - strand, within RR42_RS23905 at 690.458 kb on + strand, within RR42_RS23905 at 690.644 kb on - strand, within RR42_RS23905 at 690.668 kb on + strand, within RR42_RS23905 at 690.668 kb on + strand, within RR42_RS23905 at 690.668 kb on + strand, within RR42_RS23905 at 690.668 kb on + strand, within RR42_RS23905 at 690.668 kb on + strand, within RR42_RS23905 at 690.668 kb on + strand, within RR42_RS23905 at 690.669 kb on - strand, within RR42_RS23905 at 690.669 kb on - strand, within RR42_RS23905 at 690.669 kb on - strand, within RR42_RS23905 at 690.818 kb on + strand, within RR42_RS23905 at 690.819 kb on - strand, within RR42_RS23905 at 691.185 kb on - strand, within RR42_RS23905 at 691.257 kb on + strand, within RR42_RS23905 at 691.325 kb on + strand, within RR42_RS23905 at 691.326 kb on - strand, within RR42_RS23905 at 691.326 kb on - strand, within RR42_RS23905 at 691.440 kb on - strand, within RR42_RS23905 at 691.484 kb on + strand, within RR42_RS23905 at 691.484 kb on + strand, within RR42_RS23905 at 691.485 kb on - strand, within RR42_RS23905 at 691.601 kb on + strand, within RR42_RS23905 at 691.602 kb on - strand, within RR42_RS23905 at 691.602 kb on - strand, within RR42_RS23905 at 691.602 kb on - strand, within RR42_RS23905 at 691.844 kb on + strand at 691.845 kb on - strand at 691.877 kb on + strand at 691.878 kb on - strand at 691.878 kb on - strand at 692.189 kb on + strand, within RR42_RS23910 at 692.189 kb on + strand, within RR42_RS23910 at 692.218 kb on + strand, within RR42_RS23910 at 692.453 kb on - strand, within RR42_RS23915 at 692.453 kb on - strand, within RR42_RS23915 at 692.668 kb on - strand, within RR42_RS23915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 688,243 - RR42_RS23895 0.48 +0.7 688,243 - RR42_RS23895 0.48 +1.1 688,243 - RR42_RS23895 0.48 -0.7 688,320 + RR42_RS23895 0.58 -1.3 688,320 + RR42_RS23895 0.58 +0.4 688,626 + +0.6 688,627 - -0.3 688,765 + RR42_RS23900 0.29 +0.6 688,766 - RR42_RS23900 0.29 +0.8 688,886 - RR42_RS23900 0.54 +0.3 688,886 - RR42_RS23900 0.54 +1.2 688,978 + RR42_RS23900 0.73 -0.7 688,979 - RR42_RS23900 0.73 +0.4 688,984 + RR42_RS23900 0.74 +0.4 688,985 - RR42_RS23900 0.74 -0.5 688,985 - RR42_RS23900 0.74 +0.2 688,985 - RR42_RS23900 0.74 +0.5 688,985 - RR42_RS23900 0.74 -1.6 688,985 - RR42_RS23900 0.74 -0.1 688,999 - RR42_RS23900 0.77 -3.3 689,240 + -2.6 689,322 - +0.2 689,789 - RR42_RS23905 0.22 +0.1 689,789 - RR42_RS23905 0.22 -0.8 689,789 - RR42_RS23905 0.22 -0.1 689,805 - RR42_RS23905 0.22 -0.5 689,810 + RR42_RS23905 0.22 -0.2 690,038 + RR42_RS23905 0.31 +1.4 690,039 - RR42_RS23905 0.31 +0.8 690,089 - RR42_RS23905 0.33 +0.4 690,089 - RR42_RS23905 0.33 +0.2 690,089 - RR42_RS23905 0.33 +0.0 690,325 - RR42_RS23905 0.42 -0.3 690,458 + RR42_RS23905 0.47 -1.1 690,644 - RR42_RS23905 0.53 +0.3 690,668 + RR42_RS23905 0.54 -0.0 690,668 + RR42_RS23905 0.54 -0.2 690,668 + RR42_RS23905 0.54 -0.3 690,668 + RR42_RS23905 0.54 +0.1 690,668 + RR42_RS23905 0.54 -0.1 690,668 + RR42_RS23905 0.54 -0.6 690,669 - RR42_RS23905 0.54 -0.4 690,669 - RR42_RS23905 0.54 +0.2 690,669 - RR42_RS23905 0.54 -0.1 690,818 + RR42_RS23905 0.60 +0.3 690,819 - RR42_RS23905 0.60 +0.2 691,185 - RR42_RS23905 0.74 -1.2 691,257 + RR42_RS23905 0.76 +0.3 691,325 + RR42_RS23905 0.79 -1.1 691,326 - RR42_RS23905 0.79 +0.7 691,326 - RR42_RS23905 0.79 -0.1 691,440 - RR42_RS23905 0.83 -0.2 691,484 + RR42_RS23905 0.85 -0.6 691,484 + RR42_RS23905 0.85 +0.2 691,485 - RR42_RS23905 0.85 -0.0 691,601 + RR42_RS23905 0.89 +2.4 691,602 - RR42_RS23905 0.89 -0.6 691,602 - RR42_RS23905 0.89 +0.5 691,602 - RR42_RS23905 0.89 -0.4 691,844 + -0.5 691,845 - -0.6 691,877 + -0.3 691,878 - +0.6 691,878 - +0.3 692,189 + RR42_RS23910 0.66 +0.6 692,189 + RR42_RS23910 0.66 +1.9 692,218 + RR42_RS23910 0.72 -0.0 692,453 - RR42_RS23915 0.12 -0.3 692,453 - RR42_RS23915 0.12 +0.1 692,668 - RR42_RS23915 0.57 +0.7
Or see this region's nucleotide sequence