Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21965 and RR42_RS21970 are separated by 923 nucleotides RR42_RS21970 and RR42_RS21975 overlap by 38 nucleotides
RR42_RS21965: RR42_RS21965 - hypothetical protein, at 253,337 to 254,548
_RS21965
RR42_RS21970: RR42_RS21970 - hypothetical protein, at 255,472 to 258,339
_RS21970
RR42_RS21975: RR42_RS21975 - hypothetical protein, at 258,302 to 262,522
_RS21975
Position (kb)
255
256
257
258
259 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 254.616 kb on + strand at 254.617 kb on - strand at 254.623 kb on + strand at 254.623 kb on + strand at 254.624 kb on - strand at 254.630 kb on + strand at 254.631 kb on - strand at 254.754 kb on + strand at 254.831 kb on + strand at 254.949 kb on + strand at 254.950 kb on - strand at 254.950 kb on - strand at 254.950 kb on - strand at 254.950 kb on - strand at 254.950 kb on - strand at 254.957 kb on + strand at 254.957 kb on + strand at 254.957 kb on + strand at 254.958 kb on - strand at 254.958 kb on - strand at 255.135 kb on + strand at 255.135 kb on + strand at 255.135 kb on + strand at 255.135 kb on + strand at 255.135 kb on + strand at 255.136 kb on - strand at 255.136 kb on - strand at 255.149 kb on + strand at 255.156 kb on - strand at 255.156 kb on - strand at 255.163 kb on + strand at 255.163 kb on + strand at 255.164 kb on - strand at 255.164 kb on - strand at 255.164 kb on - strand at 255.165 kb on + strand at 255.165 kb on + strand at 255.210 kb on + strand at 255.210 kb on + strand at 255.211 kb on - strand at 255.684 kb on + strand at 255.700 kb on + strand at 255.701 kb on - strand at 256.055 kb on - strand, within RR42_RS21970 at 256.174 kb on + strand, within RR42_RS21970 at 256.273 kb on + strand, within RR42_RS21970 at 256.274 kb on - strand, within RR42_RS21970 at 256.417 kb on + strand, within RR42_RS21970 at 256.417 kb on + strand, within RR42_RS21970 at 256.418 kb on - strand, within RR42_RS21970 at 256.418 kb on - strand, within RR42_RS21970 at 256.418 kb on - strand, within RR42_RS21970 at 256.427 kb on - strand, within RR42_RS21970 at 256.811 kb on - strand, within RR42_RS21970 at 256.847 kb on + strand, within RR42_RS21970 at 256.848 kb on - strand, within RR42_RS21970 at 256.848 kb on - strand, within RR42_RS21970 at 256.858 kb on + strand, within RR42_RS21970 at 257.143 kb on + strand, within RR42_RS21970 at 257.143 kb on + strand, within RR42_RS21970 at 257.143 kb on + strand, within RR42_RS21970 at 257.143 kb on + strand, within RR42_RS21970 at 257.144 kb on - strand, within RR42_RS21970 at 257.200 kb on + strand, within RR42_RS21970 at 257.201 kb on - strand, within RR42_RS21970 at 257.323 kb on + strand, within RR42_RS21970 at 257.367 kb on + strand, within RR42_RS21970 at 257.367 kb on + strand, within RR42_RS21970 at 257.368 kb on - strand, within RR42_RS21970 at 257.368 kb on - strand, within RR42_RS21970 at 257.497 kb on - strand, within RR42_RS21970 at 257.577 kb on - strand, within RR42_RS21970 at 257.577 kb on - strand, within RR42_RS21970 at 257.605 kb on + strand, within RR42_RS21970 at 257.605 kb on + strand, within RR42_RS21970 at 257.605 kb on + strand, within RR42_RS21970 at 257.605 kb on + strand, within RR42_RS21970 at 257.629 kb on + strand, within RR42_RS21970 at 257.633 kb on - strand, within RR42_RS21970 at 257.633 kb on - strand, within RR42_RS21970 at 257.704 kb on + strand, within RR42_RS21970 at 257.704 kb on + strand, within RR42_RS21970 at 257.704 kb on + strand, within RR42_RS21970 at 257.704 kb on + strand, within RR42_RS21970 at 257.705 kb on - strand, within RR42_RS21970 at 257.705 kb on - strand, within RR42_RS21970 at 257.707 kb on + strand, within RR42_RS21970 at 257.707 kb on + strand, within RR42_RS21970 at 257.717 kb on - strand, within RR42_RS21970 at 257.761 kb on + strand, within RR42_RS21970 at 257.767 kb on + strand, within RR42_RS21970 at 257.810 kb on - strand, within RR42_RS21970 at 257.810 kb on - strand, within RR42_RS21970 at 258.004 kb on + strand, within RR42_RS21970 at 258.005 kb on - strand, within RR42_RS21970 at 258.060 kb on + strand at 258.173 kb on - strand at 258.549 kb on - strand at 258.773 kb on + strand, within RR42_RS21975 at 258.773 kb on + strand, within RR42_RS21975 at 258.774 kb on - strand, within RR42_RS21975 at 258.774 kb on - strand, within RR42_RS21975 at 258.897 kb on - strand, within RR42_RS21975 at 258.897 kb on - strand, within RR42_RS21975 at 259.052 kb on + strand, within RR42_RS21975 at 259.052 kb on + strand, within RR42_RS21975 at 259.052 kb on + strand, within RR42_RS21975 at 259.211 kb on + strand, within RR42_RS21975 at 259.212 kb on - strand, within RR42_RS21975 at 259.212 kb on - strand, within RR42_RS21975
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 254,616 + -0.7 254,617 - -1.4 254,623 + -0.8 254,623 + -0.2 254,624 - -0.1 254,630 + +0.5 254,631 - +0.5 254,754 + +0.3 254,831 + -0.8 254,949 + +1.6 254,950 - +1.1 254,950 - +1.7 254,950 - -2.1 254,950 - +0.8 254,950 - -0.1 254,957 + +0.1 254,957 + -0.0 254,957 + +0.1 254,958 - -0.3 254,958 - -1.8 255,135 + -1.9 255,135 + +4.1 255,135 + +0.4 255,135 + +0.9 255,135 + -0.2 255,136 - +0.7 255,136 - +0.4 255,149 + +0.7 255,156 - +1.5 255,156 - +0.2 255,163 + +0.0 255,163 + +0.0 255,164 - +0.7 255,164 - -2.2 255,164 - -0.4 255,165 + +0.5 255,165 + +0.1 255,210 + -0.1 255,210 + +0.2 255,211 - -0.5 255,684 + +0.2 255,700 + -0.3 255,701 - +0.0 256,055 - RR42_RS21970 0.20 +1.4 256,174 + RR42_RS21970 0.24 +0.1 256,273 + RR42_RS21970 0.28 -0.7 256,274 - RR42_RS21970 0.28 -2.5 256,417 + RR42_RS21970 0.33 -0.4 256,417 + RR42_RS21970 0.33 -1.2 256,418 - RR42_RS21970 0.33 -0.7 256,418 - RR42_RS21970 0.33 -0.6 256,418 - RR42_RS21970 0.33 -0.3 256,427 - RR42_RS21970 0.33 +0.9 256,811 - RR42_RS21970 0.47 -0.1 256,847 + RR42_RS21970 0.48 -0.1 256,848 - RR42_RS21970 0.48 -1.8 256,848 - RR42_RS21970 0.48 -0.6 256,858 + RR42_RS21970 0.48 +0.1 257,143 + RR42_RS21970 0.58 -1.1 257,143 + RR42_RS21970 0.58 +0.7 257,143 + RR42_RS21970 0.58 -0.3 257,143 + RR42_RS21970 0.58 -0.3 257,144 - RR42_RS21970 0.58 -0.5 257,200 + RR42_RS21970 0.60 +0.6 257,201 - RR42_RS21970 0.60 -0.6 257,323 + RR42_RS21970 0.65 -1.5 257,367 + RR42_RS21970 0.66 -1.2 257,367 + RR42_RS21970 0.66 +0.2 257,368 - RR42_RS21970 0.66 -0.8 257,368 - RR42_RS21970 0.66 -0.1 257,497 - RR42_RS21970 0.71 -0.2 257,577 - RR42_RS21970 0.73 -0.9 257,577 - RR42_RS21970 0.73 -0.3 257,605 + RR42_RS21970 0.74 +0.3 257,605 + RR42_RS21970 0.74 +0.4 257,605 + RR42_RS21970 0.74 -0.4 257,605 + RR42_RS21970 0.74 +0.3 257,629 + RR42_RS21970 0.75 +1.5 257,633 - RR42_RS21970 0.75 +0.9 257,633 - RR42_RS21970 0.75 -0.7 257,704 + RR42_RS21970 0.78 +0.0 257,704 + RR42_RS21970 0.78 +0.1 257,704 + RR42_RS21970 0.78 -0.4 257,704 + RR42_RS21970 0.78 -0.8 257,705 - RR42_RS21970 0.78 -0.4 257,705 - RR42_RS21970 0.78 +0.4 257,707 + RR42_RS21970 0.78 -1.8 257,707 + RR42_RS21970 0.78 +1.2 257,717 - RR42_RS21970 0.78 +0.9 257,761 + RR42_RS21970 0.80 +1.0 257,767 + RR42_RS21970 0.80 -0.0 257,810 - RR42_RS21970 0.82 -0.2 257,810 - RR42_RS21970 0.82 -0.6 258,004 + RR42_RS21970 0.88 -0.9 258,005 - RR42_RS21970 0.88 +0.0 258,060 + +0.1 258,173 - +0.4 258,549 - -0.4 258,773 + RR42_RS21975 0.11 +0.5 258,773 + RR42_RS21975 0.11 -0.4 258,774 - RR42_RS21975 0.11 +0.8 258,774 - RR42_RS21975 0.11 -0.8 258,897 - RR42_RS21975 0.14 -0.2 258,897 - RR42_RS21975 0.14 +0.6 259,052 + RR42_RS21975 0.18 +0.1 259,052 + RR42_RS21975 0.18 -0.2 259,052 + RR42_RS21975 0.18 +0.1 259,211 + RR42_RS21975 0.22 +0.4 259,212 - RR42_RS21975 0.22 -0.4 259,212 - RR42_RS21975 0.22 -1.6
Or see this region's nucleotide sequence