Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22415 and RR42_RS22420 are separated by 141 nucleotides RR42_RS22420 and RR42_RS22425 are separated by 312 nucleotides RR42_RS22425 and RR42_RS22430 are separated by 8 nucleotides
RR42_RS22415: RR42_RS22415 - hypothetical protein, at 364,047 to 364,883
_RS22415
RR42_RS22420: RR42_RS22420 - penicillin-binding protein, at 365,025 to 367,448
_RS22420
RR42_RS22425: RR42_RS22425 - CDP-alcohol phosphatidyltransferase, at 367,761 to 368,429
_RS22425
RR42_RS22430: RR42_RS22430 - 2OG-Fe(II) oxygenase, at 368,438 to 369,280
_RS22430
Position (kb)
365
366
367
368 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 364.269 kb on + strand, within RR42_RS22415 at 364.270 kb on - strand, within RR42_RS22415 at 364.587 kb on + strand, within RR42_RS22415 at 364.588 kb on - strand, within RR42_RS22415 at 364.593 kb on + strand, within RR42_RS22415 at 364.593 kb on + strand, within RR42_RS22415 at 364.593 kb on + strand, within RR42_RS22415 at 364.620 kb on - strand, within RR42_RS22415 at 364.707 kb on + strand, within RR42_RS22415 at 364.708 kb on - strand, within RR42_RS22415 at 364.708 kb on - strand, within RR42_RS22415 at 364.737 kb on + strand, within RR42_RS22415 at 364.737 kb on + strand, within RR42_RS22415 at 364.762 kb on - strand, within RR42_RS22415 at 364.779 kb on + strand, within RR42_RS22415 at 364.821 kb on + strand at 364.822 kb on - strand at 364.833 kb on + strand at 364.834 kb on - strand at 365.139 kb on + strand at 365.139 kb on + strand at 365.139 kb on + strand at 365.139 kb on + strand at 365.286 kb on - strand, within RR42_RS22420 at 365.388 kb on - strand, within RR42_RS22420 at 365.529 kb on + strand, within RR42_RS22420 at 365.529 kb on + strand, within RR42_RS22420 at 365.556 kb on + strand, within RR42_RS22420 at 365.557 kb on - strand, within RR42_RS22420 at 365.557 kb on - strand, within RR42_RS22420 at 365.557 kb on - strand, within RR42_RS22420 at 365.580 kb on + strand, within RR42_RS22420 at 365.581 kb on - strand, within RR42_RS22420 at 365.616 kb on + strand, within RR42_RS22420 at 365.616 kb on + strand, within RR42_RS22420 at 365.616 kb on + strand, within RR42_RS22420 at 365.691 kb on + strand, within RR42_RS22420 at 365.692 kb on - strand, within RR42_RS22420 at 365.692 kb on - strand, within RR42_RS22420 at 365.694 kb on + strand, within RR42_RS22420 at 365.694 kb on + strand, within RR42_RS22420 at 365.694 kb on + strand, within RR42_RS22420 at 365.694 kb on + strand, within RR42_RS22420 at 365.695 kb on - strand, within RR42_RS22420 at 365.695 kb on - strand, within RR42_RS22420 at 365.695 kb on - strand, within RR42_RS22420 at 365.763 kb on + strand, within RR42_RS22420 at 365.788 kb on - strand, within RR42_RS22420 at 365.788 kb on - strand, within RR42_RS22420 at 365.788 kb on - strand, within RR42_RS22420 at 365.914 kb on - strand, within RR42_RS22420 at 365.914 kb on - strand, within RR42_RS22420 at 365.914 kb on - strand, within RR42_RS22420 at 365.914 kb on - strand, within RR42_RS22420 at 365.923 kb on - strand, within RR42_RS22420 at 365.923 kb on - strand, within RR42_RS22420 at 366.259 kb on + strand, within RR42_RS22420 at 366.259 kb on + strand, within RR42_RS22420 at 366.495 kb on + strand, within RR42_RS22420 at 366.657 kb on + strand, within RR42_RS22420 at 366.997 kb on - strand, within RR42_RS22420 at 367.138 kb on + strand, within RR42_RS22420 at 367.618 kb on + strand at 367.618 kb on + strand at 367.619 kb on - strand at 367.619 kb on - strand at 367.640 kb on + strand at 367.712 kb on + strand at 367.713 kb on - strand at 367.715 kb on + strand at 367.715 kb on + strand at 367.716 kb on - strand at 367.716 kb on - strand at 367.809 kb on - strand at 367.809 kb on - strand at 367.953 kb on + strand, within RR42_RS22425 at 367.953 kb on + strand, within RR42_RS22425 at 368.038 kb on + strand, within RR42_RS22425 at 368.277 kb on + strand, within RR42_RS22425 at 368.277 kb on + strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.377 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 364,269 + RR42_RS22415 0.27 -0.6 364,270 - RR42_RS22415 0.27 -0.4 364,587 + RR42_RS22415 0.65 -0.3 364,588 - RR42_RS22415 0.65 -2.6 364,593 + RR42_RS22415 0.65 -0.3 364,593 + RR42_RS22415 0.65 -0.1 364,593 + RR42_RS22415 0.65 -0.3 364,620 - RR42_RS22415 0.68 -0.5 364,707 + RR42_RS22415 0.79 +0.2 364,708 - RR42_RS22415 0.79 -0.6 364,708 - RR42_RS22415 0.79 -0.2 364,737 + RR42_RS22415 0.82 -2.0 364,737 + RR42_RS22415 0.82 -0.6 364,762 - RR42_RS22415 0.85 +0.5 364,779 + RR42_RS22415 0.87 -1.6 364,821 + -0.3 364,822 - -0.4 364,833 + -0.8 364,834 - -0.8 365,139 + +0.6 365,139 + -0.9 365,139 + +1.6 365,139 + -0.6 365,286 - RR42_RS22420 0.11 -0.9 365,388 - RR42_RS22420 0.15 -2.4 365,529 + RR42_RS22420 0.21 +0.3 365,529 + RR42_RS22420 0.21 -0.8 365,556 + RR42_RS22420 0.22 -0.5 365,557 - RR42_RS22420 0.22 -0.2 365,557 - RR42_RS22420 0.22 -0.3 365,557 - RR42_RS22420 0.22 +1.6 365,580 + RR42_RS22420 0.23 -2.0 365,581 - RR42_RS22420 0.23 -0.7 365,616 + RR42_RS22420 0.24 +0.1 365,616 + RR42_RS22420 0.24 -0.3 365,616 + RR42_RS22420 0.24 -0.7 365,691 + RR42_RS22420 0.27 -1.3 365,692 - RR42_RS22420 0.28 -0.8 365,692 - RR42_RS22420 0.28 -0.5 365,694 + RR42_RS22420 0.28 -0.8 365,694 + RR42_RS22420 0.28 -1.0 365,694 + RR42_RS22420 0.28 -0.3 365,694 + RR42_RS22420 0.28 -1.0 365,695 - RR42_RS22420 0.28 +0.3 365,695 - RR42_RS22420 0.28 +1.1 365,695 - RR42_RS22420 0.28 +1.3 365,763 + RR42_RS22420 0.30 -0.1 365,788 - RR42_RS22420 0.31 -0.4 365,788 - RR42_RS22420 0.31 +1.0 365,788 - RR42_RS22420 0.31 +0.7 365,914 - RR42_RS22420 0.37 -0.0 365,914 - RR42_RS22420 0.37 -0.4 365,914 - RR42_RS22420 0.37 +1.0 365,914 - RR42_RS22420 0.37 -0.1 365,923 - RR42_RS22420 0.37 +0.0 365,923 - RR42_RS22420 0.37 -0.3 366,259 + RR42_RS22420 0.51 -0.9 366,259 + RR42_RS22420 0.51 +1.6 366,495 + RR42_RS22420 0.61 -0.8 366,657 + RR42_RS22420 0.67 -0.2 366,997 - RR42_RS22420 0.81 +0.5 367,138 + RR42_RS22420 0.87 -0.1 367,618 + +2.4 367,618 + -1.1 367,619 - -0.1 367,619 - +0.7 367,640 + -0.0 367,712 + -1.4 367,713 - -0.4 367,715 + -0.5 367,715 + -0.5 367,716 - +0.3 367,716 - -0.1 367,809 - +0.7 367,809 - -1.5 367,953 + RR42_RS22425 0.29 -0.2 367,953 + RR42_RS22425 0.29 -0.0 368,038 + RR42_RS22425 0.41 -1.2 368,277 + RR42_RS22425 0.77 -1.1 368,277 + RR42_RS22425 0.77 +0.1 368,278 - RR42_RS22425 0.77 -0.2 368,278 - RR42_RS22425 0.77 -0.5 368,278 - RR42_RS22425 0.77 -0.4 368,377 - -0.5
Or see this region's nucleotide sequence