Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14070 and RR42_RS14075 are separated by 8 nucleotides RR42_RS14075 and RR42_RS14080 are separated by 134 nucleotides RR42_RS14080 and RR42_RS14085 are separated by 129 nucleotides RR42_RS14085 and RR42_RS14090 are separated by 64 nucleotides
RR42_RS14070: RR42_RS14070 - sugar dehydratase, at 3,028,516 to 3,029,589
_RS14070
RR42_RS14075: RR42_RS14075 - anti-sigma factor, at 3,029,598 to 3,030,227
_RS14075
RR42_RS14080: RR42_RS14080 - RNA polymerase sigma factor RpoE, at 3,030,362 to 3,030,961
_RS14080
RR42_RS14085: RR42_RS14085 - hypothetical protein, at 3,031,091 to 3,031,546
_RS14085
RR42_RS14090: RR42_RS14090 - 3-oxoacyl-ACP synthase, at 3,031,611 to 3,032,843
_RS14090
Position (kb)
3030
3031 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3029.382 kb on - strand at 3029.382 kb on - strand, within RR42_RS14070 at 3029.399 kb on + strand, within RR42_RS14070 at 3029.400 kb on - strand, within RR42_RS14070 at 3029.466 kb on + strand, within RR42_RS14070 at 3029.466 kb on + strand, within RR42_RS14070 at 3029.466 kb on + strand, within RR42_RS14070 at 3029.466 kb on + strand, within RR42_RS14070 at 3029.583 kb on + strand at 3029.583 kb on + strand at 3029.599 kb on + strand at 3029.600 kb on - strand at 3029.600 kb on - strand at 3029.701 kb on + strand, within RR42_RS14075 at 3029.701 kb on + strand, within RR42_RS14075 at 3029.701 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.079 kb on + strand, within RR42_RS14075 at 3030.080 kb on - strand, within RR42_RS14075 at 3030.080 kb on - strand, within RR42_RS14075 at 3030.080 kb on - strand, within RR42_RS14075 at 3030.135 kb on + strand, within RR42_RS14075 at 3030.135 kb on + strand, within RR42_RS14075 at 3030.135 kb on + strand, within RR42_RS14075 at 3030.136 kb on - strand, within RR42_RS14075 at 3030.212 kb on + strand at 3030.212 kb on + strand at 3030.212 kb on + strand at 3030.212 kb on + strand at 3030.213 kb on - strand at 3030.271 kb on + strand at 3030.329 kb on + strand at 3030.330 kb on - strand at 3030.334 kb on - strand at 3030.340 kb on + strand at 3030.346 kb on + strand at 3030.347 kb on - strand at 3030.351 kb on - strand at 3030.351 kb on - strand at 3030.351 kb on - strand at 3030.356 kb on + strand at 3030.357 kb on - strand at 3030.363 kb on + strand at 3030.490 kb on - strand, within RR42_RS14080 at 3030.587 kb on - strand, within RR42_RS14080 at 3030.607 kb on + strand, within RR42_RS14080 at 3030.608 kb on - strand, within RR42_RS14080 at 3030.613 kb on + strand, within RR42_RS14080 at 3030.614 kb on - strand, within RR42_RS14080 at 3030.614 kb on - strand, within RR42_RS14080 at 3030.621 kb on - strand, within RR42_RS14080 at 3030.621 kb on - strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.706 kb on - strand, within RR42_RS14080 at 3030.706 kb on - strand, within RR42_RS14080 at 3030.747 kb on + strand, within RR42_RS14080 at 3030.786 kb on + strand, within RR42_RS14080 at 3030.787 kb on - strand, within RR42_RS14080 at 3030.787 kb on - strand, within RR42_RS14080 at 3030.876 kb on + strand, within RR42_RS14080 at 3030.877 kb on - strand, within RR42_RS14080 at 3030.877 kb on - strand, within RR42_RS14080 at 3030.966 kb on - strand at 3030.966 kb on - strand at 3030.981 kb on + strand at 3030.982 kb on - strand at 3030.982 kb on - strand at 3030.982 kb on - strand at 3031.014 kb on + strand at 3031.024 kb on - strand at 3031.024 kb on - strand at 3031.024 kb on - strand at 3031.024 kb on - strand at 3031.060 kb on + strand at 3031.061 kb on - strand at 3031.061 kb on - strand at 3031.061 kb on - strand at 3031.081 kb on + strand at 3031.082 kb on - strand at 3031.138 kb on + strand, within RR42_RS14085 at 3031.139 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.359 kb on + strand, within RR42_RS14085 at 3031.360 kb on - strand, within RR42_RS14085 at 3031.363 kb on + strand, within RR42_RS14085 at 3031.363 kb on + strand, within RR42_RS14085 at 3031.363 kb on + strand, within RR42_RS14085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,029,382 - +2.1 3,029,382 - RR42_RS14070 0.81 -1.4 3,029,399 + RR42_RS14070 0.82 -1.9 3,029,400 - RR42_RS14070 0.82 -3.2 3,029,466 + RR42_RS14070 0.88 -1.1 3,029,466 + RR42_RS14070 0.88 -0.2 3,029,466 + RR42_RS14070 0.88 -2.2 3,029,466 + RR42_RS14070 0.88 -0.4 3,029,583 + +1.4 3,029,583 + -2.0 3,029,599 + -0.7 3,029,600 - -1.9 3,029,600 - -1.6 3,029,701 + RR42_RS14075 0.16 -0.8 3,029,701 + RR42_RS14075 0.16 -3.3 3,029,701 + RR42_RS14075 0.16 -0.0 3,030,079 + RR42_RS14075 0.76 -0.6 3,030,079 + RR42_RS14075 0.76 -1.1 3,030,079 + RR42_RS14075 0.76 -1.3 3,030,079 + RR42_RS14075 0.76 -0.9 3,030,079 + RR42_RS14075 0.76 -1.9 3,030,079 + RR42_RS14075 0.76 -0.9 3,030,080 - RR42_RS14075 0.77 -0.2 3,030,080 - RR42_RS14075 0.77 +2.1 3,030,080 - RR42_RS14075 0.77 -2.2 3,030,135 + RR42_RS14075 0.85 -0.4 3,030,135 + RR42_RS14075 0.85 -0.7 3,030,135 + RR42_RS14075 0.85 -1.2 3,030,136 - RR42_RS14075 0.85 -2.2 3,030,212 + -1.5 3,030,212 + -2.2 3,030,212 + +2.1 3,030,212 + +0.3 3,030,213 - -0.9 3,030,271 + -2.2 3,030,329 + -0.9 3,030,330 - -1.2 3,030,334 - -1.3 3,030,340 + -3.2 3,030,346 + -1.6 3,030,347 - +0.3 3,030,351 - +0.0 3,030,351 - -2.0 3,030,351 - -0.9 3,030,356 + -1.2 3,030,357 - -0.7 3,030,363 + -1.3 3,030,490 - RR42_RS14080 0.21 -2.6 3,030,587 - RR42_RS14080 0.38 -1.1 3,030,607 + RR42_RS14080 0.41 -2.1 3,030,608 - RR42_RS14080 0.41 -1.8 3,030,613 + RR42_RS14080 0.42 -1.3 3,030,614 - RR42_RS14080 0.42 -1.9 3,030,614 - RR42_RS14080 0.42 -2.9 3,030,621 - RR42_RS14080 0.43 -4.1 3,030,621 - RR42_RS14080 0.43 -3.4 3,030,705 + RR42_RS14080 0.57 -2.9 3,030,705 + RR42_RS14080 0.57 -3.2 3,030,705 + RR42_RS14080 0.57 -2.0 3,030,705 + RR42_RS14080 0.57 -3.9 3,030,706 - RR42_RS14080 0.57 -3.3 3,030,706 - RR42_RS14080 0.57 -3.2 3,030,747 + RR42_RS14080 0.64 -4.6 3,030,786 + RR42_RS14080 0.71 -1.8 3,030,787 - RR42_RS14080 0.71 -1.9 3,030,787 - RR42_RS14080 0.71 -4.6 3,030,876 + RR42_RS14080 0.86 -2.2 3,030,877 - RR42_RS14080 0.86 -3.5 3,030,877 - RR42_RS14080 0.86 -1.8 3,030,966 - -1.2 3,030,966 - -3.6 3,030,981 + -2.5 3,030,982 - +0.3 3,030,982 - +0.6 3,030,982 - -2.0 3,031,014 + +0.6 3,031,024 - +2.0 3,031,024 - +1.6 3,031,024 - +3.4 3,031,024 - +1.0 3,031,060 + -3.7 3,031,061 - +2.3 3,031,061 - +3.4 3,031,061 - +1.3 3,031,081 + -3.2 3,031,082 - +3.3 3,031,138 + RR42_RS14085 0.10 -1.9 3,031,139 - RR42_RS14085 0.11 +1.5 3,031,304 - RR42_RS14085 0.47 +2.8 3,031,304 - RR42_RS14085 0.47 +1.9 3,031,304 - RR42_RS14085 0.47 +1.8 3,031,304 - RR42_RS14085 0.47 +1.3 3,031,359 + RR42_RS14085 0.59 -1.0 3,031,360 - RR42_RS14085 0.59 +1.3 3,031,363 + RR42_RS14085 0.60 -2.2 3,031,363 + RR42_RS14085 0.60 -3.3 3,031,363 + RR42_RS14085 0.60 -1.6
Or see this region's nucleotide sequence