Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18000 and RR42_RS18005 are separated by 4 nucleotides RR42_RS18005 and RR42_RS18010 are separated by 39 nucleotides RR42_RS18010 and RR42_RS18015 overlap by 4 nucleotides
RR42_RS18000: RR42_RS18000 - cell division protein ZapD, at 3,893,931 to 3,894,689
_RS18000
RR42_RS18005: RR42_RS18005 - pilus assembly protein, at 3,894,694 to 3,894,879
_RS18005
RR42_RS18010: RR42_RS18010 - 7,8-dihydro-8-oxoguanine-triphosphatase, at 3,894,919 to 3,895,359
_RS18010
RR42_RS18015: RR42_RS18015 - ATPase AAA, at 3,895,356 to 3,896,258
_RS18015
Position (kb)
3894
3895
3896 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3893.923 kb on + strand at 3893.923 kb on + strand at 3893.923 kb on + strand at 3893.924 kb on - strand at 3893.924 kb on - strand at 3893.940 kb on + strand at 3893.946 kb on + strand at 3893.947 kb on - strand at 3893.954 kb on + strand at 3893.955 kb on - strand at 3894.009 kb on + strand, within RR42_RS18000 at 3894.010 kb on - strand, within RR42_RS18000 at 3894.342 kb on + strand, within RR42_RS18000 at 3894.342 kb on + strand, within RR42_RS18000 at 3894.342 kb on + strand, within RR42_RS18000 at 3894.343 kb on - strand, within RR42_RS18000 at 3894.343 kb on - strand, within RR42_RS18000 at 3894.343 kb on - strand, within RR42_RS18000 at 3894.343 kb on - strand, within RR42_RS18000 at 3894.495 kb on + strand, within RR42_RS18000 at 3894.495 kb on + strand, within RR42_RS18000 at 3894.495 kb on + strand, within RR42_RS18000 at 3894.496 kb on - strand, within RR42_RS18000 at 3894.522 kb on + strand, within RR42_RS18000 at 3894.522 kb on + strand, within RR42_RS18000 at 3894.523 kb on - strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.591 kb on + strand, within RR42_RS18000 at 3894.592 kb on - strand, within RR42_RS18000 at 3894.592 kb on - strand, within RR42_RS18000 at 3894.592 kb on - strand, within RR42_RS18000 at 3894.592 kb on - strand, within RR42_RS18000 at 3894.592 kb on - strand, within RR42_RS18000 at 3894.704 kb on + strand at 3894.704 kb on + strand at 3894.704 kb on + strand at 3894.753 kb on + strand, within RR42_RS18005 at 3894.754 kb on - strand, within RR42_RS18005 at 3894.754 kb on - strand, within RR42_RS18005 at 3894.820 kb on + strand, within RR42_RS18005 at 3894.821 kb on - strand, within RR42_RS18005 at 3894.821 kb on - strand, within RR42_RS18005 at 3894.877 kb on + strand at 3894.877 kb on + strand at 3894.878 kb on - strand at 3894.878 kb on - strand at 3894.878 kb on - strand at 3894.878 kb on - strand at 3894.878 kb on - strand at 3894.878 kb on - strand at 3894.966 kb on + strand, within RR42_RS18010 at 3894.966 kb on + strand, within RR42_RS18010 at 3894.985 kb on + strand, within RR42_RS18010 at 3894.985 kb on + strand, within RR42_RS18010 at 3894.985 kb on + strand, within RR42_RS18010 at 3894.985 kb on + strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3894.986 kb on - strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.082 kb on + strand, within RR42_RS18010 at 3895.083 kb on - strand, within RR42_RS18010 at 3895.083 kb on - strand, within RR42_RS18010 at 3895.083 kb on - strand, within RR42_RS18010 at 3895.095 kb on - strand, within RR42_RS18010 at 3895.095 kb on - strand, within RR42_RS18010 at 3895.232 kb on + strand, within RR42_RS18010 at 3895.232 kb on + strand, within RR42_RS18010 at 3895.233 kb on - strand, within RR42_RS18010 at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.387 kb on + strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.388 kb on - strand at 3895.552 kb on + strand, within RR42_RS18015 at 3895.552 kb on + strand, within RR42_RS18015 at 3895.553 kb on - strand, within RR42_RS18015 at 3895.553 kb on - strand, within RR42_RS18015 at 3895.553 kb on - strand, within RR42_RS18015 at 3895.553 kb on - strand, within RR42_RS18015 at 3895.573 kb on + strand, within RR42_RS18015 at 3895.666 kb on + strand, within RR42_RS18015 at 3895.667 kb on - strand, within RR42_RS18015 at 3895.687 kb on + strand, within RR42_RS18015 at 3895.723 kb on + strand, within RR42_RS18015 at 3895.723 kb on + strand, within RR42_RS18015 at 3895.723 kb on + strand, within RR42_RS18015 at 3895.724 kb on - strand, within RR42_RS18015 at 3896.030 kb on + strand, within RR42_RS18015 at 3896.031 kb on - strand, within RR42_RS18015 at 3896.031 kb on - strand, within RR42_RS18015 at 3896.116 kb on + strand, within RR42_RS18015 at 3896.117 kb on - strand, within RR42_RS18015 at 3896.231 kb on + strand at 3896.231 kb on + strand at 3896.231 kb on + strand at 3896.231 kb on + strand at 3896.232 kb on - strand at 3896.232 kb on - strand at 3896.232 kb on - strand at 3896.232 kb on - strand at 3896.232 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,893,923 + +0.5 3,893,923 + +0.1 3,893,923 + -1.2 3,893,924 - -0.4 3,893,924 - -3.9 3,893,940 + +0.6 3,893,946 + +0.4 3,893,947 - -0.3 3,893,954 + +0.6 3,893,955 - -0.1 3,894,009 + RR42_RS18000 0.10 +0.3 3,894,010 - RR42_RS18000 0.10 +0.2 3,894,342 + RR42_RS18000 0.54 +0.1 3,894,342 + RR42_RS18000 0.54 +0.1 3,894,342 + RR42_RS18000 0.54 +0.5 3,894,343 - RR42_RS18000 0.54 +0.5 3,894,343 - RR42_RS18000 0.54 -0.4 3,894,343 - RR42_RS18000 0.54 +0.1 3,894,343 - RR42_RS18000 0.54 -0.7 3,894,495 + RR42_RS18000 0.74 +0.6 3,894,495 + RR42_RS18000 0.74 -0.1 3,894,495 + RR42_RS18000 0.74 -0.4 3,894,496 - RR42_RS18000 0.74 +0.3 3,894,522 + RR42_RS18000 0.78 +0.3 3,894,522 + RR42_RS18000 0.78 +1.7 3,894,523 - RR42_RS18000 0.78 +0.1 3,894,591 + RR42_RS18000 0.87 +0.0 3,894,591 + RR42_RS18000 0.87 -1.4 3,894,591 + RR42_RS18000 0.87 -0.2 3,894,591 + RR42_RS18000 0.87 -0.0 3,894,591 + RR42_RS18000 0.87 -0.1 3,894,591 + RR42_RS18000 0.87 +0.1 3,894,592 - RR42_RS18000 0.87 +0.6 3,894,592 - RR42_RS18000 0.87 +0.5 3,894,592 - RR42_RS18000 0.87 -0.5 3,894,592 - RR42_RS18000 0.87 -0.1 3,894,592 - RR42_RS18000 0.87 +0.8 3,894,704 + +1.6 3,894,704 + -0.5 3,894,704 + +0.0 3,894,753 + RR42_RS18005 0.32 -1.0 3,894,754 - RR42_RS18005 0.32 -0.3 3,894,754 - RR42_RS18005 0.32 -0.0 3,894,820 + RR42_RS18005 0.68 +0.1 3,894,821 - RR42_RS18005 0.68 -2.6 3,894,821 - RR42_RS18005 0.68 -0.4 3,894,877 + +0.3 3,894,877 + +0.9 3,894,878 - -0.1 3,894,878 - +0.6 3,894,878 - -0.0 3,894,878 - -0.5 3,894,878 - -0.4 3,894,878 - -0.3 3,894,966 + RR42_RS18010 0.11 -0.1 3,894,966 + RR42_RS18010 0.11 -0.8 3,894,985 + RR42_RS18010 0.15 -0.3 3,894,985 + RR42_RS18010 0.15 -0.4 3,894,985 + RR42_RS18010 0.15 +0.8 3,894,985 + RR42_RS18010 0.15 +0.5 3,894,986 - RR42_RS18010 0.15 -0.7 3,894,986 - RR42_RS18010 0.15 +0.3 3,894,986 - RR42_RS18010 0.15 +0.6 3,894,986 - RR42_RS18010 0.15 -0.2 3,894,986 - RR42_RS18010 0.15 -0.6 3,894,986 - RR42_RS18010 0.15 +0.1 3,894,986 - RR42_RS18010 0.15 -1.2 3,895,082 + RR42_RS18010 0.37 +0.3 3,895,082 + RR42_RS18010 0.37 -0.0 3,895,082 + RR42_RS18010 0.37 -0.2 3,895,082 + RR42_RS18010 0.37 +0.4 3,895,082 + RR42_RS18010 0.37 -1.6 3,895,082 + RR42_RS18010 0.37 -1.2 3,895,082 + RR42_RS18010 0.37 -0.2 3,895,082 + RR42_RS18010 0.37 -1.4 3,895,082 + RR42_RS18010 0.37 +0.7 3,895,082 + RR42_RS18010 0.37 +0.1 3,895,083 - RR42_RS18010 0.37 +0.1 3,895,083 - RR42_RS18010 0.37 -1.0 3,895,083 - RR42_RS18010 0.37 -0.4 3,895,095 - RR42_RS18010 0.40 -0.2 3,895,095 - RR42_RS18010 0.40 -1.5 3,895,232 + RR42_RS18010 0.71 -0.1 3,895,232 + RR42_RS18010 0.71 -0.5 3,895,233 - RR42_RS18010 0.71 +0.7 3,895,387 + -0.9 3,895,387 + -0.0 3,895,387 + -0.4 3,895,387 + +0.1 3,895,387 + -0.1 3,895,387 + -0.8 3,895,387 + -1.2 3,895,388 - -0.1 3,895,388 - -0.4 3,895,388 - +1.1 3,895,388 - +0.2 3,895,388 - +0.1 3,895,388 - +1.1 3,895,388 - +0.1 3,895,388 - +0.1 3,895,388 - -0.0 3,895,388 - +0.1 3,895,552 + RR42_RS18015 0.22 +0.7 3,895,552 + RR42_RS18015 0.22 +0.2 3,895,553 - RR42_RS18015 0.22 +0.3 3,895,553 - RR42_RS18015 0.22 +0.3 3,895,553 - RR42_RS18015 0.22 +0.2 3,895,553 - RR42_RS18015 0.22 -0.2 3,895,573 + RR42_RS18015 0.24 -1.3 3,895,666 + RR42_RS18015 0.34 -0.6 3,895,667 - RR42_RS18015 0.34 -0.4 3,895,687 + RR42_RS18015 0.37 -0.0 3,895,723 + RR42_RS18015 0.41 -0.5 3,895,723 + RR42_RS18015 0.41 +0.1 3,895,723 + RR42_RS18015 0.41 -0.3 3,895,724 - RR42_RS18015 0.41 +0.4 3,896,030 + RR42_RS18015 0.75 +0.0 3,896,031 - RR42_RS18015 0.75 -0.3 3,896,031 - RR42_RS18015 0.75 +0.6 3,896,116 + RR42_RS18015 0.84 +0.1 3,896,117 - RR42_RS18015 0.84 -1.1 3,896,231 + -0.3 3,896,231 + -0.3 3,896,231 + -0.2 3,896,231 + -0.3 3,896,232 - -0.2 3,896,232 - +0.8 3,896,232 - +0.7 3,896,232 - +0.1 3,896,232 - -0.2
Or see this region's nucleotide sequence