Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14535 and RR42_RS14540 are separated by 121 nucleotides RR42_RS14540 and RR42_RS14545 are separated by 358 nucleotides
RR42_RS14535: RR42_RS14535 - hypothetical protein, at 3,128,406 to 3,129,551
_RS14535
RR42_RS14540: RR42_RS14540 - DNA glycosylase, at 3,129,673 to 3,130,062
_RS14540
RR42_RS14545: RR42_RS14545 - hypothetical protein, at 3,130,421 to 3,130,885
_RS14545
Position (kb) 3129
3130
3131 Strain fitness (log2 ratio) -1
0
1
2 at 3128.687 kb on + strand, within RR42_RS14535 at 3128.697 kb on + strand, within RR42_RS14535 at 3128.753 kb on + strand, within RR42_RS14535 at 3128.757 kb on + strand, within RR42_RS14535 at 3128.757 kb on + strand, within RR42_RS14535 at 3128.758 kb on - strand, within RR42_RS14535 at 3128.758 kb on - strand, within RR42_RS14535 at 3128.812 kb on - strand, within RR42_RS14535 at 3128.823 kb on + strand, within RR42_RS14535 at 3128.824 kb on - strand, within RR42_RS14535 at 3128.826 kb on - strand, within RR42_RS14535 at 3129.080 kb on + strand, within RR42_RS14535 at 3129.231 kb on - strand, within RR42_RS14535 at 3129.263 kb on - strand, within RR42_RS14535 at 3129.396 kb on + strand, within RR42_RS14535 at 3129.549 kb on + strand at 3129.550 kb on - strand at 3129.550 kb on - strand at 3129.756 kb on + strand, within RR42_RS14540 at 3129.969 kb on + strand, within RR42_RS14540 at 3130.005 kb on - strand, within RR42_RS14540 at 3130.182 kb on + strand at 3130.183 kb on - strand at 3130.303 kb on - strand at 3130.320 kb on + strand at 3130.321 kb on - strand at 3130.390 kb on + strand at 3130.390 kb on + strand at 3130.393 kb on - strand at 3130.515 kb on - strand, within RR42_RS14545 at 3130.515 kb on - strand, within RR42_RS14545 at 3130.515 kb on - strand, within RR42_RS14545 at 3130.516 kb on + strand, within RR42_RS14545 at 3130.517 kb on - strand, within RR42_RS14545 at 3130.532 kb on - strand, within RR42_RS14545 at 3130.569 kb on + strand, within RR42_RS14545 at 3130.694 kb on + strand, within RR42_RS14545 at 3130.695 kb on - strand, within RR42_RS14545 at 3130.703 kb on + strand, within RR42_RS14545 at 3130.704 kb on - strand, within RR42_RS14545 at 3130.732 kb on + strand, within RR42_RS14545 at 3130.732 kb on + strand, within RR42_RS14545 at 3130.777 kb on + strand, within RR42_RS14545 at 3130.778 kb on - strand, within RR42_RS14545 at 3130.778 kb on - strand, within RR42_RS14545 at 3130.816 kb on - strand, within RR42_RS14545 at 3130.820 kb on + strand, within RR42_RS14545 at 3130.820 kb on + strand, within RR42_RS14545 at 3130.821 kb on - strand, within RR42_RS14545 at 3130.821 kb on - strand, within RR42_RS14545 at 3130.821 kb on - strand, within RR42_RS14545
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,128,687 + RR42_RS14535 0.25 +0.2 3,128,697 + RR42_RS14535 0.25 +0.5 3,128,753 + RR42_RS14535 0.30 +0.8 3,128,757 + RR42_RS14535 0.31 +0.1 3,128,757 + RR42_RS14535 0.31 -1.2 3,128,758 - RR42_RS14535 0.31 +0.5 3,128,758 - RR42_RS14535 0.31 +0.6 3,128,812 - RR42_RS14535 0.35 -0.0 3,128,823 + RR42_RS14535 0.36 +0.0 3,128,824 - RR42_RS14535 0.36 -1.0 3,128,826 - RR42_RS14535 0.37 -0.1 3,129,080 + RR42_RS14535 0.59 +0.1 3,129,231 - RR42_RS14535 0.72 -1.5 3,129,263 - RR42_RS14535 0.75 +0.2 3,129,396 + RR42_RS14535 0.86 -0.5 3,129,549 + -0.3 3,129,550 - -1.1 3,129,550 - -1.0 3,129,756 + RR42_RS14540 0.21 -0.4 3,129,969 + RR42_RS14540 0.76 +0.1 3,130,005 - RR42_RS14540 0.85 -0.3 3,130,182 + +0.4 3,130,183 - -0.3 3,130,303 - +0.4 3,130,320 + +0.6 3,130,321 - -0.7 3,130,390 + -0.5 3,130,390 + -1.7 3,130,393 - +0.1 3,130,515 - RR42_RS14545 0.20 +2.0 3,130,515 - RR42_RS14545 0.20 +0.8 3,130,515 - RR42_RS14545 0.20 +0.5 3,130,516 + RR42_RS14545 0.20 +0.3 3,130,517 - RR42_RS14545 0.21 +0.0 3,130,532 - RR42_RS14545 0.24 -0.6 3,130,569 + RR42_RS14545 0.32 +0.3 3,130,694 + RR42_RS14545 0.59 +1.7 3,130,695 - RR42_RS14545 0.59 +0.1 3,130,703 + RR42_RS14545 0.61 +0.1 3,130,704 - RR42_RS14545 0.61 -0.3 3,130,732 + RR42_RS14545 0.67 +0.1 3,130,732 + RR42_RS14545 0.67 -0.3 3,130,777 + RR42_RS14545 0.77 -0.0 3,130,778 - RR42_RS14545 0.77 -0.3 3,130,778 - RR42_RS14545 0.77 -0.9 3,130,816 - RR42_RS14545 0.85 +0.7 3,130,820 + RR42_RS14545 0.86 -0.1 3,130,820 + RR42_RS14545 0.86 +0.0 3,130,821 - RR42_RS14545 0.86 -0.3 3,130,821 - RR42_RS14545 0.86 -0.1 3,130,821 - RR42_RS14545 0.86 +0.5
Or see this region's nucleotide sequence