Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14025 and RR42_RS14030 are separated by 30 nucleotides RR42_RS14030 and RR42_RS14035 overlap by 8 nucleotides RR42_RS14035 and RR42_RS14040 overlap by 4 nucleotides RR42_RS14040 and RR42_RS14045 are separated by 1 nucleotides
RR42_RS14025: RR42_RS14025 - pyridoxine 5'-phosphate synthase, at 3,019,647 to 3,020,429
_RS14025
RR42_RS14030: RR42_RS14030 - DNA recombination protein RecO, at 3,020,460 to 3,021,338
_RS14030
RR42_RS14035: RR42_RS14035 - GTPase Era, at 3,021,331 to 3,022,266
_RS14035
RR42_RS14040: RR42_RS14040 - ribonuclease III, at 3,022,263 to 3,023,033
_RS14040
RR42_RS14045: RR42_RS14045 - membrane protein, at 3,023,035 to 3,023,391
_RS14045
Position (kb)
3021
3022
3023 Strain fitness (log2 ratio)
-1
0
1
2 at 3020.443 kb on - strand at 3020.457 kb on - strand at 3020.519 kb on - strand at 3020.577 kb on - strand, within RR42_RS14030 at 3020.578 kb on + strand, within RR42_RS14030 at 3020.579 kb on - strand, within RR42_RS14030 at 3020.677 kb on + strand, within RR42_RS14030 at 3020.683 kb on + strand, within RR42_RS14030 at 3020.683 kb on + strand, within RR42_RS14030 at 3020.834 kb on - strand, within RR42_RS14030 at 3020.891 kb on - strand, within RR42_RS14030 at 3021.024 kb on + strand, within RR42_RS14030 at 3023.055 kb on - strand at 3023.059 kb on - strand at 3023.082 kb on - strand, within RR42_RS14045 at 3023.082 kb on - strand, within RR42_RS14045 at 3023.084 kb on - strand, within RR42_RS14045 at 3023.179 kb on - strand, within RR42_RS14045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 3,020,443 - +1.1 3,020,457 - +0.3 3,020,519 - -0.3 3,020,577 - RR42_RS14030 0.13 -0.2 3,020,578 + RR42_RS14030 0.13 +1.7 3,020,579 - RR42_RS14030 0.14 -1.2 3,020,677 + RR42_RS14030 0.25 -1.2 3,020,683 + RR42_RS14030 0.25 -0.3 3,020,683 + RR42_RS14030 0.25 +0.7 3,020,834 - RR42_RS14030 0.43 -0.1 3,020,891 - RR42_RS14030 0.49 +1.8 3,021,024 + RR42_RS14030 0.64 -0.5 3,023,055 - +0.7 3,023,059 - +0.0 3,023,082 - RR42_RS14045 0.13 -0.3 3,023,082 - RR42_RS14045 0.13 -1.0 3,023,084 - RR42_RS14045 0.14 -0.8 3,023,179 - RR42_RS14045 0.40 +0.2
Or see this region's nucleotide sequence