Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23300 and RR42_RS23305 are separated by 93 nucleotides RR42_RS23305 and RR42_RS23310 overlap by 4 nucleotides RR42_RS23310 and RR42_RS23315 overlap by 4 nucleotides
RR42_RS23300: RR42_RS23300 - hypothetical protein, at 557,895 to 558,860
_RS23300
RR42_RS23305: RR42_RS23305 - hypothetical protein, at 558,954 to 559,529
_RS23305
RR42_RS23310: RR42_RS23310 - cytochrome B6, at 559,526 to 560,863
_RS23310
RR42_RS23315: RR42_RS23315 - serine/threonine protein phosphatase, at 560,860 to 562,416
_RS23315
Position (kb) 559
560
561 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 558.572 kb on - strand, within RR42_RS23300 at 558.623 kb on + strand, within RR42_RS23300 at 558.788 kb on + strand at 558.789 kb on - strand at 558.793 kb on - strand at 558.920 kb on - strand at 558.920 kb on - strand at 559.317 kb on + strand, within RR42_RS23305 at 559.365 kb on + strand, within RR42_RS23305 at 559.566 kb on + strand at 559.567 kb on - strand at 559.567 kb on - strand at 559.761 kb on + strand, within RR42_RS23310 at 559.762 kb on - strand, within RR42_RS23310 at 559.762 kb on - strand, within RR42_RS23310 at 559.762 kb on - strand, within RR42_RS23310 at 560.127 kb on + strand, within RR42_RS23310 at 560.176 kb on - strand, within RR42_RS23310 at 560.406 kb on + strand, within RR42_RS23310 at 560.428 kb on - strand, within RR42_RS23310 at 560.428 kb on - strand, within RR42_RS23310 at 560.448 kb on + strand, within RR42_RS23310 at 560.449 kb on - strand, within RR42_RS23310 at 560.449 kb on - strand, within RR42_RS23310 at 560.457 kb on + strand, within RR42_RS23310 at 560.457 kb on + strand, within RR42_RS23310 at 560.457 kb on + strand, within RR42_RS23310 at 560.458 kb on - strand, within RR42_RS23310 at 560.899 kb on - strand at 560.944 kb on - strand at 560.945 kb on + strand at 560.945 kb on + strand at 560.945 kb on + strand at 561.085 kb on + strand, within RR42_RS23315 at 561.207 kb on - strand, within RR42_RS23315 at 561.356 kb on - strand, within RR42_RS23315 at 561.488 kb on + strand, within RR42_RS23315 at 561.489 kb on - strand, within RR42_RS23315 at 561.843 kb on - strand, within RR42_RS23315
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 558,572 - RR42_RS23300 0.70 -0.3 558,623 + RR42_RS23300 0.75 -3.8 558,788 + +0.7 558,789 - -0.9 558,793 - +0.3 558,920 - -1.0 558,920 - +0.9 559,317 + RR42_RS23305 0.63 +0.8 559,365 + RR42_RS23305 0.71 -2.5 559,566 + -0.6 559,567 - +0.4 559,567 - -1.1 559,761 + RR42_RS23310 0.18 +0.1 559,762 - RR42_RS23310 0.18 +1.1 559,762 - RR42_RS23310 0.18 -0.3 559,762 - RR42_RS23310 0.18 -0.5 560,127 + RR42_RS23310 0.45 -0.2 560,176 - RR42_RS23310 0.49 +0.1 560,406 + RR42_RS23310 0.66 -0.4 560,428 - RR42_RS23310 0.67 -0.7 560,428 - RR42_RS23310 0.67 -0.6 560,448 + RR42_RS23310 0.69 +0.1 560,449 - RR42_RS23310 0.69 -0.1 560,449 - RR42_RS23310 0.69 +0.2 560,457 + RR42_RS23310 0.70 -0.8 560,457 + RR42_RS23310 0.70 -1.3 560,457 + RR42_RS23310 0.70 +0.9 560,458 - RR42_RS23310 0.70 -0.9 560,899 - +0.4 560,944 - -0.5 560,945 + -0.5 560,945 + -0.2 560,945 + -1.1 561,085 + RR42_RS23315 0.14 -0.2 561,207 - RR42_RS23315 0.22 +0.9 561,356 - RR42_RS23315 0.32 -2.2 561,488 + RR42_RS23315 0.40 -0.1 561,489 - RR42_RS23315 0.40 +0.2 561,843 - RR42_RS23315 0.63 -1.0
Or see this region's nucleotide sequence