Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21730 and RR42_RS21735 overlap by 1 nucleotides RR42_RS21735 and RR42_RS21740 are separated by 131 nucleotides RR42_RS21740 and RR42_RS21745 are separated by 65 nucleotides
RR42_RS21730: RR42_RS21730 - pyruvate dehydrogenase subunit beta, at 203,953 to 204,969
_RS21730
RR42_RS21735: RR42_RS21735 - acetoin dehydrogenase, at 204,969 to 206,081
_RS21735
RR42_RS21740: RR42_RS21740 - acetoin dehydrogenase, at 206,213 to 206,989
_RS21740
RR42_RS21745: RR42_RS21745 - hypothetical protein, at 207,055 to 207,810
_RS21745
Position (kb) 204
205
206
207 Strain fitness (log2 ratio) -2
-1
0
1
2 at 204.118 kb on + strand, within RR42_RS21730 at 204.119 kb on - strand, within RR42_RS21730 at 204.169 kb on + strand, within RR42_RS21730 at 204.169 kb on + strand, within RR42_RS21730 at 204.170 kb on - strand, within RR42_RS21730 at 204.289 kb on + strand, within RR42_RS21730 at 204.290 kb on - strand, within RR42_RS21730 at 204.400 kb on - strand, within RR42_RS21730 at 204.436 kb on + strand, within RR42_RS21730 at 204.436 kb on + strand, within RR42_RS21730 at 204.436 kb on + strand, within RR42_RS21730 at 204.544 kb on + strand, within RR42_RS21730 at 204.904 kb on + strand at 204.904 kb on + strand at 204.904 kb on + strand at 204.905 kb on - strand at 204.905 kb on - strand at 205.302 kb on + strand, within RR42_RS21735 at 205.302 kb on + strand, within RR42_RS21735 at 205.302 kb on + strand, within RR42_RS21735 at 205.339 kb on - strand, within RR42_RS21735 at 205.503 kb on - strand, within RR42_RS21735 at 205.689 kb on + strand, within RR42_RS21735 at 205.689 kb on + strand, within RR42_RS21735 at 205.803 kb on + strand, within RR42_RS21735 at 205.803 kb on + strand, within RR42_RS21735 at 206.018 kb on + strand at 206.158 kb on + strand at 206.158 kb on + strand at 206.158 kb on + strand at 206.159 kb on - strand at 206.171 kb on + strand at 206.171 kb on + strand at 206.172 kb on - strand at 206.172 kb on - strand at 206.172 kb on - strand at 206.201 kb on - strand at 206.678 kb on + strand, within RR42_RS21740 at 206.678 kb on + strand, within RR42_RS21740 at 206.786 kb on + strand, within RR42_RS21740 at 206.786 kb on + strand, within RR42_RS21740 at 206.786 kb on + strand, within RR42_RS21740 at 206.786 kb on + strand, within RR42_RS21740 at 206.787 kb on - strand, within RR42_RS21740 at 206.787 kb on - strand, within RR42_RS21740 at 206.787 kb on - strand, within RR42_RS21740 at 206.788 kb on + strand, within RR42_RS21740 at 206.789 kb on - strand, within RR42_RS21740 at 206.789 kb on - strand, within RR42_RS21740 at 206.789 kb on - strand, within RR42_RS21740 at 206.789 kb on - strand, within RR42_RS21740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 204,118 + RR42_RS21730 0.16 +0.4 204,119 - RR42_RS21730 0.16 +0.0 204,169 + RR42_RS21730 0.21 +0.3 204,169 + RR42_RS21730 0.21 -0.5 204,170 - RR42_RS21730 0.21 +0.5 204,289 + RR42_RS21730 0.33 -0.8 204,290 - RR42_RS21730 0.33 -2.6 204,400 - RR42_RS21730 0.44 -0.2 204,436 + RR42_RS21730 0.47 +1.1 204,436 + RR42_RS21730 0.47 -0.6 204,436 + RR42_RS21730 0.47 -0.1 204,544 + RR42_RS21730 0.58 -1.6 204,904 + +0.4 204,904 + +1.0 204,904 + +1.3 204,905 - +0.2 204,905 - +0.2 205,302 + RR42_RS21735 0.30 -2.7 205,302 + RR42_RS21735 0.30 -1.5 205,302 + RR42_RS21735 0.30 +0.7 205,339 - RR42_RS21735 0.33 -0.4 205,503 - RR42_RS21735 0.48 +0.2 205,689 + RR42_RS21735 0.65 -0.0 205,689 + RR42_RS21735 0.65 -0.3 205,803 + RR42_RS21735 0.75 +0.6 205,803 + RR42_RS21735 0.75 +0.7 206,018 + -0.1 206,158 + -0.2 206,158 + -0.0 206,158 + -0.4 206,159 - -0.4 206,171 + -0.0 206,171 + +0.1 206,172 - +0.1 206,172 - -0.1 206,172 - -0.6 206,201 - +0.4 206,678 + RR42_RS21740 0.60 +0.5 206,678 + RR42_RS21740 0.60 -0.7 206,786 + RR42_RS21740 0.74 -1.0 206,786 + RR42_RS21740 0.74 +0.3 206,786 + RR42_RS21740 0.74 +0.2 206,786 + RR42_RS21740 0.74 +1.8 206,787 - RR42_RS21740 0.74 +0.3 206,787 - RR42_RS21740 0.74 -0.5 206,787 - RR42_RS21740 0.74 +0.4 206,788 + RR42_RS21740 0.74 -1.2 206,789 - RR42_RS21740 0.74 +0.4 206,789 - RR42_RS21740 0.74 +0.5 206,789 - RR42_RS21740 0.74 -0.2 206,789 - RR42_RS21740 0.74 -1.0
Or see this region's nucleotide sequence