Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19250 and RR42_RS19255 are separated by 11 nucleotides RR42_RS19255 and RR42_RS19260 are separated by 236 nucleotides RR42_RS19260 and RR42_RS19265 are separated by 1 nucleotides
RR42_RS19250: RR42_RS19250 - 50S ribosomal protein L4, at 4,156,889 to 4,157,509
_RS19250
RR42_RS19255: RR42_RS19255 - 50S ribosomal protein L3, at 4,157,521 to 4,158,171
_RS19255
RR42_RS19260: RR42_RS19260 - nitrilotriacetate monooxygenase, at 4,158,408 to 4,158,899
_RS19260
RR42_RS19265: RR42_RS19265 - alanine-phosphoribitol ligase, at 4,158,901 to 4,160,142
_RS19265
Position (kb)
4158
4159 Strain fitness (log2 ratio)
-1
0
1
2 at 4158.358 kb on + strand at 4158.358 kb on + strand at 4158.358 kb on + strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.409 kb on + strand at 4158.565 kb on + strand, within RR42_RS19260 at 4158.565 kb on + strand, within RR42_RS19260 at 4158.566 kb on - strand, within RR42_RS19260 at 4158.566 kb on - strand, within RR42_RS19260 at 4159.118 kb on + strand, within RR42_RS19265 at 4159.118 kb on + strand, within RR42_RS19265 at 4159.119 kb on - strand, within RR42_RS19265 at 4159.249 kb on + strand, within RR42_RS19265 at 4159.250 kb on - strand, within RR42_RS19265 at 4159.250 kb on - strand, within RR42_RS19265 at 4159.402 kb on - strand, within RR42_RS19265 at 4159.402 kb on - strand, within RR42_RS19265 at 4159.402 kb on - strand, within RR42_RS19265 at 4159.402 kb on - strand, within RR42_RS19265 at 4159.444 kb on - strand, within RR42_RS19265 at 4159.541 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.613 kb on + strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265 at 4159.614 kb on - strand, within RR42_RS19265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 4,158,358 + +2.7 4,158,358 + +2.4 4,158,358 + +2.7 4,158,359 - +0.9 4,158,359 - -1.0 4,158,359 - +1.1 4,158,359 - +0.4 4,158,409 + +1.7 4,158,565 + RR42_RS19260 0.32 +0.4 4,158,565 + RR42_RS19260 0.32 +1.1 4,158,566 - RR42_RS19260 0.32 +0.6 4,158,566 - RR42_RS19260 0.32 +0.2 4,159,118 + RR42_RS19265 0.17 +0.1 4,159,118 + RR42_RS19265 0.17 -0.2 4,159,119 - RR42_RS19265 0.18 +1.3 4,159,249 + RR42_RS19265 0.28 +0.3 4,159,250 - RR42_RS19265 0.28 +0.5 4,159,250 - RR42_RS19265 0.28 -0.1 4,159,402 - RR42_RS19265 0.40 +0.2 4,159,402 - RR42_RS19265 0.40 +1.6 4,159,402 - RR42_RS19265 0.40 +0.8 4,159,402 - RR42_RS19265 0.40 +0.4 4,159,444 - RR42_RS19265 0.44 +0.7 4,159,541 + RR42_RS19265 0.52 +0.0 4,159,613 + RR42_RS19265 0.57 -0.1 4,159,613 + RR42_RS19265 0.57 +0.9 4,159,613 + RR42_RS19265 0.57 -0.5 4,159,613 + RR42_RS19265 0.57 -0.1 4,159,613 + RR42_RS19265 0.57 +0.8 4,159,613 + RR42_RS19265 0.57 +0.5 4,159,613 + RR42_RS19265 0.57 -0.4 4,159,614 - RR42_RS19265 0.57 -0.5 4,159,614 - RR42_RS19265 0.57 -0.5 4,159,614 - RR42_RS19265 0.57 +1.0 4,159,614 - RR42_RS19265 0.57 +0.5 4,159,614 - RR42_RS19265 0.57 -0.1 4,159,614 - RR42_RS19265 0.57 -0.3 4,159,614 - RR42_RS19265 0.57 +0.1
Or see this region's nucleotide sequence