Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22905 and RR42_RS22910 are separated by 14 nucleotides RR42_RS22910 and RR42_RS22915 are separated by 4 nucleotides RR42_RS22915 and RR42_RS22920 are separated by 69 nucleotides RR42_RS22920 and RR42_RS22925 are separated by 95 nucleotides
RR42_RS22905: RR42_RS22905 - myristoyl transferase, at 471,896 to 472,885
_RS22905
RR42_RS22910: RR42_RS22910 - nitrate ABC transporter ATPase, at 472,900 to 473,673
_RS22910
RR42_RS22915: RR42_RS22915 - ABC transporter permease, at 473,678 to 474,517
_RS22915
RR42_RS22920: RR42_RS22920 - hypothetical protein, at 474,587 to 475,024
_RS22920
RR42_RS22925: RR42_RS22925 - RND transporter, at 475,120 to 476,193
_RS22925
Position (kb) 473
474
475 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 472.724 kb on + strand, within RR42_RS22905 at 472.725 kb on - strand, within RR42_RS22905 at 472.856 kb on + strand at 472.856 kb on + strand at 472.857 kb on - strand at 472.857 kb on - strand at 472.859 kb on + strand at 472.859 kb on + strand at 472.884 kb on - strand at 472.897 kb on + strand at 472.897 kb on + strand at 472.898 kb on - strand at 472.898 kb on - strand at 472.898 kb on - strand at 472.898 kb on - strand at 472.940 kb on - strand at 473.195 kb on + strand, within RR42_RS22910 at 473.196 kb on - strand, within RR42_RS22910 at 473.354 kb on + strand, within RR42_RS22910 at 473.355 kb on - strand, within RR42_RS22910 at 473.500 kb on + strand, within RR42_RS22910 at 473.500 kb on + strand, within RR42_RS22910 at 473.501 kb on - strand, within RR42_RS22910 at 473.501 kb on - strand, within RR42_RS22910 at 473.621 kb on - strand at 473.621 kb on - strand at 473.662 kb on + strand at 473.663 kb on - strand at 473.688 kb on + strand at 473.689 kb on - strand at 474.174 kb on + strand, within RR42_RS22915 at 474.174 kb on + strand, within RR42_RS22915 at 474.174 kb on + strand, within RR42_RS22915 at 474.175 kb on - strand, within RR42_RS22915 at 474.659 kb on - strand, within RR42_RS22920 at 474.744 kb on - strand, within RR42_RS22920 at 475.199 kb on + strand at 475.200 kb on - strand at 475.302 kb on - strand, within RR42_RS22925 at 475.302 kb on - strand, within RR42_RS22925 at 475.318 kb on + strand, within RR42_RS22925 at 475.336 kb on + strand, within RR42_RS22925 at 475.337 kb on - strand, within RR42_RS22925 at 475.337 kb on - strand, within RR42_RS22925
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 472,724 + RR42_RS22905 0.84 -0.6 472,725 - RR42_RS22905 0.84 -1.3 472,856 + +0.4 472,856 + +0.7 472,857 - +0.1 472,857 - +0.3 472,859 + -1.1 472,859 + +0.7 472,884 - -0.6 472,897 + +0.6 472,897 + +3.0 472,898 - +0.1 472,898 - +0.1 472,898 - -0.0 472,898 - -1.1 472,940 - -0.5 473,195 + RR42_RS22910 0.38 -0.6 473,196 - RR42_RS22910 0.38 -0.2 473,354 + RR42_RS22910 0.59 +1.3 473,355 - RR42_RS22910 0.59 -0.8 473,500 + RR42_RS22910 0.78 +0.1 473,500 + RR42_RS22910 0.78 -0.7 473,501 - RR42_RS22910 0.78 +0.7 473,501 - RR42_RS22910 0.78 -1.2 473,621 - +0.1 473,621 - -1.8 473,662 + +0.3 473,663 - -0.2 473,688 + -0.6 473,689 - -1.2 474,174 + RR42_RS22915 0.59 -0.1 474,174 + RR42_RS22915 0.59 +0.5 474,174 + RR42_RS22915 0.59 +0.0 474,175 - RR42_RS22915 0.59 +2.9 474,659 - RR42_RS22920 0.16 -0.2 474,744 - RR42_RS22920 0.36 +0.2 475,199 + +0.4 475,200 - +0.3 475,302 - RR42_RS22925 0.17 +0.2 475,302 - RR42_RS22925 0.17 -1.4 475,318 + RR42_RS22925 0.18 +0.6 475,336 + RR42_RS22925 0.20 +1.4 475,337 - RR42_RS22925 0.20 -0.3 475,337 - RR42_RS22925 0.20 -1.1
Or see this region's nucleotide sequence