Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22850 and RR42_RS22855 are separated by 416 nucleotides RR42_RS22855 and RR42_RS22860 are separated by 81 nucleotides RR42_RS22860 and RR42_RS22865 are separated by 57 nucleotides
RR42_RS22850: RR42_RS22850 - amino acid dehydrogenase, at 457,915 to 458,958
_RS22850
RR42_RS22855: RR42_RS22855 - sulfate transporter, at 459,375 to 459,812
_RS22855
RR42_RS22860: RR42_RS22860 - l-threonine 3-dehydrogenase, at 459,894 to 460,949
_RS22860
RR42_RS22865: RR42_RS22865 - 2-amino-3-ketobutyrate CoA ligase, at 461,007 to 462,209
_RS22865
Position (kb) 459
460
461 Strain fitness (log2 ratio) -2
-1
0
1 at 459.030 kb on + strand at 459.112 kb on - strand at 459.195 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.235 kb on - strand at 459.235 kb on - strand at 459.235 kb on - strand at 459.282 kb on + strand at 459.284 kb on + strand at 459.284 kb on + strand at 459.284 kb on + strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.286 kb on + strand at 459.287 kb on - strand at 459.287 kb on - strand at 459.300 kb on + strand at 459.507 kb on + strand, within RR42_RS22855 at 459.507 kb on + strand, within RR42_RS22855 at 459.508 kb on - strand, within RR42_RS22855 at 459.508 kb on - strand, within RR42_RS22855 at 459.513 kb on + strand, within RR42_RS22855 at 459.513 kb on + strand, within RR42_RS22855 at 459.514 kb on - strand, within RR42_RS22855 at 459.646 kb on + strand, within RR42_RS22855 at 459.647 kb on - strand, within RR42_RS22855 at 459.647 kb on - strand, within RR42_RS22855 at 459.661 kb on - strand, within RR42_RS22855 at 459.782 kb on + strand at 459.782 kb on + strand at 459.782 kb on + strand at 459.783 kb on - strand at 459.783 kb on - strand at 459.805 kb on - strand at 459.805 kb on - strand at 459.908 kb on + strand at 460.064 kb on - strand, within RR42_RS22860 at 460.090 kb on + strand, within RR42_RS22860 at 460.090 kb on + strand, within RR42_RS22860 at 460.091 kb on - strand, within RR42_RS22860 at 460.579 kb on + strand, within RR42_RS22860 at 460.676 kb on + strand, within RR42_RS22860 at 460.676 kb on + strand, within RR42_RS22860 at 460.676 kb on + strand, within RR42_RS22860 at 460.752 kb on + strand, within RR42_RS22860 at 460.752 kb on + strand, within RR42_RS22860 at 460.752 kb on + strand, within RR42_RS22860 at 460.753 kb on - strand, within RR42_RS22860 at 460.756 kb on + strand, within RR42_RS22860 at 460.757 kb on - strand, within RR42_RS22860 at 460.820 kb on + strand, within RR42_RS22860 at 460.820 kb on + strand, within RR42_RS22860 at 461.079 kb on - strand at 461.153 kb on - strand, within RR42_RS22865 at 461.153 kb on - strand, within RR42_RS22865 at 461.249 kb on + strand, within RR42_RS22865 at 461.250 kb on - strand, within RR42_RS22865 at 461.452 kb on + strand, within RR42_RS22865 at 461.453 kb on - strand, within RR42_RS22865
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 459,030 + -0.3 459,112 - +0.0 459,195 + -0.7 459,234 + -0.1 459,234 + +0.9 459,234 + -0.1 459,234 + +0.2 459,234 + +0.6 459,234 + -0.2 459,234 + -1.0 459,235 - -0.6 459,235 - -2.0 459,235 - -0.4 459,282 + -0.3 459,284 + +1.3 459,284 + +0.9 459,284 + +0.2 459,285 - +0.2 459,285 - +0.5 459,285 - +0.3 459,285 - +0.3 459,285 - -1.1 459,285 - +0.6 459,286 + +0.7 459,287 - +0.1 459,287 - -0.3 459,300 + -1.1 459,507 + RR42_RS22855 0.30 -0.0 459,507 + RR42_RS22855 0.30 -0.4 459,508 - RR42_RS22855 0.30 +0.9 459,508 - RR42_RS22855 0.30 +0.1 459,513 + RR42_RS22855 0.32 -0.7 459,513 + RR42_RS22855 0.32 -0.1 459,514 - RR42_RS22855 0.32 +0.4 459,646 + RR42_RS22855 0.62 -0.7 459,647 - RR42_RS22855 0.62 -0.0 459,647 - RR42_RS22855 0.62 -0.4 459,661 - RR42_RS22855 0.65 +0.0 459,782 + +0.2 459,782 + -0.2 459,782 + -0.2 459,783 - +0.3 459,783 - +0.1 459,805 - -0.4 459,805 - +0.0 459,908 + +0.3 460,064 - RR42_RS22860 0.16 +0.7 460,090 + RR42_RS22860 0.19 +0.1 460,090 + RR42_RS22860 0.19 +0.2 460,091 - RR42_RS22860 0.19 -1.4 460,579 + RR42_RS22860 0.65 -1.4 460,676 + RR42_RS22860 0.74 -0.5 460,676 + RR42_RS22860 0.74 -1.9 460,676 + RR42_RS22860 0.74 -1.4 460,752 + RR42_RS22860 0.81 -1.1 460,752 + RR42_RS22860 0.81 -0.4 460,752 + RR42_RS22860 0.81 -0.2 460,753 - RR42_RS22860 0.81 +0.4 460,756 + RR42_RS22860 0.82 -0.8 460,757 - RR42_RS22860 0.82 +0.8 460,820 + RR42_RS22860 0.88 -1.8 460,820 + RR42_RS22860 0.88 +1.4 461,079 - -0.9 461,153 - RR42_RS22865 0.12 -1.7 461,153 - RR42_RS22865 0.12 +0.1 461,249 + RR42_RS22865 0.20 +1.3 461,250 - RR42_RS22865 0.20 +0.4 461,452 + RR42_RS22865 0.37 +0.5 461,453 - RR42_RS22865 0.37 -1.2
Or see this region's nucleotide sequence