Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16060 and RR42_RS16065 are separated by 224 nucleotides RR42_RS16065 and RR42_RS16070 overlap by 50 nucleotides RR42_RS16070 and RR42_RS16075 are separated by 199 nucleotides
RR42_RS16060: RR42_RS16060 - membrane protein, at 3,479,144 to 3,480,319
_RS16060
RR42_RS16065: RR42_RS16065 - GNAT family acetyltransferase, at 3,480,544 to 3,481,125
_RS16065
RR42_RS16070: RR42_RS16070 - cobyrinic acid a,c-diamide synthase, at 3,481,076 to 3,481,726
_RS16070
RR42_RS16075: RR42_RS16075 - cobyric acid synthase, at 3,481,926 to 3,483,386
_RS16075
Position (kb)
3480
3481
3482 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3479.719 kb on + strand, within RR42_RS16060 at 3479.719 kb on + strand, within RR42_RS16060 at 3479.720 kb on - strand, within RR42_RS16060 at 3479.880 kb on + strand, within RR42_RS16060 at 3480.028 kb on - strand, within RR42_RS16060 at 3480.038 kb on - strand, within RR42_RS16060 at 3480.087 kb on + strand, within RR42_RS16060 at 3480.087 kb on + strand, within RR42_RS16060 at 3480.152 kb on + strand, within RR42_RS16060 at 3480.153 kb on - strand, within RR42_RS16060 at 3480.153 kb on - strand, within RR42_RS16060 at 3480.274 kb on - strand at 3480.431 kb on + strand at 3480.431 kb on + strand at 3480.432 kb on - strand at 3480.631 kb on + strand, within RR42_RS16065 at 3480.631 kb on + strand, within RR42_RS16065 at 3480.631 kb on + strand, within RR42_RS16065 at 3480.632 kb on - strand, within RR42_RS16065 at 3480.632 kb on - strand, within RR42_RS16065 at 3480.805 kb on + strand, within RR42_RS16065 at 3480.806 kb on - strand, within RR42_RS16065 at 3480.806 kb on - strand, within RR42_RS16065 at 3480.814 kb on + strand, within RR42_RS16065 at 3480.814 kb on + strand, within RR42_RS16065 at 3480.815 kb on - strand, within RR42_RS16065 at 3480.815 kb on - strand, within RR42_RS16065 at 3480.931 kb on + strand, within RR42_RS16065 at 3480.931 kb on + strand, within RR42_RS16065 at 3480.932 kb on - strand, within RR42_RS16065 at 3480.932 kb on - strand, within RR42_RS16065 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.209 kb on + strand, within RR42_RS16070 at 3481.210 kb on - strand, within RR42_RS16070 at 3481.210 kb on - strand, within RR42_RS16070 at 3481.483 kb on + strand, within RR42_RS16070 at 3481.484 kb on - strand, within RR42_RS16070 at 3481.484 kb on - strand, within RR42_RS16070 at 3481.484 kb on - strand, within RR42_RS16070 at 3481.501 kb on + strand, within RR42_RS16070 at 3481.654 kb on - strand, within RR42_RS16070 at 3481.654 kb on - strand, within RR42_RS16070 at 3481.689 kb on - strand at 3481.719 kb on - strand at 3481.847 kb on + strand at 3481.848 kb on - strand at 3481.928 kb on - strand at 3481.928 kb on - strand at 3482.107 kb on + strand, within RR42_RS16075 at 3482.107 kb on + strand, within RR42_RS16075 at 3482.108 kb on - strand, within RR42_RS16075 at 3482.108 kb on - strand, within RR42_RS16075 at 3482.108 kb on - strand, within RR42_RS16075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,479,719 + RR42_RS16060 0.49 -0.7 3,479,719 + RR42_RS16060 0.49 -0.9 3,479,720 - RR42_RS16060 0.49 -3.1 3,479,880 + RR42_RS16060 0.63 -0.8 3,480,028 - RR42_RS16060 0.75 -0.2 3,480,038 - RR42_RS16060 0.76 +0.5 3,480,087 + RR42_RS16060 0.80 -1.6 3,480,087 + RR42_RS16060 0.80 -0.5 3,480,152 + RR42_RS16060 0.86 +0.7 3,480,153 - RR42_RS16060 0.86 +1.3 3,480,153 - RR42_RS16060 0.86 -3.1 3,480,274 - -0.5 3,480,431 + +0.4 3,480,431 + +0.9 3,480,432 - +0.7 3,480,631 + RR42_RS16065 0.15 +0.9 3,480,631 + RR42_RS16065 0.15 -0.3 3,480,631 + RR42_RS16065 0.15 +0.3 3,480,632 - RR42_RS16065 0.15 +0.4 3,480,632 - RR42_RS16065 0.15 -0.1 3,480,805 + RR42_RS16065 0.45 +0.8 3,480,806 - RR42_RS16065 0.45 -0.3 3,480,806 - RR42_RS16065 0.45 -0.1 3,480,814 + RR42_RS16065 0.46 -0.7 3,480,814 + RR42_RS16065 0.46 +0.6 3,480,815 - RR42_RS16065 0.47 -0.4 3,480,815 - RR42_RS16065 0.47 +0.8 3,480,931 + RR42_RS16065 0.66 -1.3 3,480,931 + RR42_RS16065 0.66 -0.3 3,480,932 - RR42_RS16065 0.67 -0.0 3,480,932 - RR42_RS16065 0.67 +0.4 3,481,209 + RR42_RS16070 0.20 -0.4 3,481,209 + RR42_RS16070 0.20 -1.1 3,481,209 + RR42_RS16070 0.20 -1.6 3,481,209 + RR42_RS16070 0.20 +1.8 3,481,209 + RR42_RS16070 0.20 -0.3 3,481,210 - RR42_RS16070 0.21 +0.5 3,481,210 - RR42_RS16070 0.21 -0.4 3,481,483 + RR42_RS16070 0.63 -2.7 3,481,484 - RR42_RS16070 0.63 -0.6 3,481,484 - RR42_RS16070 0.63 -0.5 3,481,484 - RR42_RS16070 0.63 -0.8 3,481,501 + RR42_RS16070 0.65 -0.3 3,481,654 - RR42_RS16070 0.89 +0.0 3,481,654 - RR42_RS16070 0.89 +0.2 3,481,689 - -0.6 3,481,719 - +2.6 3,481,847 + -0.6 3,481,848 - +0.2 3,481,928 - +0.5 3,481,928 - +1.3 3,482,107 + RR42_RS16075 0.12 -2.8 3,482,107 + RR42_RS16075 0.12 +0.2 3,482,108 - RR42_RS16075 0.12 -1.1 3,482,108 - RR42_RS16075 0.12 -0.3 3,482,108 - RR42_RS16075 0.12 +1.3
Or see this region's nucleotide sequence