Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22275 and RR42_RS22280 are separated by 108 nucleotides RR42_RS22280 and RR42_RS22285 are separated by 72 nucleotides RR42_RS22285 and RR42_RS22290 are separated by 123 nucleotides
RR42_RS22275: RR42_RS22275 - hypothetical protein, at 339,648 to 339,914
_RS22275
RR42_RS22280: RR42_RS22280 - cupin, at 340,023 to 340,478
_RS22280
RR42_RS22285: RR42_RS22285 - methionine sulfoxide reductase A, at 340,551 to 341,291
_RS22285
RR42_RS22290: RR42_RS22290 - cytochrome C biogenesis protein, at 341,415 to 343,223
_RS22290
Position (kb) 340
341
342 Strain fitness (log2 ratio) -1
0
1
2 at 339.649 kb on + strand at 339.656 kb on + strand at 339.656 kb on + strand at 339.661 kb on + strand at 339.661 kb on + strand at 339.661 kb on + strand at 339.662 kb on - strand at 339.662 kb on - strand at 339.662 kb on - strand at 339.662 kb on - strand at 339.763 kb on + strand, within RR42_RS22275 at 339.764 kb on - strand, within RR42_RS22275 at 339.825 kb on + strand, within RR42_RS22275 at 339.932 kb on - strand at 339.967 kb on + strand at 339.967 kb on + strand at 339.968 kb on - strand at 340.083 kb on + strand, within RR42_RS22280 at 340.083 kb on + strand, within RR42_RS22280 at 340.120 kb on + strand, within RR42_RS22280 at 340.120 kb on + strand, within RR42_RS22280 at 340.120 kb on + strand, within RR42_RS22280 at 340.120 kb on + strand, within RR42_RS22280 at 340.121 kb on - strand, within RR42_RS22280 at 340.121 kb on - strand, within RR42_RS22280 at 340.121 kb on - strand, within RR42_RS22280 at 340.194 kb on + strand, within RR42_RS22280 at 340.194 kb on + strand, within RR42_RS22280 at 340.194 kb on + strand, within RR42_RS22280 at 340.194 kb on + strand, within RR42_RS22280 at 340.195 kb on - strand, within RR42_RS22280 at 340.195 kb on - strand, within RR42_RS22280 at 340.437 kb on + strand at 340.651 kb on + strand, within RR42_RS22285 at 340.651 kb on + strand, within RR42_RS22285 at 340.652 kb on - strand, within RR42_RS22285 at 340.690 kb on + strand, within RR42_RS22285 at 340.691 kb on - strand, within RR42_RS22285 at 340.779 kb on - strand, within RR42_RS22285 at 340.780 kb on + strand, within RR42_RS22285 at 340.780 kb on + strand, within RR42_RS22285 at 340.781 kb on - strand, within RR42_RS22285 at 340.781 kb on - strand, within RR42_RS22285 at 340.781 kb on - strand, within RR42_RS22285 at 340.835 kb on - strand, within RR42_RS22285 at 340.994 kb on - strand, within RR42_RS22285 at 341.322 kb on + strand at 341.323 kb on - strand at 341.355 kb on + strand at 341.435 kb on - strand at 341.435 kb on - strand at 341.513 kb on - strand at 341.776 kb on + strand, within RR42_RS22290 at 341.776 kb on + strand, within RR42_RS22290 at 341.776 kb on + strand, within RR42_RS22290 at 341.777 kb on - strand, within RR42_RS22290 at 341.777 kb on - strand, within RR42_RS22290 at 341.845 kb on + strand, within RR42_RS22290 at 341.845 kb on + strand, within RR42_RS22290 at 341.845 kb on + strand, within RR42_RS22290 at 342.001 kb on + strand, within RR42_RS22290 at 342.055 kb on + strand, within RR42_RS22290 at 342.100 kb on + strand, within RR42_RS22290 at 342.100 kb on + strand, within RR42_RS22290 at 342.100 kb on + strand, within RR42_RS22290 at 342.254 kb on - strand, within RR42_RS22290 at 342.254 kb on - strand, within RR42_RS22290 at 342.254 kb on - strand, within RR42_RS22290 at 342.254 kb on - strand, within RR42_RS22290 at 342.286 kb on + strand, within RR42_RS22290 at 342.287 kb on - strand, within RR42_RS22290
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 339,649 + +0.8 339,656 + +1.0 339,656 + -0.3 339,661 + -0.9 339,661 + +0.9 339,661 + -0.9 339,662 - +0.0 339,662 - -0.4 339,662 - -0.7 339,662 - +0.0 339,763 + RR42_RS22275 0.43 +0.3 339,764 - RR42_RS22275 0.43 +1.8 339,825 + RR42_RS22275 0.66 +0.2 339,932 - -0.1 339,967 + -0.9 339,967 + +0.5 339,968 - +0.1 340,083 + RR42_RS22280 0.13 +0.1 340,083 + RR42_RS22280 0.13 -1.3 340,120 + RR42_RS22280 0.21 -0.2 340,120 + RR42_RS22280 0.21 +0.6 340,120 + RR42_RS22280 0.21 -0.4 340,120 + RR42_RS22280 0.21 +1.4 340,121 - RR42_RS22280 0.21 +0.1 340,121 - RR42_RS22280 0.21 +0.5 340,121 - RR42_RS22280 0.21 +1.0 340,194 + RR42_RS22280 0.38 -0.5 340,194 + RR42_RS22280 0.38 +0.2 340,194 + RR42_RS22280 0.38 -0.3 340,194 + RR42_RS22280 0.38 +1.0 340,195 - RR42_RS22280 0.38 -1.6 340,195 - RR42_RS22280 0.38 +1.3 340,437 + -0.1 340,651 + RR42_RS22285 0.13 +0.2 340,651 + RR42_RS22285 0.13 +1.3 340,652 - RR42_RS22285 0.14 -0.4 340,690 + RR42_RS22285 0.19 -0.9 340,691 - RR42_RS22285 0.19 -0.3 340,779 - RR42_RS22285 0.31 -0.9 340,780 + RR42_RS22285 0.31 +0.2 340,780 + RR42_RS22285 0.31 -0.4 340,781 - RR42_RS22285 0.31 -1.0 340,781 - RR42_RS22285 0.31 -1.4 340,781 - RR42_RS22285 0.31 -0.8 340,835 - RR42_RS22285 0.38 +0.1 340,994 - RR42_RS22285 0.60 +0.3 341,322 + +0.5 341,323 - -0.3 341,355 + -0.9 341,435 - -1.1 341,435 - +0.4 341,513 - -1.2 341,776 + RR42_RS22290 0.20 +0.8 341,776 + RR42_RS22290 0.20 +0.3 341,776 + RR42_RS22290 0.20 -0.0 341,777 - RR42_RS22290 0.20 +0.4 341,777 - RR42_RS22290 0.20 -0.8 341,845 + RR42_RS22290 0.24 +0.3 341,845 + RR42_RS22290 0.24 +1.5 341,845 + RR42_RS22290 0.24 -0.6 342,001 + RR42_RS22290 0.32 +1.3 342,055 + RR42_RS22290 0.35 -0.3 342,100 + RR42_RS22290 0.38 -0.5 342,100 + RR42_RS22290 0.38 -0.3 342,100 + RR42_RS22290 0.38 -0.2 342,254 - RR42_RS22290 0.46 +0.9 342,254 - RR42_RS22290 0.46 -0.7 342,254 - RR42_RS22290 0.46 -0.1 342,254 - RR42_RS22290 0.46 -0.0 342,286 + RR42_RS22290 0.48 -0.0 342,287 - RR42_RS22290 0.48 -0.2
Or see this region's nucleotide sequence