Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS19270

Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS19265 and RR42_RS19270 are separated by 71 nucleotidesRR42_RS19270 and RR42_RS19275 are separated by 112 nucleotides RR42_RS19265: RR42_RS19265 - alanine-phosphoribitol ligase, at 4,158,901 to 4,160,142 _RS19265 RR42_RS19270: RR42_RS19270 - short-chain dehydrogenase, at 4,160,214 to 4,161,002 _RS19270 RR42_RS19275: RR42_RS19275 - carboxymethylenebutenolidase, at 4,161,115 to 4,162,344 _RS19275 Position (kb) 4160 4161 4162Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4159.249 kb on + strand, within RR42_RS19265at 4159.250 kb on - strand, within RR42_RS19265at 4159.250 kb on - strand, within RR42_RS19265at 4159.402 kb on - strand, within RR42_RS19265at 4159.402 kb on - strand, within RR42_RS19265at 4159.402 kb on - strand, within RR42_RS19265at 4159.402 kb on - strand, within RR42_RS19265at 4159.444 kb on - strand, within RR42_RS19265at 4159.541 kb on + strand, within RR42_RS19265at 4159.613 kb on + strand, within RR42_RS19265at 4159.613 kb on + strand, within RR42_RS19265at 4159.613 kb on + strand, within RR42_RS19265at 4159.613 kb on + strand, within RR42_RS19265at 4159.613 kb on + strand, within RR42_RS19265at 4159.613 kb on + strand, within RR42_RS19265at 4159.613 kb on + strand, within RR42_RS19265at 4159.614 kb on - strand, within RR42_RS19265at 4159.614 kb on - strand, within RR42_RS19265at 4159.614 kb on - strand, within RR42_RS19265at 4159.614 kb on - strand, within RR42_RS19265at 4159.614 kb on - strand, within RR42_RS19265at 4159.614 kb on - strand, within RR42_RS19265at 4159.614 kb on - strand, within RR42_RS19265at 4159.924 kb on + strand, within RR42_RS19265at 4159.924 kb on + strand, within RR42_RS19265at 4159.924 kb on + strand, within RR42_RS19265at 4159.925 kb on - strand, within RR42_RS19265at 4159.925 kb on - strand, within RR42_RS19265at 4159.938 kb on + strand, within RR42_RS19265at 4159.938 kb on + strand, within RR42_RS19265at 4159.939 kb on - strand, within RR42_RS19265at 4159.939 kb on - strand, within RR42_RS19265at 4159.939 kb on - strand, within RR42_RS19265at 4159.939 kb on - strand, within RR42_RS19265at 4159.939 kb on - strand, within RR42_RS19265at 4159.939 kb on - strand, within RR42_RS19265at 4159.939 kb on - strand, within RR42_RS19265at 4159.939 kb on - strand, within RR42_RS19265at 4160.066 kb on + strandat 4160.067 kb on - strandat 4160.067 kb on - strandat 4160.153 kb on + strandat 4160.153 kb on + strandat 4160.153 kb on + strandat 4160.154 kb on - strandat 4160.154 kb on - strandat 4160.154 kb on - strandat 4160.154 kb on - strandat 4160.156 kb on + strandat 4160.156 kb on + strandat 4160.156 kb on + strandat 4160.156 kb on + strandat 4160.156 kb on + strandat 4160.156 kb on + strandat 4160.157 kb on - strandat 4160.157 kb on - strandat 4160.157 kb on - strandat 4160.157 kb on - strandat 4160.157 kb on - strandat 4160.157 kb on - strandat 4160.159 kb on + strandat 4160.159 kb on + strandat 4160.159 kb on + strandat 4160.159 kb on + strandat 4160.159 kb on + strandat 4160.159 kb on + strandat 4160.160 kb on - strandat 4160.160 kb on - strandat 4160.160 kb on - strandat 4160.160 kb on - strandat 4160.160 kb on - strandat 4160.160 kb on - strandat 4160.160 kb on - strandat 4160.270 kb on - strandat 4160.287 kb on + strandat 4160.288 kb on - strandat 4160.288 kb on - strandat 4160.288 kb on - strandat 4160.288 kb on - strandat 4160.519 kb on + strand, within RR42_RS19270at 4160.520 kb on - strand, within RR42_RS19270at 4160.520 kb on - strand, within RR42_RS19270at 4160.551 kb on + strand, within RR42_RS19270at 4160.588 kb on + strand, within RR42_RS19270at 4160.588 kb on + strand, within RR42_RS19270at 4160.588 kb on + strand, within RR42_RS19270at 4160.588 kb on + strand, within RR42_RS19270at 4160.588 kb on + strand, within RR42_RS19270at 4160.589 kb on - strand, within RR42_RS19270at 4160.589 kb on - strand, within RR42_RS19270at 4160.589 kb on - strand, within RR42_RS19270at 4160.731 kb on + strand, within RR42_RS19270at 4160.731 kb on + strand, within RR42_RS19270at 4160.731 kb on + strand, within RR42_RS19270at 4160.731 kb on + strand, within RR42_RS19270at 4160.731 kb on + strand, within RR42_RS19270at 4160.732 kb on - strand, within RR42_RS19270at 4160.755 kb on + strand, within RR42_RS19270at 4160.755 kb on + strand, within RR42_RS19270at 4160.756 kb on - strand, within RR42_RS19270at 4160.756 kb on - strand, within RR42_RS19270at 4160.756 kb on - strand, within RR42_RS19270at 4160.828 kb on + strand, within RR42_RS19270at 4161.086 kb on + strandat 4161.086 kb on + strandat 4161.086 kb on + strandat 4161.087 kb on - strandat 4161.087 kb on - strandat 4161.087 kb on - strandat 4161.096 kb on + strandat 4161.096 kb on + strandat 4161.096 kb on + strandat 4161.097 kb on - strandat 4161.097 kb on - strandat 4161.116 kb on + strandat 4161.116 kb on + strandat 4161.116 kb on + strandat 4161.116 kb on + strandat 4161.116 kb on + strandat 4161.116 kb on + strandat 4161.116 kb on + strandat 4161.117 kb on - strandat 4161.117 kb on - strandat 4161.266 kb on + strand, within RR42_RS19275at 4161.266 kb on + strand, within RR42_RS19275at 4161.266 kb on + strand, within RR42_RS19275at 4161.266 kb on + strand, within RR42_RS19275at 4161.266 kb on + strand, within RR42_RS19275at 4161.267 kb on - strand, within RR42_RS19275at 4161.267 kb on - strand, within RR42_RS19275at 4161.281 kb on + strand, within RR42_RS19275at 4161.281 kb on + strand, within RR42_RS19275at 4161.282 kb on - strand, within RR42_RS19275at 4161.282 kb on - strand, within RR42_RS19275at 4161.282 kb on - strand, within RR42_RS19275at 4161.381 kb on + strand, within RR42_RS19275at 4161.381 kb on + strand, within RR42_RS19275at 4161.382 kb on - strand, within RR42_RS19275at 4161.382 kb on - strand, within RR42_RS19275at 4161.456 kb on + strand, within RR42_RS19275at 4161.491 kb on + strand, within RR42_RS19275at 4161.491 kb on + strand, within RR42_RS19275at 4161.491 kb on + strand, within RR42_RS19275at 4161.492 kb on - strand, within RR42_RS19275at 4161.492 kb on - strand, within RR42_RS19275at 4161.492 kb on - strand, within RR42_RS19275at 4161.540 kb on + strand, within RR42_RS19275at 4161.540 kb on + strand, within RR42_RS19275at 4161.551 kb on + strand, within RR42_RS19275at 4161.551 kb on + strand, within RR42_RS19275at 4161.551 kb on + strand, within RR42_RS19275at 4161.552 kb on - strand, within RR42_RS19275at 4161.552 kb on - strand, within RR42_RS19275at 4161.608 kb on + strand, within RR42_RS19275at 4161.608 kb on + strand, within RR42_RS19275at 4161.644 kb on + strand, within RR42_RS19275at 4161.645 kb on - strand, within RR42_RS19275at 4161.645 kb on - strand, within RR42_RS19275at 4161.767 kb on + strand, within RR42_RS19275at 4161.767 kb on + strand, within RR42_RS19275at 4161.767 kb on + strand, within RR42_RS19275at 4161.767 kb on + strand, within RR42_RS19275at 4161.767 kb on + strand, within RR42_RS19275at 4161.767 kb on + strand, within RR42_RS19275at 4161.767 kb on + strand, within RR42_RS19275at 4161.767 kb on + strand, within RR42_RS19275at 4161.768 kb on - strand, within RR42_RS19275at 4161.768 kb on - strand, within RR42_RS19275at 4161.768 kb on - strand, within RR42_RS19275at 4161.768 kb on - strand, within RR42_RS19275at 4161.768 kb on - strand, within RR42_RS19275at 4161.917 kb on + strand, within RR42_RS19275at 4161.917 kb on + strand, within RR42_RS19275at 4161.917 kb on + strand, within RR42_RS19275at 4161.917 kb on + strand, within RR42_RS19275at 4161.918 kb on - strand, within RR42_RS19275at 4161.918 kb on - strand, within RR42_RS19275at 4161.920 kb on + strand, within RR42_RS19275at 4161.921 kb on - strand, within RR42_RS19275at 4161.921 kb on - strand, within RR42_RS19275at 4161.965 kb on + strand, within RR42_RS19275at 4161.966 kb on - strand, within RR42_RS19275

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml
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4,159,249 + RR42_RS19265 0.28 +0.3
4,159,250 - RR42_RS19265 0.28 +0.5
4,159,250 - RR42_RS19265 0.28 -0.1
4,159,402 - RR42_RS19265 0.40 +0.2
4,159,402 - RR42_RS19265 0.40 +1.6
4,159,402 - RR42_RS19265 0.40 +0.8
4,159,402 - RR42_RS19265 0.40 +0.4
4,159,444 - RR42_RS19265 0.44 +0.7
4,159,541 + RR42_RS19265 0.52 +0.0
4,159,613 + RR42_RS19265 0.57 -0.1
4,159,613 + RR42_RS19265 0.57 +0.9
4,159,613 + RR42_RS19265 0.57 -0.5
4,159,613 + RR42_RS19265 0.57 -0.1
4,159,613 + RR42_RS19265 0.57 +0.8
4,159,613 + RR42_RS19265 0.57 +0.5
4,159,613 + RR42_RS19265 0.57 -0.4
4,159,614 - RR42_RS19265 0.57 -0.5
4,159,614 - RR42_RS19265 0.57 -0.5
4,159,614 - RR42_RS19265 0.57 +1.0
4,159,614 - RR42_RS19265 0.57 +0.5
4,159,614 - RR42_RS19265 0.57 -0.1
4,159,614 - RR42_RS19265 0.57 -0.3
4,159,614 - RR42_RS19265 0.57 +0.1
4,159,924 + RR42_RS19265 0.82 +0.7
4,159,924 + RR42_RS19265 0.82 -1.1
4,159,924 + RR42_RS19265 0.82 -0.5
4,159,925 - RR42_RS19265 0.82 +0.9
4,159,925 - RR42_RS19265 0.82 +0.7
4,159,938 + RR42_RS19265 0.83 -0.1
4,159,938 + RR42_RS19265 0.83 -0.9
4,159,939 - RR42_RS19265 0.84 +0.2
4,159,939 - RR42_RS19265 0.84 +0.7
4,159,939 - RR42_RS19265 0.84 -1.0
4,159,939 - RR42_RS19265 0.84 -2.0
4,159,939 - RR42_RS19265 0.84 +0.2
4,159,939 - RR42_RS19265 0.84 -0.6
4,159,939 - RR42_RS19265 0.84 +0.6
4,159,939 - RR42_RS19265 0.84 +0.9
4,160,066 + +0.3
4,160,067 - -0.2
4,160,067 - +0.5
4,160,153 + -0.6
4,160,153 + +0.8
4,160,153 + -1.1
4,160,154 - +1.3
4,160,154 - -1.8
4,160,154 - -2.5
4,160,154 - -1.0
4,160,156 + -0.4
4,160,156 + -0.5
4,160,156 + +0.5
4,160,156 + -0.0
4,160,156 + -0.5
4,160,156 + -0.1
4,160,157 - -0.6
4,160,157 - -0.3
4,160,157 - -0.5
4,160,157 - -0.5
4,160,157 - +0.1
4,160,157 - +0.7
4,160,159 + +0.1
4,160,159 + -0.1
4,160,159 + +0.5
4,160,159 + -0.3
4,160,159 + +0.0
4,160,159 + -0.5
4,160,160 - +0.3
4,160,160 - +0.4
4,160,160 - +0.5
4,160,160 - +0.2
4,160,160 - +0.7
4,160,160 - -0.5
4,160,160 - +0.0
4,160,270 - +1.5
4,160,287 + -0.2
4,160,288 - -0.3
4,160,288 - -0.4
4,160,288 - -1.1
4,160,288 - +0.2
4,160,519 + RR42_RS19270 0.39 +0.3
4,160,520 - RR42_RS19270 0.39 +0.0
4,160,520 - RR42_RS19270 0.39 +1.8
4,160,551 + RR42_RS19270 0.43 -0.3
4,160,588 + RR42_RS19270 0.47 -0.8
4,160,588 + RR42_RS19270 0.47 -0.3
4,160,588 + RR42_RS19270 0.47 -0.6
4,160,588 + RR42_RS19270 0.47 -0.7
4,160,588 + RR42_RS19270 0.47 -0.5
4,160,589 - RR42_RS19270 0.48 +1.3
4,160,589 - RR42_RS19270 0.48 -0.4
4,160,589 - RR42_RS19270 0.48 -0.7
4,160,731 + RR42_RS19270 0.66 +0.2
4,160,731 + RR42_RS19270 0.66 +0.2
4,160,731 + RR42_RS19270 0.66 +0.8
4,160,731 + RR42_RS19270 0.66 -0.5
4,160,731 + RR42_RS19270 0.66 -0.6
4,160,732 - RR42_RS19270 0.66 -1.0
4,160,755 + RR42_RS19270 0.69 +0.3
4,160,755 + RR42_RS19270 0.69 +0.2
4,160,756 - RR42_RS19270 0.69 +0.5
4,160,756 - RR42_RS19270 0.69 +0.1
4,160,756 - RR42_RS19270 0.69 +0.5
4,160,828 + RR42_RS19270 0.78 -0.4
4,161,086 + +0.0
4,161,086 + +0.0
4,161,086 + -1.6
4,161,087 - -1.0
4,161,087 - -1.2
4,161,087 - -0.7
4,161,096 + +0.0
4,161,096 + +0.1
4,161,096 + +0.4
4,161,097 - +0.3
4,161,097 - +0.7
4,161,116 + +0.7
4,161,116 + +0.2
4,161,116 + +1.0
4,161,116 + +0.0
4,161,116 + +1.8
4,161,116 + +0.1
4,161,116 + +0.7
4,161,117 - +3.3
4,161,117 - -0.1
4,161,266 + RR42_RS19275 0.12 -0.7
4,161,266 + RR42_RS19275 0.12 +0.9
4,161,266 + RR42_RS19275 0.12 -1.8
4,161,266 + RR42_RS19275 0.12 -0.1
4,161,266 + RR42_RS19275 0.12 +0.4
4,161,267 - RR42_RS19275 0.12 -0.6
4,161,267 - RR42_RS19275 0.12 -4.0
4,161,281 + RR42_RS19275 0.13 +1.1
4,161,281 + RR42_RS19275 0.13 -0.0
4,161,282 - RR42_RS19275 0.14 +1.3
4,161,282 - RR42_RS19275 0.14 -1.6
4,161,282 - RR42_RS19275 0.14 +0.2
4,161,381 + RR42_RS19275 0.22 +0.2
4,161,381 + RR42_RS19275 0.22 +2.0
4,161,382 - RR42_RS19275 0.22 -1.4
4,161,382 - RR42_RS19275 0.22 -0.6
4,161,456 + RR42_RS19275 0.28 -0.3
4,161,491 + RR42_RS19275 0.31 +0.1
4,161,491 + RR42_RS19275 0.31 -1.3
4,161,491 + RR42_RS19275 0.31 -0.7
4,161,492 - RR42_RS19275 0.31 +0.5
4,161,492 - RR42_RS19275 0.31 +1.3
4,161,492 - RR42_RS19275 0.31 +0.4
4,161,540 + RR42_RS19275 0.35 +0.4
4,161,540 + RR42_RS19275 0.35 -0.6
4,161,551 + RR42_RS19275 0.35 -0.2
4,161,551 + RR42_RS19275 0.35 -0.4
4,161,551 + RR42_RS19275 0.35 +0.3
4,161,552 - RR42_RS19275 0.36 +0.1
4,161,552 - RR42_RS19275 0.36 +0.6
4,161,608 + RR42_RS19275 0.40 -1.5
4,161,608 + RR42_RS19275 0.40 +1.3
4,161,644 + RR42_RS19275 0.43 +0.0
4,161,645 - RR42_RS19275 0.43 -0.3
4,161,645 - RR42_RS19275 0.43 +0.2
4,161,767 + RR42_RS19275 0.53 -0.4
4,161,767 + RR42_RS19275 0.53 -0.4
4,161,767 + RR42_RS19275 0.53 +0.8
4,161,767 + RR42_RS19275 0.53 +0.4
4,161,767 + RR42_RS19275 0.53 +0.2
4,161,767 + RR42_RS19275 0.53 -1.1
4,161,767 + RR42_RS19275 0.53 -0.5
4,161,767 + RR42_RS19275 0.53 +0.9
4,161,768 - RR42_RS19275 0.53 +0.6
4,161,768 - RR42_RS19275 0.53 +0.4
4,161,768 - RR42_RS19275 0.53 -1.3
4,161,768 - RR42_RS19275 0.53 -0.7
4,161,768 - RR42_RS19275 0.53 +0.3
4,161,917 + RR42_RS19275 0.65 +1.8
4,161,917 + RR42_RS19275 0.65 -0.8
4,161,917 + RR42_RS19275 0.65 +0.3
4,161,917 + RR42_RS19275 0.65 -0.2
4,161,918 - RR42_RS19275 0.65 +0.2
4,161,918 - RR42_RS19275 0.65 -2.5
4,161,920 + RR42_RS19275 0.65 +1.1
4,161,921 - RR42_RS19275 0.66 -2.0
4,161,921 - RR42_RS19275 0.66 -1.3
4,161,965 + RR42_RS19275 0.69 -1.4
4,161,966 - RR42_RS19275 0.69 +0.8

Or see this region's nucleotide sequence