Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15100 and RR42_RS15105 are separated by 89 nucleotides RR42_RS15105 and RR42_RS15110 are separated by 112 nucleotides RR42_RS15110 and RR42_RS15115 are separated by 58 nucleotides
RR42_RS15100: RR42_RS15100 - UTP--glucose-1-phosphate uridylyltransferase, at 3,269,255 to 3,270,133
_RS15100
RR42_RS15105: RR42_RS15105 - transcriptional regulator, at 3,270,223 to 3,270,450
_RS15105
RR42_RS15110: RR42_RS15110 - NADH pyrophosphatase, at 3,270,563 to 3,271,150
_RS15110
RR42_RS15115: RR42_RS15115 - threonine aldolase, at 3,271,209 to 3,272,240
_RS15115
Position (kb)
3270
3271
3272 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3270.485 kb on - strand at 3270.486 kb on - strand at 3270.506 kb on + strand at 3270.506 kb on + strand at 3270.507 kb on - strand at 3270.507 kb on - strand at 3270.565 kb on - strand at 3270.565 kb on - strand at 3270.565 kb on - strand at 3270.573 kb on + strand at 3270.578 kb on + strand at 3270.579 kb on - strand at 3270.751 kb on - strand, within RR42_RS15110 at 3270.751 kb on - strand, within RR42_RS15110 at 3270.751 kb on - strand, within RR42_RS15110 at 3270.801 kb on + strand, within RR42_RS15110 at 3270.801 kb on + strand, within RR42_RS15110 at 3270.802 kb on - strand, within RR42_RS15110 at 3270.802 kb on - strand, within RR42_RS15110 at 3270.838 kb on - strand, within RR42_RS15110 at 3271.002 kb on + strand, within RR42_RS15110 at 3271.037 kb on - strand, within RR42_RS15110 at 3271.134 kb on + strand at 3271.179 kb on + strand at 3271.179 kb on + strand at 3271.179 kb on + strand at 3271.179 kb on + strand at 3271.179 kb on + strand at 3271.179 kb on + strand at 3271.179 kb on + strand at 3271.179 kb on + strand at 3271.180 kb on - strand at 3271.180 kb on - strand at 3271.180 kb on - strand at 3271.180 kb on - strand at 3271.192 kb on + strand at 3271.193 kb on - strand at 3271.193 kb on - strand at 3271.195 kb on - strand at 3271.233 kb on + strand at 3271.313 kb on + strand, within RR42_RS15115 at 3271.313 kb on + strand, within RR42_RS15115 at 3271.313 kb on + strand, within RR42_RS15115 at 3271.313 kb on + strand, within RR42_RS15115 at 3271.313 kb on + strand, within RR42_RS15115 at 3271.313 kb on + strand, within RR42_RS15115 at 3271.313 kb on + strand, within RR42_RS15115 at 3271.314 kb on - strand, within RR42_RS15115 at 3271.315 kb on + strand, within RR42_RS15115 at 3271.315 kb on + strand, within RR42_RS15115 at 3271.315 kb on + strand, within RR42_RS15115 at 3271.316 kb on - strand, within RR42_RS15115 at 3271.316 kb on - strand, within RR42_RS15115 at 3271.520 kb on + strand, within RR42_RS15115 at 3271.565 kb on - strand, within RR42_RS15115 at 3271.795 kb on + strand, within RR42_RS15115 at 3271.982 kb on + strand, within RR42_RS15115 at 3271.983 kb on - strand, within RR42_RS15115 at 3271.983 kb on - strand, within RR42_RS15115 at 3272.015 kb on - strand, within RR42_RS15115 at 3272.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,270,485 - +0.5 3,270,486 - -0.1 3,270,506 + +1.0 3,270,506 + +0.4 3,270,507 - -2.0 3,270,507 - +0.6 3,270,565 - +0.3 3,270,565 - +0.6 3,270,565 - -0.4 3,270,573 + -0.3 3,270,578 + +1.3 3,270,579 - -0.7 3,270,751 - RR42_RS15110 0.32 -2.5 3,270,751 - RR42_RS15110 0.32 +0.3 3,270,751 - RR42_RS15110 0.32 -2.8 3,270,801 + RR42_RS15110 0.40 +0.5 3,270,801 + RR42_RS15110 0.40 -1.4 3,270,802 - RR42_RS15110 0.41 -0.6 3,270,802 - RR42_RS15110 0.41 -2.0 3,270,838 - RR42_RS15110 0.47 -0.5 3,271,002 + RR42_RS15110 0.75 -0.7 3,271,037 - RR42_RS15110 0.81 -1.0 3,271,134 + -0.1 3,271,179 + -0.8 3,271,179 + -0.5 3,271,179 + -0.6 3,271,179 + -1.1 3,271,179 + +0.9 3,271,179 + +0.1 3,271,179 + +1.5 3,271,179 + -0.3 3,271,180 - +0.9 3,271,180 - +0.5 3,271,180 - +0.4 3,271,180 - -0.2 3,271,192 + -0.4 3,271,193 - +1.5 3,271,193 - -0.6 3,271,195 - +0.3 3,271,233 + +1.0 3,271,313 + RR42_RS15115 0.10 +0.6 3,271,313 + RR42_RS15115 0.10 +0.9 3,271,313 + RR42_RS15115 0.10 +1.1 3,271,313 + RR42_RS15115 0.10 -2.0 3,271,313 + RR42_RS15115 0.10 +0.3 3,271,313 + RR42_RS15115 0.10 +0.1 3,271,313 + RR42_RS15115 0.10 -0.3 3,271,314 - RR42_RS15115 0.10 +0.1 3,271,315 + RR42_RS15115 0.10 -0.0 3,271,315 + RR42_RS15115 0.10 +0.3 3,271,315 + RR42_RS15115 0.10 -0.5 3,271,316 - RR42_RS15115 0.10 -0.4 3,271,316 - RR42_RS15115 0.10 -0.6 3,271,520 + RR42_RS15115 0.30 -0.1 3,271,565 - RR42_RS15115 0.34 -0.1 3,271,795 + RR42_RS15115 0.57 -0.4 3,271,982 + RR42_RS15115 0.75 -0.3 3,271,983 - RR42_RS15115 0.75 +0.3 3,271,983 - RR42_RS15115 0.75 -1.1 3,272,015 - RR42_RS15115 0.78 -1.1 3,272,147 - -0.5
Or see this region's nucleotide sequence