Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21815 and RR42_RS21820 are separated by 426 nucleotides RR42_RS21820 and RR42_RS21825 are separated by 89 nucleotides RR42_RS21825 and RR42_RS21830 are separated by 96 nucleotides
RR42_RS21815: RR42_RS21815 - amino acid-binding protein, at 222,852 to 224,099
_RS21815
RR42_RS21820: RR42_RS21820 - GntR family transcriptional regulator, at 224,526 to 225,230
_RS21820
RR42_RS21825: RR42_RS21825 - hypothetical protein, at 225,320 to 225,868
_RS21825
RR42_RS21830: RR42_RS21830 - transcriptional regulator, at 225,965 to 226,879
_RS21830
Position (kb) 224
225
226 Strain fitness (log2 ratio) -1
0
1
2 at 223.555 kb on + strand, within RR42_RS21815 at 223.555 kb on + strand, within RR42_RS21815 at 223.663 kb on + strand, within RR42_RS21815 at 223.663 kb on + strand, within RR42_RS21815 at 223.879 kb on + strand, within RR42_RS21815 at 223.880 kb on - strand, within RR42_RS21815 at 223.880 kb on - strand, within RR42_RS21815 at 224.114 kb on + strand at 224.253 kb on - strand at 224.300 kb on + strand at 224.302 kb on + strand at 224.322 kb on + strand at 224.323 kb on - strand at 224.396 kb on + strand at 224.396 kb on + strand at 224.420 kb on - strand at 224.464 kb on + strand at 224.922 kb on + strand, within RR42_RS21820 at 225.072 kb on - strand, within RR42_RS21820 at 225.072 kb on - strand, within RR42_RS21820 at 225.129 kb on + strand, within RR42_RS21820 at 225.216 kb on + strand at 225.216 kb on + strand at 225.216 kb on + strand at 225.217 kb on - strand at 225.217 kb on - strand at 225.296 kb on + strand at 225.321 kb on + strand at 225.322 kb on - strand at 225.584 kb on - strand, within RR42_RS21825 at 225.709 kb on + strand, within RR42_RS21825 at 225.710 kb on - strand, within RR42_RS21825 at 225.710 kb on - strand, within RR42_RS21825 at 225.897 kb on + strand at 225.897 kb on + strand at 226.040 kb on + strand at 226.066 kb on - strand, within RR42_RS21830 at 226.066 kb on - strand, within RR42_RS21830
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 223,555 + RR42_RS21815 0.56 -0.5 223,555 + RR42_RS21815 0.56 -0.5 223,663 + RR42_RS21815 0.65 +0.5 223,663 + RR42_RS21815 0.65 -0.5 223,879 + RR42_RS21815 0.82 -0.2 223,880 - RR42_RS21815 0.82 +0.4 223,880 - RR42_RS21815 0.82 +0.8 224,114 + -0.5 224,253 - -0.1 224,300 + +0.1 224,302 + +0.5 224,322 + -0.1 224,323 - -0.4 224,396 + +0.1 224,396 + -0.9 224,420 - -1.5 224,464 + +0.1 224,922 + RR42_RS21820 0.56 +0.5 225,072 - RR42_RS21820 0.77 +0.6 225,072 - RR42_RS21820 0.77 +0.8 225,129 + RR42_RS21820 0.86 +0.2 225,216 + -0.1 225,216 + -0.5 225,216 + +0.0 225,217 - +0.1 225,217 - +1.5 225,296 + -0.2 225,321 + +0.1 225,322 - -0.6 225,584 - RR42_RS21825 0.48 +0.1 225,709 + RR42_RS21825 0.71 -0.7 225,710 - RR42_RS21825 0.71 -1.3 225,710 - RR42_RS21825 0.71 +0.0 225,897 + +1.9 225,897 + +1.0 226,040 + +0.2 226,066 - RR42_RS21830 0.11 -0.5 226,066 - RR42_RS21830 0.11 +1.2
Or see this region's nucleotide sequence