Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19835 and RR42_RS19840 are separated by 55 nucleotides RR42_RS19840 and RR42_RS19845 are separated by 112 nucleotides
RR42_RS19835: RR42_RS19835 - MFS transporter, at 4,280,897 to 4,282,210
_RS19835
RR42_RS19840: RR42_RS19840 - amidohydrolase, at 4,282,266 to 4,283,477
_RS19840
RR42_RS19845: RR42_RS19845 - hypothetical protein, at 4,283,590 to 4,284,513
_RS19845
Position (kb) 4282
4283
4284 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4281.467 kb on + strand, within RR42_RS19835 at 4281.467 kb on + strand, within RR42_RS19835 at 4281.468 kb on - strand, within RR42_RS19835 at 4281.554 kb on + strand, within RR42_RS19835 at 4281.554 kb on + strand, within RR42_RS19835 at 4281.554 kb on + strand, within RR42_RS19835 at 4281.554 kb on + strand, within RR42_RS19835 at 4281.554 kb on + strand, within RR42_RS19835 at 4281.554 kb on + strand, within RR42_RS19835 at 4281.555 kb on - strand, within RR42_RS19835 at 4281.689 kb on + strand, within RR42_RS19835 at 4281.701 kb on + strand, within RR42_RS19835 at 4281.702 kb on - strand, within RR42_RS19835 at 4281.702 kb on - strand, within RR42_RS19835 at 4281.716 kb on + strand, within RR42_RS19835 at 4281.884 kb on + strand, within RR42_RS19835 at 4281.884 kb on + strand, within RR42_RS19835 at 4282.046 kb on + strand, within RR42_RS19835 at 4282.047 kb on - strand, within RR42_RS19835 at 4282.047 kb on - strand, within RR42_RS19835 at 4282.047 kb on - strand, within RR42_RS19835 at 4282.047 kb on - strand, within RR42_RS19835 at 4282.139 kb on + strand at 4282.139 kb on + strand at 4282.260 kb on - strand at 4282.260 kb on - strand at 4282.443 kb on + strand, within RR42_RS19840 at 4282.794 kb on - strand, within RR42_RS19840 at 4283.016 kb on - strand, within RR42_RS19840 at 4283.016 kb on - strand, within RR42_RS19840 at 4283.340 kb on + strand, within RR42_RS19840 at 4283.341 kb on - strand, within RR42_RS19840 at 4283.401 kb on - strand at 4283.464 kb on - strand at 4283.476 kb on - strand at 4283.591 kb on + strand at 4283.591 kb on + strand at 4283.591 kb on + strand at 4283.855 kb on + strand, within RR42_RS19845 at 4283.856 kb on - strand, within RR42_RS19845 at 4283.936 kb on + strand, within RR42_RS19845 at 4284.068 kb on - strand, within RR42_RS19845 at 4284.068 kb on - strand, within RR42_RS19845 at 4284.068 kb on - strand, within RR42_RS19845 at 4284.275 kb on + strand, within RR42_RS19845 at 4284.276 kb on - strand, within RR42_RS19845 at 4284.276 kb on - strand, within RR42_RS19845 at 4284.321 kb on - strand, within RR42_RS19845 at 4284.321 kb on - strand, within RR42_RS19845 at 4284.330 kb on - strand, within RR42_RS19845 at 4284.330 kb on - strand, within RR42_RS19845 at 4284.350 kb on - strand, within RR42_RS19845
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 4,281,467 + RR42_RS19835 0.43 -0.1 4,281,467 + RR42_RS19835 0.43 -0.3 4,281,468 - RR42_RS19835 0.43 -0.5 4,281,554 + RR42_RS19835 0.50 +0.8 4,281,554 + RR42_RS19835 0.50 -0.2 4,281,554 + RR42_RS19835 0.50 +0.4 4,281,554 + RR42_RS19835 0.50 +0.6 4,281,554 + RR42_RS19835 0.50 -0.2 4,281,554 + RR42_RS19835 0.50 -2.2 4,281,555 - RR42_RS19835 0.50 +0.5 4,281,689 + RR42_RS19835 0.60 -0.3 4,281,701 + RR42_RS19835 0.61 +0.4 4,281,702 - RR42_RS19835 0.61 -0.5 4,281,702 - RR42_RS19835 0.61 -1.4 4,281,716 + RR42_RS19835 0.62 -0.2 4,281,884 + RR42_RS19835 0.75 -0.6 4,281,884 + RR42_RS19835 0.75 -0.8 4,282,046 + RR42_RS19835 0.87 +0.2 4,282,047 - RR42_RS19835 0.88 +1.1 4,282,047 - RR42_RS19835 0.88 +3.4 4,282,047 - RR42_RS19835 0.88 -0.3 4,282,047 - RR42_RS19835 0.88 -1.8 4,282,139 + +0.5 4,282,139 + +0.2 4,282,260 - -0.9 4,282,260 - +0.2 4,282,443 + RR42_RS19840 0.15 +0.8 4,282,794 - RR42_RS19840 0.44 +0.1 4,283,016 - RR42_RS19840 0.62 +0.5 4,283,016 - RR42_RS19840 0.62 -1.0 4,283,340 + RR42_RS19840 0.89 -0.0 4,283,341 - RR42_RS19840 0.89 -0.5 4,283,401 - -0.7 4,283,464 - -0.4 4,283,476 - -1.1 4,283,591 + +1.4 4,283,591 + -0.5 4,283,591 + -0.8 4,283,855 + RR42_RS19845 0.29 -0.8 4,283,856 - RR42_RS19845 0.29 +0.1 4,283,936 + RR42_RS19845 0.37 -1.9 4,284,068 - RR42_RS19845 0.52 -0.8 4,284,068 - RR42_RS19845 0.52 -1.7 4,284,068 - RR42_RS19845 0.52 +0.2 4,284,275 + RR42_RS19845 0.74 -1.6 4,284,276 - RR42_RS19845 0.74 -0.6 4,284,276 - RR42_RS19845 0.74 -0.5 4,284,321 - RR42_RS19845 0.79 -0.1 4,284,321 - RR42_RS19845 0.79 +0.1 4,284,330 - RR42_RS19845 0.80 -0.2 4,284,330 - RR42_RS19845 0.80 -3.0 4,284,350 - RR42_RS19845 0.82 -1.7
Or see this region's nucleotide sequence