Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22720 and RR42_RS22725 are separated by 800 nucleotides RR42_RS22725 and RR42_RS22730 are separated by 480 nucleotides
RR42_RS22720: RR42_RS22720 - hypothetical protein, at 431,600 to 431,977
_RS22720
RR42_RS22725: RR42_RS22725 - phosphatidylethanolamine-binding protein, at 432,778 to 433,332
_RS22725
RR42_RS22730: RR42_RS22730 - pyridine nucleotide-disulfide oxidoreductase, at 433,813 to 435,018
_RS22730
Position (kb) 432
433
434 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 432.013 kb on + strand at 432.031 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.046 kb on + strand at 432.095 kb on - strand at 432.095 kb on - strand at 432.125 kb on - strand at 432.125 kb on - strand at 432.148 kb on + strand at 432.148 kb on + strand at 432.149 kb on - strand at 432.193 kb on + strand at 432.194 kb on - strand at 432.300 kb on - strand at 432.494 kb on + strand at 432.495 kb on - strand at 432.495 kb on - strand at 432.536 kb on + strand at 432.536 kb on + strand at 432.613 kb on + strand at 432.614 kb on - strand at 432.626 kb on - strand at 432.631 kb on + strand at 432.650 kb on + strand at 432.651 kb on - strand at 432.651 kb on - strand at 432.684 kb on - strand at 432.715 kb on + strand at 432.715 kb on + strand at 432.715 kb on + strand at 432.744 kb on + strand at 432.847 kb on + strand, within RR42_RS22725 at 432.847 kb on + strand, within RR42_RS22725 at 432.847 kb on + strand, within RR42_RS22725 at 432.847 kb on + strand, within RR42_RS22725 at 432.910 kb on + strand, within RR42_RS22725 at 432.910 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.922 kb on - strand, within RR42_RS22725 at 432.922 kb on - strand, within RR42_RS22725 at 432.922 kb on - strand, within RR42_RS22725 at 433.034 kb on + strand, within RR42_RS22725 at 433.039 kb on + strand, within RR42_RS22725 at 433.057 kb on + strand, within RR42_RS22725 at 433.059 kb on + strand, within RR42_RS22725 at 433.060 kb on - strand, within RR42_RS22725 at 433.139 kb on + strand, within RR42_RS22725 at 433.198 kb on + strand, within RR42_RS22725 at 433.198 kb on + strand, within RR42_RS22725 at 433.199 kb on - strand, within RR42_RS22725 at 433.199 kb on - strand, within RR42_RS22725 at 433.322 kb on - strand at 433.342 kb on + strand at 433.343 kb on - strand at 433.433 kb on + strand at 433.434 kb on - strand at 433.793 kb on - strand at 433.819 kb on - strand at 433.825 kb on - strand at 433.963 kb on + strand, within RR42_RS22730 at 434.116 kb on + strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.136 kb on + strand, within RR42_RS22730 at 434.233 kb on - strand, within RR42_RS22730 at 434.233 kb on - strand, within RR42_RS22730 at 434.274 kb on + strand, within RR42_RS22730 at 434.275 kb on - strand, within RR42_RS22730
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 432,013 + +1.1 432,031 + +0.1 432,034 + +0.2 432,034 + +0.9 432,034 + -0.4 432,034 + +0.4 432,034 + -1.6 432,034 + +1.4 432,034 + +0.5 432,034 + -0.6 432,035 - -1.2 432,035 - +0.1 432,035 - -0.3 432,035 - +0.5 432,035 - -0.4 432,046 + -0.2 432,095 - -0.3 432,095 - +0.4 432,125 - -0.6 432,125 - +0.3 432,148 + +1.1 432,148 + -0.4 432,149 - -1.0 432,193 + +0.5 432,194 - +0.0 432,300 - -1.4 432,494 + +0.0 432,495 - -0.2 432,495 - -0.4 432,536 + -0.3 432,536 + +0.7 432,613 + -0.0 432,614 - -1.2 432,626 - -0.1 432,631 + -0.6 432,650 + +0.7 432,651 - +0.9 432,651 - +0.6 432,684 - +0.1 432,715 + -0.0 432,715 + +0.4 432,715 + -1.0 432,744 + +1.8 432,847 + RR42_RS22725 0.12 -0.6 432,847 + RR42_RS22725 0.12 -0.6 432,847 + RR42_RS22725 0.12 -0.8 432,847 + RR42_RS22725 0.12 -0.5 432,910 + RR42_RS22725 0.24 -0.6 432,910 + RR42_RS22725 0.24 -0.6 432,921 + RR42_RS22725 0.26 +0.5 432,921 + RR42_RS22725 0.26 -0.8 432,921 + RR42_RS22725 0.26 -0.7 432,921 + RR42_RS22725 0.26 -0.1 432,922 - RR42_RS22725 0.26 -1.2 432,922 - RR42_RS22725 0.26 +0.7 432,922 - RR42_RS22725 0.26 +0.1 433,034 + RR42_RS22725 0.46 +0.4 433,039 + RR42_RS22725 0.47 +0.2 433,057 + RR42_RS22725 0.50 -1.2 433,059 + RR42_RS22725 0.51 -0.9 433,060 - RR42_RS22725 0.51 -2.9 433,139 + RR42_RS22725 0.65 +0.8 433,198 + RR42_RS22725 0.76 +0.0 433,198 + RR42_RS22725 0.76 +0.1 433,199 - RR42_RS22725 0.76 -0.3 433,199 - RR42_RS22725 0.76 +0.4 433,322 - -0.8 433,342 + -1.1 433,343 - -0.1 433,433 + -0.2 433,434 - -0.4 433,793 - -2.2 433,819 - +0.5 433,825 - +0.4 433,963 + RR42_RS22730 0.12 +1.4 434,116 + RR42_RS22730 0.25 -0.4 434,117 - RR42_RS22730 0.25 +0.1 434,117 - RR42_RS22730 0.25 +0.5 434,117 - RR42_RS22730 0.25 +0.5 434,117 - RR42_RS22730 0.25 -0.9 434,136 + RR42_RS22730 0.27 -1.2 434,233 - RR42_RS22730 0.35 +0.5 434,233 - RR42_RS22730 0.35 +0.2 434,274 + RR42_RS22730 0.38 +0.8 434,275 - RR42_RS22730 0.38 +0.4
Or see this region's nucleotide sequence