Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11460 and RR42_RS11465 overlap by 4 nucleotides RR42_RS11465 and RR42_RS11470 overlap by 4 nucleotides RR42_RS11470 and RR42_RS11475 overlap by 29 nucleotides RR42_RS11475 and RR42_RS11480 are separated by 220 nucleotides RR42_RS11480 and RR42_RS11485 are separated by 128 nucleotides
RR42_RS11460: RR42_RS11460 - pyrroloquinoline quinone biosynthesis protein PqqB, at 2,452,592 to 2,453,509
_RS11460
RR42_RS11465: RR42_RS11465 - pyrroloquinoline quinone biosynthesis protein PqqC, at 2,453,506 to 2,454,198
_RS11465
RR42_RS11470: RR42_RS11470 - pyrroloquinoline quinone biosynthesis protein PqqD, at 2,454,195 to 2,454,467
_RS11470
RR42_RS11475: RR42_RS11475 - pyrroloquinoline quinone biosynthesis protein PqqE, at 2,454,439 to 2,455,596
_RS11475
RR42_RS11480: RR42_RS11480 - cytochrome C, at 2,455,817 to 2,456,134
_RS11480
RR42_RS11485: RR42_RS11485 - dehydrogenase, at 2,456,263 to 2,458,005
_RS11485
Position (kb)
2454
2455
2456 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2453.659 kb on + strand, within RR42_RS11465 at 2453.662 kb on + strand, within RR42_RS11465 at 2453.663 kb on - strand, within RR42_RS11465 at 2453.663 kb on - strand, within RR42_RS11465 at 2453.663 kb on - strand, within RR42_RS11465 at 2453.663 kb on - strand, within RR42_RS11465 at 2453.887 kb on + strand, within RR42_RS11465 at 2453.888 kb on - strand, within RR42_RS11465 at 2453.888 kb on - strand, within RR42_RS11465 at 2453.999 kb on - strand, within RR42_RS11465 at 2454.422 kb on - strand, within RR42_RS11470 at 2454.503 kb on + strand at 2454.571 kb on + strand, within RR42_RS11475 at 2454.731 kb on - strand, within RR42_RS11475 at 2454.808 kb on - strand, within RR42_RS11475 at 2454.815 kb on + strand, within RR42_RS11475 at 2455.006 kb on + strand, within RR42_RS11475 at 2455.007 kb on - strand, within RR42_RS11475 at 2455.031 kb on - strand, within RR42_RS11475 at 2455.034 kb on - strand, within RR42_RS11475 at 2455.316 kb on + strand, within RR42_RS11475 at 2455.317 kb on - strand, within RR42_RS11475 at 2455.511 kb on + strand at 2455.784 kb on + strand at 2455.968 kb on + strand, within RR42_RS11480 at 2455.969 kb on - strand, within RR42_RS11480 at 2456.003 kb on + strand, within RR42_RS11480 at 2456.155 kb on + strand at 2456.156 kb on - strand at 2456.156 kb on - strand at 2456.159 kb on + strand at 2456.160 kb on - strand at 2456.238 kb on + strand at 2456.238 kb on + strand at 2456.238 kb on + strand at 2456.238 kb on + strand at 2456.239 kb on - strand at 2456.239 kb on - strand at 2456.243 kb on + strand at 2456.243 kb on + strand at 2456.243 kb on + strand at 2456.244 kb on - strand at 2456.384 kb on + strand at 2456.385 kb on - strand at 2456.385 kb on - strand at 2456.489 kb on + strand, within RR42_RS11485 at 2456.559 kb on - strand, within RR42_RS11485
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,453,659 + RR42_RS11465 0.22 -1.0 2,453,662 + RR42_RS11465 0.23 -0.5 2,453,663 - RR42_RS11465 0.23 -0.2 2,453,663 - RR42_RS11465 0.23 -0.4 2,453,663 - RR42_RS11465 0.23 +0.5 2,453,663 - RR42_RS11465 0.23 -0.2 2,453,887 + RR42_RS11465 0.55 +0.6 2,453,888 - RR42_RS11465 0.55 -0.0 2,453,888 - RR42_RS11465 0.55 -0.2 2,453,999 - RR42_RS11465 0.71 +0.7 2,454,422 - RR42_RS11470 0.83 +1.6 2,454,503 + -1.0 2,454,571 + RR42_RS11475 0.11 -0.5 2,454,731 - RR42_RS11475 0.25 +0.1 2,454,808 - RR42_RS11475 0.32 -0.6 2,454,815 + RR42_RS11475 0.32 -0.9 2,455,006 + RR42_RS11475 0.49 -0.7 2,455,007 - RR42_RS11475 0.49 +0.4 2,455,031 - RR42_RS11475 0.51 +0.8 2,455,034 - RR42_RS11475 0.51 +0.5 2,455,316 + RR42_RS11475 0.76 -0.3 2,455,317 - RR42_RS11475 0.76 -1.0 2,455,511 + -0.7 2,455,784 + +1.5 2,455,968 + RR42_RS11480 0.47 -0.8 2,455,969 - RR42_RS11480 0.48 +0.8 2,456,003 + RR42_RS11480 0.58 -1.5 2,456,155 + +0.8 2,456,156 - +0.5 2,456,156 - +2.9 2,456,159 + +1.1 2,456,160 - -0.3 2,456,238 + +1.3 2,456,238 + -1.2 2,456,238 + +0.5 2,456,238 + -1.0 2,456,239 - -0.3 2,456,239 - -0.5 2,456,243 + -1.3 2,456,243 + -2.1 2,456,243 + +0.1 2,456,244 - -0.1 2,456,384 + -2.7 2,456,385 - +1.0 2,456,385 - +0.9 2,456,489 + RR42_RS11485 0.13 -0.1 2,456,559 - RR42_RS11485 0.17 +0.0
Or see this region's nucleotide sequence