Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23720 and RR42_RS23725 are separated by 270 nucleotides RR42_RS23725 and RR42_RS23730 are separated by 13 nucleotides RR42_RS23730 and RR42_RS23735 are separated by 15 nucleotides
RR42_RS23720: RR42_RS23720 - hypothetical protein, at 647,414 to 648,286
_RS23720
RR42_RS23725: RR42_RS23725 - phenylacetate-CoA oxygenase, at 648,557 to 649,561
_RS23725
RR42_RS23730: RR42_RS23730 - phenylacetate-CoA oxygenase subunit PaaB, at 649,575 to 649,859
_RS23730
RR42_RS23735: RR42_RS23735 - phenylacetyl-CoA 1,2-epoxidase, structural subunit (EC 1.14.13.149) <small>(from data)</small>, at 649,875 to 650,675
_RS23735
Position (kb) 648
649
650 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 647.563 kb on + strand, within RR42_RS23720 at 647.564 kb on - strand, within RR42_RS23720 at 647.605 kb on - strand, within RR42_RS23720 at 647.616 kb on - strand, within RR42_RS23720 at 647.739 kb on + strand, within RR42_RS23720 at 647.739 kb on + strand, within RR42_RS23720 at 647.739 kb on + strand, within RR42_RS23720 at 647.739 kb on + strand, within RR42_RS23720 at 647.740 kb on - strand, within RR42_RS23720 at 647.740 kb on - strand, within RR42_RS23720 at 647.740 kb on - strand, within RR42_RS23720 at 647.783 kb on + strand, within RR42_RS23720 at 647.784 kb on - strand, within RR42_RS23720 at 647.784 kb on - strand, within RR42_RS23720 at 647.784 kb on - strand, within RR42_RS23720 at 647.784 kb on - strand, within RR42_RS23720 at 647.814 kb on + strand, within RR42_RS23720 at 647.834 kb on + strand, within RR42_RS23720 at 647.834 kb on + strand, within RR42_RS23720 at 647.835 kb on - strand, within RR42_RS23720 at 647.835 kb on - strand, within RR42_RS23720 at 647.835 kb on - strand, within RR42_RS23720 at 647.853 kb on + strand, within RR42_RS23720 at 647.865 kb on + strand, within RR42_RS23720 at 647.865 kb on + strand, within RR42_RS23720 at 648.008 kb on - strand, within RR42_RS23720 at 648.243 kb on - strand at 648.243 kb on - strand at 648.316 kb on + strand at 648.316 kb on + strand at 648.316 kb on + strand at 648.388 kb on + strand at 648.389 kb on - strand at 648.402 kb on + strand at 648.402 kb on + strand at 648.402 kb on + strand at 648.402 kb on + strand at 648.403 kb on - strand at 648.476 kb on + strand at 648.479 kb on - strand at 648.560 kb on + strand at 648.560 kb on + strand at 648.560 kb on + strand at 648.560 kb on + strand at 648.560 kb on + strand at 648.560 kb on + strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.701 kb on + strand, within RR42_RS23725 at 648.702 kb on - strand, within RR42_RS23725 at 648.702 kb on - strand, within RR42_RS23725 at 648.854 kb on + strand, within RR42_RS23725 at 648.855 kb on - strand, within RR42_RS23725 at 648.855 kb on - strand, within RR42_RS23725 at 648.861 kb on - strand, within RR42_RS23725 at 648.932 kb on + strand, within RR42_RS23725 at 648.932 kb on + strand, within RR42_RS23725 at 648.933 kb on - strand, within RR42_RS23725 at 649.043 kb on + strand, within RR42_RS23725 at 649.043 kb on + strand, within RR42_RS23725 at 649.388 kb on + strand, within RR42_RS23725 at 649.388 kb on + strand, within RR42_RS23725 at 649.614 kb on - strand, within RR42_RS23730 at 649.698 kb on + strand, within RR42_RS23730 at 649.699 kb on - strand, within RR42_RS23730 at 649.699 kb on - strand, within RR42_RS23730 at 649.857 kb on + strand at 649.857 kb on + strand at 649.857 kb on + strand at 649.858 kb on - strand at 649.858 kb on - strand at 649.928 kb on + strand at 649.987 kb on + strand, within RR42_RS23735 at 649.987 kb on + strand, within RR42_RS23735 at 650.038 kb on - strand, within RR42_RS23735 at 650.038 kb on - strand, within RR42_RS23735 at 650.097 kb on + strand, within RR42_RS23735 at 650.097 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.134 kb on - strand, within RR42_RS23735 at 650.134 kb on - strand, within RR42_RS23735 at 650.196 kb on + strand, within RR42_RS23735 at 650.197 kb on - strand, within RR42_RS23735 at 650.197 kb on - strand, within RR42_RS23735 at 650.226 kb on + strand, within RR42_RS23735 at 650.226 kb on + strand, within RR42_RS23735 at 650.410 kb on - strand, within RR42_RS23735
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 647,563 + RR42_RS23720 0.17 +1.3 647,564 - RR42_RS23720 0.17 +1.1 647,605 - RR42_RS23720 0.22 -0.1 647,616 - RR42_RS23720 0.23 -0.1 647,739 + RR42_RS23720 0.37 -0.6 647,739 + RR42_RS23720 0.37 +0.1 647,739 + RR42_RS23720 0.37 +0.5 647,739 + RR42_RS23720 0.37 -1.8 647,740 - RR42_RS23720 0.37 +0.5 647,740 - RR42_RS23720 0.37 -0.6 647,740 - RR42_RS23720 0.37 +1.6 647,783 + RR42_RS23720 0.42 +0.3 647,784 - RR42_RS23720 0.42 +0.1 647,784 - RR42_RS23720 0.42 -0.5 647,784 - RR42_RS23720 0.42 -0.2 647,784 - RR42_RS23720 0.42 +0.7 647,814 + RR42_RS23720 0.46 +0.8 647,834 + RR42_RS23720 0.48 +3.4 647,834 + RR42_RS23720 0.48 +0.8 647,835 - RR42_RS23720 0.48 +0.2 647,835 - RR42_RS23720 0.48 +0.5 647,835 - RR42_RS23720 0.48 +0.8 647,853 + RR42_RS23720 0.50 -1.2 647,865 + RR42_RS23720 0.52 +2.0 647,865 + RR42_RS23720 0.52 +1.0 648,008 - RR42_RS23720 0.68 -0.6 648,243 - -0.1 648,243 - -0.4 648,316 + +2.1 648,316 + +0.1 648,316 + +0.8 648,388 + +0.6 648,389 - -0.1 648,402 + -0.1 648,402 + -1.9 648,402 + +0.3 648,402 + +1.4 648,403 - -0.2 648,476 + +0.0 648,479 - -0.0 648,560 + -0.4 648,560 + +0.2 648,560 + +1.0 648,560 + +0.9 648,560 + +0.1 648,560 + -0.6 648,561 - +1.2 648,561 - +1.6 648,561 - +0.4 648,561 - +0.1 648,561 - -0.4 648,561 - +1.0 648,561 - +0.9 648,701 + RR42_RS23725 0.14 -0.3 648,702 - RR42_RS23725 0.14 -0.2 648,702 - RR42_RS23725 0.14 +0.3 648,854 + RR42_RS23725 0.30 -0.2 648,855 - RR42_RS23725 0.30 -0.8 648,855 - RR42_RS23725 0.30 +0.4 648,861 - RR42_RS23725 0.30 -0.7 648,932 + RR42_RS23725 0.37 -1.9 648,932 + RR42_RS23725 0.37 +0.8 648,933 - RR42_RS23725 0.37 +0.6 649,043 + RR42_RS23725 0.48 -0.7 649,043 + RR42_RS23725 0.48 +0.1 649,388 + RR42_RS23725 0.83 +0.2 649,388 + RR42_RS23725 0.83 +0.2 649,614 - RR42_RS23730 0.14 +0.5 649,698 + RR42_RS23730 0.43 +0.8 649,699 - RR42_RS23730 0.44 +2.1 649,699 - RR42_RS23730 0.44 -1.6 649,857 + +0.1 649,857 + +0.9 649,857 + -0.3 649,858 - +0.3 649,858 - -0.5 649,928 + +0.5 649,987 + RR42_RS23735 0.14 +0.0 649,987 + RR42_RS23735 0.14 +0.3 650,038 - RR42_RS23735 0.20 -2.1 650,038 - RR42_RS23735 0.20 -1.6 650,097 + RR42_RS23735 0.28 -1.0 650,097 + RR42_RS23735 0.28 +0.4 650,133 + RR42_RS23735 0.32 +1.9 650,133 + RR42_RS23735 0.32 -0.6 650,133 + RR42_RS23735 0.32 -0.1 650,133 + RR42_RS23735 0.32 -0.3 650,134 - RR42_RS23735 0.32 -2.1 650,134 - RR42_RS23735 0.32 +0.5 650,196 + RR42_RS23735 0.40 +0.4 650,197 - RR42_RS23735 0.40 -0.8 650,197 - RR42_RS23735 0.40 +0.2 650,226 + RR42_RS23735 0.44 -0.2 650,226 + RR42_RS23735 0.44 -1.4 650,410 - RR42_RS23735 0.67 +0.0
Or see this region's nucleotide sequence