Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17465 and RR42_RS17470 overlap by 4 nucleotides RR42_RS17470 and RR42_RS17475 are separated by 87 nucleotides RR42_RS17475 and RR42_RS17480 are separated by 4 nucleotides RR42_RS17480 and RR42_RS17485 are separated by 126 nucleotides
RR42_RS17465: RR42_RS17465 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 3,781,159 to 3,782,259
_RS17465
RR42_RS17470: RR42_RS17470 - NUDIX hydrolase, at 3,782,256 to 3,782,768
_RS17470
RR42_RS17475: RR42_RS17475 - dihydrodipicolinate synthase, at 3,782,856 to 3,783,767
_RS17475
RR42_RS17480: RR42_RS17480 - hypothetical protein, at 3,783,772 to 3,784,170
_RS17480
RR42_RS17485: RR42_RS17485 - hypothetical protein, at 3,784,297 to 3,785,109
_RS17485
Position (kb)
3782
3783
3784 Strain fitness (log2 ratio)
-1
0
1
2 at 3782.297 kb on - strand at 3782.472 kb on - strand, within RR42_RS17470 at 3782.489 kb on - strand, within RR42_RS17470 at 3782.489 kb on - strand, within RR42_RS17470 at 3782.489 kb on - strand, within RR42_RS17470 at 3782.489 kb on - strand, within RR42_RS17470 at 3782.489 kb on - strand, within RR42_RS17470 at 3782.489 kb on - strand, within RR42_RS17470 at 3782.493 kb on - strand, within RR42_RS17470 at 3782.493 kb on - strand, within RR42_RS17470 at 3782.546 kb on - strand, within RR42_RS17470 at 3782.546 kb on - strand, within RR42_RS17470 at 3782.546 kb on - strand, within RR42_RS17470 at 3782.546 kb on - strand, within RR42_RS17470 at 3782.791 kb on - strand at 3782.791 kb on - strand at 3782.857 kb on + strand at 3782.857 kb on + strand at 3782.857 kb on + strand at 3782.857 kb on + strand at 3783.323 kb on + strand, within RR42_RS17475 at 3783.323 kb on + strand, within RR42_RS17475 at 3783.323 kb on + strand, within RR42_RS17475 at 3783.323 kb on + strand, within RR42_RS17475 at 3783.323 kb on + strand, within RR42_RS17475 at 3783.323 kb on + strand, within RR42_RS17475 at 3783.323 kb on + strand, within RR42_RS17475 at 3783.323 kb on + strand, within RR42_RS17475 at 3783.323 kb on + strand, within RR42_RS17475 at 3783.324 kb on - strand, within RR42_RS17475 at 3783.324 kb on - strand, within RR42_RS17475 at 3783.324 kb on - strand, within RR42_RS17475 at 3783.324 kb on - strand, within RR42_RS17475 at 3783.352 kb on + strand, within RR42_RS17475 at 3783.353 kb on - strand, within RR42_RS17475 at 3783.353 kb on - strand, within RR42_RS17475 at 3783.353 kb on - strand, within RR42_RS17475 at 3783.353 kb on - strand at 3783.353 kb on - strand, within RR42_RS17475 at 3783.430 kb on + strand, within RR42_RS17475 at 3783.430 kb on + strand, within RR42_RS17475 at 3783.433 kb on + strand, within RR42_RS17475 at 3783.434 kb on - strand, within RR42_RS17475 at 3783.658 kb on + strand, within RR42_RS17475 at 3783.658 kb on + strand, within RR42_RS17475 at 3783.658 kb on + strand, within RR42_RS17475 at 3783.659 kb on - strand, within RR42_RS17475 at 3783.659 kb on - strand, within RR42_RS17475 at 3783.659 kb on - strand, within RR42_RS17475 at 3783.659 kb on - strand, within RR42_RS17475 at 3783.659 kb on - strand, within RR42_RS17475 at 3783.659 kb on - strand, within RR42_RS17475 at 3783.659 kb on - strand, within RR42_RS17475 at 3783.659 kb on - strand, within RR42_RS17475 at 3783.929 kb on + strand, within RR42_RS17480 at 3783.930 kb on - strand, within RR42_RS17480 at 3784.221 kb on + strand at 3784.221 kb on + strand at 3784.221 kb on + strand at 3784.221 kb on + strand at 3784.222 kb on - strand at 3784.222 kb on - strand at 3784.222 kb on - strand at 3784.222 kb on - strand at 3784.222 kb on - strand at 3784.222 kb on - strand at 3784.249 kb on + strand at 3784.250 kb on - strand at 3784.342 kb on - strand at 3784.342 kb on - strand at 3784.343 kb on + strand at 3784.392 kb on + strand, within RR42_RS17485 at 3784.392 kb on + strand, within RR42_RS17485 at 3784.392 kb on + strand, within RR42_RS17485 at 3784.392 kb on + strand, within RR42_RS17485 at 3784.393 kb on - strand, within RR42_RS17485 at 3784.394 kb on + strand, within RR42_RS17485 at 3784.394 kb on + strand, within RR42_RS17485 at 3784.395 kb on - strand, within RR42_RS17485 at 3784.457 kb on + strand, within RR42_RS17485 at 3784.552 kb on + strand, within RR42_RS17485 at 3784.553 kb on - strand, within RR42_RS17485 at 3784.556 kb on + strand, within RR42_RS17485 at 3784.556 kb on + strand, within RR42_RS17485 at 3784.556 kb on + strand, within RR42_RS17485 at 3784.557 kb on - strand, within RR42_RS17485 at 3784.557 kb on - strand, within RR42_RS17485 at 3784.557 kb on - strand, within RR42_RS17485 at 3784.557 kb on - strand, within RR42_RS17485 at 3784.557 kb on - strand, within RR42_RS17485 at 3784.557 kb on - strand, within RR42_RS17485 at 3784.559 kb on + strand, within RR42_RS17485 at 3784.559 kb on + strand, within RR42_RS17485 at 3784.559 kb on + strand, within RR42_RS17485 at 3784.560 kb on - strand, within RR42_RS17485 at 3784.560 kb on - strand, within RR42_RS17485 at 3784.560 kb on - strand, within RR42_RS17485 at 3784.560 kb on - strand, within RR42_RS17485 at 3784.560 kb on - strand, within RR42_RS17485 at 3784.632 kb on - strand, within RR42_RS17485 at 3784.662 kb on - strand, within RR42_RS17485 at 3784.673 kb on + strand, within RR42_RS17485 at 3784.674 kb on - strand, within RR42_RS17485 at 3784.700 kb on + strand, within RR42_RS17485 at 3784.700 kb on + strand, within RR42_RS17485 at 3784.700 kb on + strand, within RR42_RS17485 at 3784.700 kb on + strand, within RR42_RS17485 at 3784.700 kb on + strand, within RR42_RS17485 at 3784.700 kb on + strand, within RR42_RS17485 at 3784.700 kb on + strand, within RR42_RS17485 at 3784.701 kb on - strand, within RR42_RS17485 at 3784.701 kb on - strand, within RR42_RS17485 at 3784.701 kb on - strand, within RR42_RS17485 at 3784.701 kb on - strand, within RR42_RS17485 at 3784.701 kb on - strand, within RR42_RS17485 at 3784.703 kb on + strand, within RR42_RS17485 at 3784.703 kb on + strand, within RR42_RS17485 at 3784.703 kb on + strand, within RR42_RS17485 at 3784.703 kb on + strand, within RR42_RS17485 at 3784.704 kb on - strand, within RR42_RS17485 at 3784.704 kb on - strand, within RR42_RS17485 at 3784.704 kb on - strand, within RR42_RS17485 at 3784.704 kb on - strand, within RR42_RS17485 at 3784.724 kb on + strand, within RR42_RS17485 at 3784.725 kb on - strand, within RR42_RS17485
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,782,297 - +0.3 3,782,472 - RR42_RS17470 0.42 +0.1 3,782,489 - RR42_RS17470 0.45 -0.2 3,782,489 - RR42_RS17470 0.45 -0.7 3,782,489 - RR42_RS17470 0.45 +0.4 3,782,489 - RR42_RS17470 0.45 -1.2 3,782,489 - RR42_RS17470 0.45 +0.1 3,782,489 - RR42_RS17470 0.45 +1.1 3,782,493 - RR42_RS17470 0.46 +0.8 3,782,493 - RR42_RS17470 0.46 +1.2 3,782,546 - RR42_RS17470 0.57 +0.6 3,782,546 - RR42_RS17470 0.57 -0.0 3,782,546 - RR42_RS17470 0.57 +0.8 3,782,546 - RR42_RS17470 0.57 -0.5 3,782,791 - +2.3 3,782,791 - +0.0 3,782,857 + +0.5 3,782,857 + -0.0 3,782,857 + +1.3 3,782,857 + +0.2 3,783,323 + RR42_RS17475 0.51 -0.9 3,783,323 + RR42_RS17475 0.51 +0.3 3,783,323 + RR42_RS17475 0.51 +0.2 3,783,323 + RR42_RS17475 0.51 -0.6 3,783,323 + RR42_RS17475 0.51 -0.3 3,783,323 + RR42_RS17475 0.51 -1.0 3,783,323 + RR42_RS17475 0.51 +1.1 3,783,323 + RR42_RS17475 0.51 -0.1 3,783,323 + RR42_RS17475 0.51 -0.1 3,783,324 - RR42_RS17475 0.51 -0.2 3,783,324 - RR42_RS17475 0.51 -0.4 3,783,324 - RR42_RS17475 0.51 -0.2 3,783,324 - RR42_RS17475 0.51 -1.4 3,783,352 + RR42_RS17475 0.54 +0.3 3,783,353 - RR42_RS17475 0.54 +0.2 3,783,353 - RR42_RS17475 0.54 +0.2 3,783,353 - RR42_RS17475 0.54 +0.9 3,783,353 - +0.3 3,783,353 - RR42_RS17475 0.54 -0.8 3,783,430 + RR42_RS17475 0.63 -0.8 3,783,430 + RR42_RS17475 0.63 -1.0 3,783,433 + RR42_RS17475 0.63 +0.0 3,783,434 - RR42_RS17475 0.63 -0.3 3,783,658 + RR42_RS17475 0.88 -0.7 3,783,658 + RR42_RS17475 0.88 +0.5 3,783,658 + RR42_RS17475 0.88 +0.0 3,783,659 - RR42_RS17475 0.88 -0.1 3,783,659 - RR42_RS17475 0.88 -0.4 3,783,659 - RR42_RS17475 0.88 -1.0 3,783,659 - RR42_RS17475 0.88 +0.3 3,783,659 - RR42_RS17475 0.88 -1.2 3,783,659 - RR42_RS17475 0.88 -0.3 3,783,659 - RR42_RS17475 0.88 -0.7 3,783,659 - RR42_RS17475 0.88 +0.3 3,783,929 + RR42_RS17480 0.39 +0.7 3,783,930 - RR42_RS17480 0.40 -0.3 3,784,221 + +0.3 3,784,221 + +0.2 3,784,221 + -0.2 3,784,221 + +0.3 3,784,222 - +0.2 3,784,222 - -0.3 3,784,222 - +0.7 3,784,222 - -0.4 3,784,222 - -0.6 3,784,222 - +0.3 3,784,249 + +1.1 3,784,250 - -0.0 3,784,342 - +0.3 3,784,342 - -0.2 3,784,343 + -0.3 3,784,392 + RR42_RS17485 0.12 -0.2 3,784,392 + RR42_RS17485 0.12 -0.1 3,784,392 + RR42_RS17485 0.12 +1.4 3,784,392 + RR42_RS17485 0.12 +0.5 3,784,393 - RR42_RS17485 0.12 -0.5 3,784,394 + RR42_RS17485 0.12 -0.2 3,784,394 + RR42_RS17485 0.12 -0.1 3,784,395 - RR42_RS17485 0.12 -0.2 3,784,457 + RR42_RS17485 0.20 +0.2 3,784,552 + RR42_RS17485 0.31 +0.5 3,784,553 - RR42_RS17485 0.31 +0.0 3,784,556 + RR42_RS17485 0.32 -0.2 3,784,556 + RR42_RS17485 0.32 +0.9 3,784,556 + RR42_RS17485 0.32 -0.7 3,784,557 - RR42_RS17485 0.32 -0.3 3,784,557 - RR42_RS17485 0.32 +0.1 3,784,557 - RR42_RS17485 0.32 +1.2 3,784,557 - RR42_RS17485 0.32 +0.0 3,784,557 - RR42_RS17485 0.32 -0.0 3,784,557 - RR42_RS17485 0.32 +0.3 3,784,559 + RR42_RS17485 0.32 +0.6 3,784,559 + RR42_RS17485 0.32 +0.1 3,784,559 + RR42_RS17485 0.32 +0.2 3,784,560 - RR42_RS17485 0.32 +0.3 3,784,560 - RR42_RS17485 0.32 +0.0 3,784,560 - RR42_RS17485 0.32 +0.1 3,784,560 - RR42_RS17485 0.32 -1.5 3,784,560 - RR42_RS17485 0.32 +0.3 3,784,632 - RR42_RS17485 0.41 -0.2 3,784,662 - RR42_RS17485 0.45 +0.6 3,784,673 + RR42_RS17485 0.46 +0.6 3,784,674 - RR42_RS17485 0.46 -1.2 3,784,700 + RR42_RS17485 0.50 +0.4 3,784,700 + RR42_RS17485 0.50 -0.2 3,784,700 + RR42_RS17485 0.50 +0.1 3,784,700 + RR42_RS17485 0.50 -0.1 3,784,700 + RR42_RS17485 0.50 -0.2 3,784,700 + RR42_RS17485 0.50 -0.4 3,784,700 + RR42_RS17485 0.50 -0.1 3,784,701 - RR42_RS17485 0.50 -1.1 3,784,701 - RR42_RS17485 0.50 -0.5 3,784,701 - RR42_RS17485 0.50 +0.1 3,784,701 - RR42_RS17485 0.50 -0.7 3,784,701 - RR42_RS17485 0.50 +1.0 3,784,703 + RR42_RS17485 0.50 -0.1 3,784,703 + RR42_RS17485 0.50 +0.9 3,784,703 + RR42_RS17485 0.50 +0.0 3,784,703 + RR42_RS17485 0.50 +0.0 3,784,704 - RR42_RS17485 0.50 -0.2 3,784,704 - RR42_RS17485 0.50 +0.7 3,784,704 - RR42_RS17485 0.50 -0.5 3,784,704 - RR42_RS17485 0.50 +0.8 3,784,724 + RR42_RS17485 0.53 -0.2 3,784,725 - RR42_RS17485 0.53 -0.4
Or see this region's nucleotide sequence