Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23710 and RR42_RS23715 are separated by 216 nucleotides RR42_RS23715 and RR42_RS23720 are separated by 206 nucleotides RR42_RS23720 and RR42_RS23725 are separated by 270 nucleotides
RR42_RS23710: RR42_RS23710 - enoyl-CoA hydratase, at 645,918 to 646,667
_RS23710
RR42_RS23715: RR42_RS23715 - amino acid ABC transporter permease, at 646,884 to 647,207
_RS23715
RR42_RS23720: RR42_RS23720 - hypothetical protein, at 647,414 to 648,286
_RS23720
RR42_RS23725: RR42_RS23725 - phenylacetate-CoA oxygenase, at 648,557 to 649,561
_RS23725
Position (kb) 647
648
649 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 646.420 kb on + strand, within RR42_RS23710 at 646.420 kb on + strand, within RR42_RS23710 at 646.666 kb on - strand at 646.789 kb on + strand at 646.789 kb on + strand at 646.790 kb on - strand at 646.792 kb on + strand at 646.793 kb on - strand at 646.803 kb on + strand at 646.902 kb on - strand at 646.974 kb on + strand, within RR42_RS23715 at 646.975 kb on - strand, within RR42_RS23715 at 647.010 kb on + strand, within RR42_RS23715 at 647.010 kb on + strand, within RR42_RS23715 at 647.011 kb on - strand, within RR42_RS23715 at 647.011 kb on - strand, within RR42_RS23715 at 647.011 kb on - strand, within RR42_RS23715 at 647.047 kb on - strand, within RR42_RS23715 at 647.048 kb on + strand, within RR42_RS23715 at 647.048 kb on + strand, within RR42_RS23715 at 647.159 kb on + strand, within RR42_RS23715 at 647.160 kb on - strand, within RR42_RS23715 at 647.160 kb on - strand, within RR42_RS23715 at 647.160 kb on - strand, within RR42_RS23715 at 647.517 kb on + strand, within RR42_RS23720 at 647.518 kb on - strand, within RR42_RS23720 at 647.563 kb on + strand, within RR42_RS23720 at 647.564 kb on - strand, within RR42_RS23720 at 647.605 kb on - strand, within RR42_RS23720 at 647.616 kb on - strand, within RR42_RS23720 at 647.739 kb on + strand, within RR42_RS23720 at 647.739 kb on + strand, within RR42_RS23720 at 647.739 kb on + strand, within RR42_RS23720 at 647.739 kb on + strand, within RR42_RS23720 at 647.740 kb on - strand, within RR42_RS23720 at 647.740 kb on - strand, within RR42_RS23720 at 647.740 kb on - strand, within RR42_RS23720 at 647.783 kb on + strand, within RR42_RS23720 at 647.784 kb on - strand, within RR42_RS23720 at 647.784 kb on - strand, within RR42_RS23720 at 647.784 kb on - strand, within RR42_RS23720 at 647.784 kb on - strand, within RR42_RS23720 at 647.814 kb on + strand, within RR42_RS23720 at 647.834 kb on + strand, within RR42_RS23720 at 647.834 kb on + strand, within RR42_RS23720 at 647.835 kb on - strand, within RR42_RS23720 at 647.835 kb on - strand, within RR42_RS23720 at 647.835 kb on - strand, within RR42_RS23720 at 647.853 kb on + strand, within RR42_RS23720 at 647.865 kb on + strand, within RR42_RS23720 at 647.865 kb on + strand, within RR42_RS23720 at 648.008 kb on - strand, within RR42_RS23720 at 648.243 kb on - strand at 648.243 kb on - strand at 648.316 kb on + strand at 648.316 kb on + strand at 648.316 kb on + strand at 648.388 kb on + strand at 648.389 kb on - strand at 648.402 kb on + strand at 648.402 kb on + strand at 648.402 kb on + strand at 648.402 kb on + strand at 648.403 kb on - strand at 648.476 kb on + strand at 648.479 kb on - strand at 648.560 kb on + strand at 648.560 kb on + strand at 648.560 kb on + strand at 648.560 kb on + strand at 648.560 kb on + strand at 648.560 kb on + strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.561 kb on - strand at 648.701 kb on + strand, within RR42_RS23725 at 648.702 kb on - strand, within RR42_RS23725 at 648.702 kb on - strand, within RR42_RS23725 at 648.854 kb on + strand, within RR42_RS23725 at 648.855 kb on - strand, within RR42_RS23725 at 648.855 kb on - strand, within RR42_RS23725 at 648.861 kb on - strand, within RR42_RS23725 at 648.932 kb on + strand, within RR42_RS23725 at 648.932 kb on + strand, within RR42_RS23725 at 648.933 kb on - strand, within RR42_RS23725 at 649.043 kb on + strand, within RR42_RS23725 at 649.043 kb on + strand, within RR42_RS23725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 646,420 + RR42_RS23710 0.67 +0.5 646,420 + RR42_RS23710 0.67 -0.4 646,666 - +1.4 646,789 + +0.8 646,789 + +0.8 646,790 - -0.3 646,792 + +0.1 646,793 - -0.9 646,803 + -0.1 646,902 - +0.1 646,974 + RR42_RS23715 0.28 -0.5 646,975 - RR42_RS23715 0.28 +0.2 647,010 + RR42_RS23715 0.39 -1.7 647,010 + RR42_RS23715 0.39 -0.2 647,011 - RR42_RS23715 0.39 +2.1 647,011 - RR42_RS23715 0.39 -0.6 647,011 - RR42_RS23715 0.39 -0.4 647,047 - RR42_RS23715 0.50 -0.6 647,048 + RR42_RS23715 0.51 -0.9 647,048 + RR42_RS23715 0.51 +1.0 647,159 + RR42_RS23715 0.85 +0.4 647,160 - RR42_RS23715 0.85 +1.4 647,160 - RR42_RS23715 0.85 +1.4 647,160 - RR42_RS23715 0.85 -0.7 647,517 + RR42_RS23720 0.12 -0.6 647,518 - RR42_RS23720 0.12 +0.5 647,563 + RR42_RS23720 0.17 +1.3 647,564 - RR42_RS23720 0.17 +1.1 647,605 - RR42_RS23720 0.22 -0.1 647,616 - RR42_RS23720 0.23 -0.1 647,739 + RR42_RS23720 0.37 -0.6 647,739 + RR42_RS23720 0.37 +0.1 647,739 + RR42_RS23720 0.37 +0.5 647,739 + RR42_RS23720 0.37 -1.8 647,740 - RR42_RS23720 0.37 +0.5 647,740 - RR42_RS23720 0.37 -0.6 647,740 - RR42_RS23720 0.37 +1.6 647,783 + RR42_RS23720 0.42 +0.3 647,784 - RR42_RS23720 0.42 +0.1 647,784 - RR42_RS23720 0.42 -0.5 647,784 - RR42_RS23720 0.42 -0.2 647,784 - RR42_RS23720 0.42 +0.7 647,814 + RR42_RS23720 0.46 +0.8 647,834 + RR42_RS23720 0.48 +3.4 647,834 + RR42_RS23720 0.48 +0.8 647,835 - RR42_RS23720 0.48 +0.2 647,835 - RR42_RS23720 0.48 +0.5 647,835 - RR42_RS23720 0.48 +0.8 647,853 + RR42_RS23720 0.50 -1.2 647,865 + RR42_RS23720 0.52 +2.0 647,865 + RR42_RS23720 0.52 +1.0 648,008 - RR42_RS23720 0.68 -0.6 648,243 - -0.1 648,243 - -0.4 648,316 + +2.1 648,316 + +0.1 648,316 + +0.8 648,388 + +0.6 648,389 - -0.1 648,402 + -0.1 648,402 + -1.9 648,402 + +0.3 648,402 + +1.4 648,403 - -0.2 648,476 + +0.0 648,479 - -0.0 648,560 + -0.4 648,560 + +0.2 648,560 + +1.0 648,560 + +0.9 648,560 + +0.1 648,560 + -0.6 648,561 - +1.2 648,561 - +1.6 648,561 - +0.4 648,561 - +0.1 648,561 - -0.4 648,561 - +1.0 648,561 - +0.9 648,701 + RR42_RS23725 0.14 -0.3 648,702 - RR42_RS23725 0.14 -0.2 648,702 - RR42_RS23725 0.14 +0.3 648,854 + RR42_RS23725 0.30 -0.2 648,855 - RR42_RS23725 0.30 -0.8 648,855 - RR42_RS23725 0.30 +0.4 648,861 - RR42_RS23725 0.30 -0.7 648,932 + RR42_RS23725 0.37 -1.9 648,932 + RR42_RS23725 0.37 +0.8 648,933 - RR42_RS23725 0.37 +0.6 649,043 + RR42_RS23725 0.48 -0.7 649,043 + RR42_RS23725 0.48 +0.1
Or see this region's nucleotide sequence