Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22320 and RR42_RS22325 overlap by 4 nucleotides RR42_RS22325 and RR42_RS22330 are separated by 31 nucleotides RR42_RS22330 and RR42_RS22335 are separated by 414 nucleotides
RR42_RS22320: RR42_RS22320 - MFS transporter, at 347,867 to 349,171
_RS22320
RR42_RS22325: RR42_RS22325 - hypothetical protein, at 349,168 to 350,265
_RS22325
RR42_RS22330: RR42_RS22330 - ester cyclase, at 350,297 to 350,800
_RS22330
RR42_RS22335: RR42_RS22335 - hemagglutinin, at 351,215 to 352,492
_RS22335
Position (kb) 349
350
351 Strain fitness (log2 ratio) -2
-1
0
1
2 at 348.354 kb on + strand, within RR42_RS22320 at 348.354 kb on + strand, within RR42_RS22320 at 348.354 kb on + strand, within RR42_RS22320 at 348.355 kb on - strand, within RR42_RS22320 at 348.355 kb on - strand, within RR42_RS22320 at 348.355 kb on - strand, within RR42_RS22320 at 348.685 kb on - strand, within RR42_RS22320 at 348.732 kb on + strand, within RR42_RS22320 at 348.750 kb on + strand, within RR42_RS22320 at 348.750 kb on + strand, within RR42_RS22320 at 348.750 kb on + strand, within RR42_RS22320 at 348.751 kb on - strand, within RR42_RS22320 at 348.808 kb on - strand, within RR42_RS22320 at 348.808 kb on - strand, within RR42_RS22320 at 348.939 kb on + strand, within RR42_RS22320 at 348.940 kb on - strand, within RR42_RS22320 at 349.044 kb on + strand at 349.045 kb on - strand at 349.056 kb on + strand at 349.056 kb on + strand at 349.056 kb on + strand at 349.056 kb on + strand at 349.056 kb on + strand at 349.056 kb on + strand at 349.057 kb on - strand at 349.057 kb on - strand at 349.068 kb on - strand at 349.355 kb on + strand, within RR42_RS22325 at 349.355 kb on + strand, within RR42_RS22325 at 349.355 kb on + strand, within RR42_RS22325 at 349.355 kb on + strand, within RR42_RS22325 at 349.356 kb on - strand, within RR42_RS22325 at 349.380 kb on + strand, within RR42_RS22325 at 349.465 kb on - strand, within RR42_RS22325 at 349.517 kb on + strand, within RR42_RS22325 at 349.647 kb on - strand, within RR42_RS22325 at 349.787 kb on + strand, within RR42_RS22325 at 349.787 kb on + strand, within RR42_RS22325 at 349.788 kb on - strand, within RR42_RS22325 at 349.788 kb on - strand, within RR42_RS22325 at 349.788 kb on - strand, within RR42_RS22325 at 349.797 kb on - strand, within RR42_RS22325 at 349.878 kb on + strand, within RR42_RS22325 at 349.879 kb on + strand, within RR42_RS22325 at 349.914 kb on - strand, within RR42_RS22325 at 350.051 kb on + strand, within RR42_RS22325 at 350.051 kb on + strand, within RR42_RS22325 at 350.051 kb on + strand, within RR42_RS22325 at 350.069 kb on + strand, within RR42_RS22325 at 350.070 kb on - strand, within RR42_RS22325 at 350.299 kb on - strand at 350.622 kb on + strand, within RR42_RS22330 at 350.622 kb on + strand, within RR42_RS22330 at 350.623 kb on - strand, within RR42_RS22330 at 350.623 kb on - strand, within RR42_RS22330 at 350.679 kb on + strand, within RR42_RS22330 at 350.679 kb on + strand, within RR42_RS22330 at 350.871 kb on + strand at 350.872 kb on - strand at 351.001 kb on - strand at 351.005 kb on - strand at 351.064 kb on + strand at 351.155 kb on + strand at 351.156 kb on - strand at 351.159 kb on + strand at 351.159 kb on + strand at 351.230 kb on + strand at 351.241 kb on + strand at 351.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 348,354 + RR42_RS22320 0.37 -0.9 348,354 + RR42_RS22320 0.37 +0.9 348,354 + RR42_RS22320 0.37 -0.5 348,355 - RR42_RS22320 0.37 -1.1 348,355 - RR42_RS22320 0.37 +0.5 348,355 - RR42_RS22320 0.37 +2.2 348,685 - RR42_RS22320 0.63 -0.5 348,732 + RR42_RS22320 0.66 +0.6 348,750 + RR42_RS22320 0.68 -1.4 348,750 + RR42_RS22320 0.68 -0.6 348,750 + RR42_RS22320 0.68 -0.2 348,751 - RR42_RS22320 0.68 -0.3 348,808 - RR42_RS22320 0.72 -0.8 348,808 - RR42_RS22320 0.72 -0.6 348,939 + RR42_RS22320 0.82 -0.5 348,940 - RR42_RS22320 0.82 +0.4 349,044 + +0.5 349,045 - -2.7 349,056 + -0.3 349,056 + +0.7 349,056 + -0.1 349,056 + -0.5 349,056 + -0.1 349,056 + +0.5 349,057 - +1.1 349,057 - +0.4 349,068 - +0.9 349,355 + RR42_RS22325 0.17 +0.7 349,355 + RR42_RS22325 0.17 +0.4 349,355 + RR42_RS22325 0.17 +0.6 349,355 + RR42_RS22325 0.17 +2.1 349,356 - RR42_RS22325 0.17 -0.0 349,380 + RR42_RS22325 0.19 +0.2 349,465 - RR42_RS22325 0.27 +0.1 349,517 + RR42_RS22325 0.32 +0.1 349,647 - RR42_RS22325 0.44 -0.1 349,787 + RR42_RS22325 0.56 +0.4 349,787 + RR42_RS22325 0.56 +0.3 349,788 - RR42_RS22325 0.56 +0.6 349,788 - RR42_RS22325 0.56 -1.2 349,788 - RR42_RS22325 0.56 +0.7 349,797 - RR42_RS22325 0.57 +0.0 349,878 + RR42_RS22325 0.65 +1.4 349,879 + RR42_RS22325 0.65 +0.0 349,914 - RR42_RS22325 0.68 -0.6 350,051 + RR42_RS22325 0.80 +0.6 350,051 + RR42_RS22325 0.80 -0.5 350,051 + RR42_RS22325 0.80 +0.5 350,069 + RR42_RS22325 0.82 -0.9 350,070 - RR42_RS22325 0.82 -0.0 350,299 - +0.6 350,622 + RR42_RS22330 0.64 -0.3 350,622 + RR42_RS22330 0.64 -0.3 350,623 - RR42_RS22330 0.65 -0.7 350,623 - RR42_RS22330 0.65 +0.9 350,679 + RR42_RS22330 0.76 +0.2 350,679 + RR42_RS22330 0.76 -0.1 350,871 + -0.5 350,872 - -0.1 351,001 - +1.9 351,005 - -0.4 351,064 + -1.0 351,155 + +0.1 351,156 - -0.2 351,159 + +0.2 351,159 + +0.2 351,230 + -0.9 351,241 + +0.2 351,242 - +0.3
Or see this region's nucleotide sequence