Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14610 and RR42_RS14615 are separated by 93 nucleotides RR42_RS14615 and RR42_RS14620 overlap by 7 nucleotides RR42_RS14620 and RR42_RS14625 are separated by 5 nucleotides RR42_RS14625 and RR42_RS14630 are separated by 14 nucleotides
RR42_RS14610: RR42_RS14610 - lactate permease, at 3,142,810 to 3,144,486
_RS14610
RR42_RS14615: RR42_RS14615 - pirin, at 3,144,580 to 3,145,005
_RS14615
RR42_RS14620: RR42_RS14620 - hypothetical protein, at 3,144,999 to 3,145,376
_RS14620
RR42_RS14625: RR42_RS14625 - formimidoylglutamase, at 3,145,382 to 3,146,320
_RS14625
RR42_RS14630: RR42_RS14630 - MFS transporter, at 3,146,335 to 3,147,642
_RS14630
Position (kb)
3144
3145
3146 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3144.014 kb on - strand, within RR42_RS14610 at 3144.040 kb on + strand, within RR42_RS14610 at 3144.041 kb on - strand, within RR42_RS14610 at 3144.041 kb on - strand, within RR42_RS14610 at 3144.361 kb on + strand at 3144.388 kb on + strand at 3144.388 kb on + strand at 3144.388 kb on + strand at 3144.389 kb on - strand at 3144.389 kb on - strand at 3144.389 kb on - strand at 3144.451 kb on + strand at 3144.451 kb on + strand at 3144.451 kb on + strand at 3144.451 kb on + strand at 3144.451 kb on + strand at 3144.451 kb on + strand at 3144.451 kb on + strand at 3144.451 kb on + strand at 3144.451 kb on + strand at 3144.451 kb on + strand at 3144.452 kb on - strand at 3144.452 kb on - strand at 3144.452 kb on - strand at 3144.452 kb on - strand at 3144.452 kb on - strand at 3144.452 kb on - strand at 3144.521 kb on + strand at 3144.522 kb on - strand at 3144.637 kb on - strand, within RR42_RS14615 at 3144.737 kb on - strand, within RR42_RS14615 at 3144.737 kb on - strand, within RR42_RS14615 at 3144.770 kb on + strand, within RR42_RS14615 at 3144.771 kb on - strand, within RR42_RS14615 at 3144.771 kb on - strand, within RR42_RS14615 at 3144.860 kb on + strand, within RR42_RS14615 at 3144.865 kb on + strand, within RR42_RS14615 at 3144.865 kb on + strand, within RR42_RS14615 at 3144.865 kb on + strand, within RR42_RS14615 at 3144.866 kb on - strand, within RR42_RS14615 at 3144.866 kb on - strand, within RR42_RS14615 at 3144.866 kb on - strand, within RR42_RS14615 at 3144.866 kb on - strand, within RR42_RS14615 at 3144.891 kb on + strand, within RR42_RS14615 at 3145.271 kb on + strand, within RR42_RS14620 at 3145.271 kb on + strand, within RR42_RS14620 at 3145.271 kb on + strand, within RR42_RS14620 at 3145.277 kb on + strand, within RR42_RS14620 at 3145.277 kb on + strand, within RR42_RS14620 at 3145.278 kb on - strand, within RR42_RS14620 at 3145.278 kb on - strand, within RR42_RS14620 at 3145.398 kb on + strand at 3145.443 kb on + strand at 3145.443 kb on + strand at 3145.443 kb on + strand at 3145.443 kb on + strand at 3145.444 kb on - strand at 3145.539 kb on + strand, within RR42_RS14625 at 3145.605 kb on + strand, within RR42_RS14625 at 3146.156 kb on + strand, within RR42_RS14625 at 3146.157 kb on - strand, within RR42_RS14625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,144,014 - RR42_RS14610 0.72 +0.0 3,144,040 + RR42_RS14610 0.73 -0.0 3,144,041 - RR42_RS14610 0.73 -0.1 3,144,041 - RR42_RS14610 0.73 +0.6 3,144,361 + +0.1 3,144,388 + -0.9 3,144,388 + -0.1 3,144,388 + +1.3 3,144,389 - +0.4 3,144,389 - -0.1 3,144,389 - -0.1 3,144,451 + -0.5 3,144,451 + +0.2 3,144,451 + +0.5 3,144,451 + +0.1 3,144,451 + +2.2 3,144,451 + +0.6 3,144,451 + -0.4 3,144,451 + -3.1 3,144,451 + +1.8 3,144,451 + -0.2 3,144,452 - -1.5 3,144,452 - +0.1 3,144,452 - -0.2 3,144,452 - -1.1 3,144,452 - +0.9 3,144,452 - +0.4 3,144,521 + -0.1 3,144,522 - -0.7 3,144,637 - RR42_RS14615 0.13 +0.6 3,144,737 - RR42_RS14615 0.37 -0.0 3,144,737 - RR42_RS14615 0.37 -0.3 3,144,770 + RR42_RS14615 0.45 -0.0 3,144,771 - RR42_RS14615 0.45 +0.5 3,144,771 - RR42_RS14615 0.45 +0.6 3,144,860 + RR42_RS14615 0.66 -1.6 3,144,865 + RR42_RS14615 0.67 -0.9 3,144,865 + RR42_RS14615 0.67 -0.3 3,144,865 + RR42_RS14615 0.67 -2.0 3,144,866 - RR42_RS14615 0.67 -0.7 3,144,866 - RR42_RS14615 0.67 -1.7 3,144,866 - RR42_RS14615 0.67 -0.0 3,144,866 - RR42_RS14615 0.67 +0.2 3,144,891 + RR42_RS14615 0.73 -1.3 3,145,271 + RR42_RS14620 0.72 +1.1 3,145,271 + RR42_RS14620 0.72 -0.7 3,145,271 + RR42_RS14620 0.72 -1.9 3,145,277 + RR42_RS14620 0.74 +0.3 3,145,277 + RR42_RS14620 0.74 -0.8 3,145,278 - RR42_RS14620 0.74 +0.6 3,145,278 - RR42_RS14620 0.74 -0.6 3,145,398 + -0.1 3,145,443 + +0.1 3,145,443 + +0.2 3,145,443 + -0.2 3,145,443 + +0.8 3,145,444 - -0.2 3,145,539 + RR42_RS14625 0.17 -2.8 3,145,605 + RR42_RS14625 0.24 -0.8 3,146,156 + RR42_RS14625 0.82 -1.2 3,146,157 - RR42_RS14625 0.83 +0.5
Or see this region's nucleotide sequence