Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18505 and RR42_RS18510 are separated by 179 nucleotides RR42_RS18510 and RR42_RS18515 are separated by 102 nucleotides RR42_RS18515 and RR42_RS18520 are separated by 50 nucleotides
RR42_RS18505: RR42_RS18505 - saccharopine dehydrogenase, at 4,004,723 to 4,005,928
_RS18505
RR42_RS18510: RR42_RS18510 - AsnC family transcriptional regulator, at 4,006,108 to 4,006,557
_RS18510
RR42_RS18515: RR42_RS18515 - dihydrodipicolinate reductase, at 4,006,660 to 4,007,469
_RS18515
RR42_RS18520: RR42_RS18520 - hypothetical protein, at 4,007,520 to 4,008,188
_RS18520
Position (kb)
4006
4007 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4005.153 kb on + strand, within RR42_RS18505 at 4005.153 kb on + strand, within RR42_RS18505 at 4005.215 kb on - strand, within RR42_RS18505 at 4005.217 kb on + strand, within RR42_RS18505 at 4005.218 kb on - strand, within RR42_RS18505 at 4005.218 kb on - strand, within RR42_RS18505 at 4005.218 kb on - strand, within RR42_RS18505 at 4005.229 kb on - strand, within RR42_RS18505 at 4005.321 kb on + strand, within RR42_RS18505 at 4005.322 kb on - strand, within RR42_RS18505 at 4005.322 kb on - strand, within RR42_RS18505 at 4005.322 kb on - strand, within RR42_RS18505 at 4005.360 kb on + strand, within RR42_RS18505 at 4005.361 kb on - strand, within RR42_RS18505 at 4005.361 kb on - strand, within RR42_RS18505 at 4005.361 kb on - strand, within RR42_RS18505 at 4005.475 kb on + strand, within RR42_RS18505 at 4005.580 kb on - strand, within RR42_RS18505 at 4005.580 kb on - strand, within RR42_RS18505 at 4005.580 kb on - strand, within RR42_RS18505 at 4005.967 kb on + strand at 4005.968 kb on - strand at 4005.968 kb on - strand at 4005.976 kb on + strand at 4005.976 kb on + strand at 4005.976 kb on + strand at 4005.977 kb on - strand at 4005.977 kb on - strand at 4005.977 kb on - strand at 4006.041 kb on + strand at 4006.042 kb on - strand at 4006.353 kb on - strand, within RR42_RS18510 at 4006.417 kb on + strand, within RR42_RS18510 at 4006.417 kb on + strand, within RR42_RS18510 at 4006.417 kb on + strand, within RR42_RS18510 at 4006.418 kb on - strand, within RR42_RS18510 at 4006.418 kb on - strand, within RR42_RS18510 at 4006.581 kb on + strand at 4006.582 kb on - strand at 4006.582 kb on - strand at 4006.612 kb on + strand at 4006.612 kb on + strand at 4006.613 kb on - strand at 4006.613 kb on - strand at 4006.613 kb on - strand at 4006.613 kb on - strand at 4006.613 kb on - strand at 4006.613 kb on - strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.616 kb on + strand at 4006.617 kb on - strand at 4006.617 kb on - strand at 4006.876 kb on + strand, within RR42_RS18515 at 4006.876 kb on + strand, within RR42_RS18515 at 4006.876 kb on + strand, within RR42_RS18515 at 4006.876 kb on + strand, within RR42_RS18515 at 4006.885 kb on + strand, within RR42_RS18515 at 4006.886 kb on - strand, within RR42_RS18515 at 4006.981 kb on + strand, within RR42_RS18515 at 4006.982 kb on - strand, within RR42_RS18515 at 4007.120 kb on - strand, within RR42_RS18515 at 4007.297 kb on - strand, within RR42_RS18515 at 4007.380 kb on + strand, within RR42_RS18515 at 4007.380 kb on + strand, within RR42_RS18515 at 4007.380 kb on + strand, within RR42_RS18515 at 4007.380 kb on + strand, within RR42_RS18515 at 4007.380 kb on + strand, within RR42_RS18515 at 4007.380 kb on + strand, within RR42_RS18515 at 4007.381 kb on - strand, within RR42_RS18515 at 4007.381 kb on - strand, within RR42_RS18515 at 4007.381 kb on - strand, within RR42_RS18515 at 4007.381 kb on - strand, within RR42_RS18515 at 4007.381 kb on - strand, within RR42_RS18515 at 4007.381 kb on - strand, within RR42_RS18515 at 4007.522 kb on - strand at 4007.522 kb on - strand at 4007.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 4,005,153 + RR42_RS18505 0.36 -0.3 4,005,153 + RR42_RS18505 0.36 +0.0 4,005,215 - RR42_RS18505 0.41 +0.3 4,005,217 + RR42_RS18505 0.41 +1.1 4,005,218 - RR42_RS18505 0.41 +0.5 4,005,218 - RR42_RS18505 0.41 -0.4 4,005,218 - RR42_RS18505 0.41 +0.3 4,005,229 - RR42_RS18505 0.42 +0.3 4,005,321 + RR42_RS18505 0.50 +1.1 4,005,322 - RR42_RS18505 0.50 +1.1 4,005,322 - RR42_RS18505 0.50 +0.2 4,005,322 - RR42_RS18505 0.50 +0.3 4,005,360 + RR42_RS18505 0.53 -1.0 4,005,361 - RR42_RS18505 0.53 +0.1 4,005,361 - RR42_RS18505 0.53 -0.8 4,005,361 - RR42_RS18505 0.53 +0.9 4,005,475 + RR42_RS18505 0.62 -1.1 4,005,580 - RR42_RS18505 0.71 +0.8 4,005,580 - RR42_RS18505 0.71 -1.5 4,005,580 - RR42_RS18505 0.71 +0.1 4,005,967 + +0.0 4,005,968 - +0.2 4,005,968 - -1.7 4,005,976 + -0.3 4,005,976 + +0.3 4,005,976 + -0.1 4,005,977 - +0.3 4,005,977 - +0.3 4,005,977 - +1.0 4,006,041 + +1.0 4,006,042 - -0.5 4,006,353 - RR42_RS18510 0.54 -0.4 4,006,417 + RR42_RS18510 0.69 -0.4 4,006,417 + RR42_RS18510 0.69 -0.5 4,006,417 + RR42_RS18510 0.69 -0.9 4,006,418 - RR42_RS18510 0.69 -0.5 4,006,418 - RR42_RS18510 0.69 +0.8 4,006,581 + +1.0 4,006,582 - +0.5 4,006,582 - +0.2 4,006,612 + -1.3 4,006,612 + -1.2 4,006,613 - +0.1 4,006,613 - -0.7 4,006,613 - -0.7 4,006,613 - +0.8 4,006,613 - -0.5 4,006,613 - -0.7 4,006,616 + +1.3 4,006,616 + -1.0 4,006,616 + +0.1 4,006,616 + +0.3 4,006,616 + +0.1 4,006,616 + -0.8 4,006,616 + -0.5 4,006,616 + -0.6 4,006,616 + -1.8 4,006,616 + +0.4 4,006,617 - -0.8 4,006,617 - -0.5 4,006,876 + RR42_RS18515 0.27 -0.2 4,006,876 + RR42_RS18515 0.27 -0.0 4,006,876 + RR42_RS18515 0.27 +0.3 4,006,876 + RR42_RS18515 0.27 -0.1 4,006,885 + RR42_RS18515 0.28 +1.3 4,006,886 - RR42_RS18515 0.28 -0.3 4,006,981 + RR42_RS18515 0.40 -1.0 4,006,982 - RR42_RS18515 0.40 +2.1 4,007,120 - RR42_RS18515 0.57 -0.2 4,007,297 - RR42_RS18515 0.79 -1.0 4,007,380 + RR42_RS18515 0.89 -2.3 4,007,380 + RR42_RS18515 0.89 -1.4 4,007,380 + RR42_RS18515 0.89 -0.9 4,007,380 + RR42_RS18515 0.89 -1.5 4,007,380 + RR42_RS18515 0.89 -0.3 4,007,380 + RR42_RS18515 0.89 +1.6 4,007,381 - RR42_RS18515 0.89 -0.1 4,007,381 - RR42_RS18515 0.89 -0.3 4,007,381 - RR42_RS18515 0.89 -2.0 4,007,381 - RR42_RS18515 0.89 -1.1 4,007,381 - RR42_RS18515 0.89 +0.4 4,007,381 - RR42_RS18515 0.89 +0.6 4,007,522 - +0.2 4,007,522 - -0.6 4,007,522 - -0.9
Or see this region's nucleotide sequence