Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11445 and RR42_RS11450 are separated by 28 nucleotides RR42_RS11450 and RR42_RS11455 are separated by 41 nucleotides RR42_RS11455 and RR42_RS11460 are separated by 272 nucleotides
RR42_RS11445: RR42_RS11445 - Fis family transcriptional regulator, at 2,448,363 to 2,450,402
_RS11445
RR42_RS11450: RR42_RS11450 - MBL fold metallo-hydrolase, at 2,450,431 to 2,451,348
_RS11450
RR42_RS11455: RR42_RS11455 - prolyl aminopeptidase, at 2,451,390 to 2,452,319
_RS11455
RR42_RS11460: RR42_RS11460 - pyrroloquinoline quinone biosynthesis protein PqqB, at 2,452,592 to 2,453,509
_RS11460
Position (kb)
2451
2452
2453 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2450.516 kb on + strand at 2450.689 kb on + strand, within RR42_RS11450 at 2450.785 kb on + strand, within RR42_RS11450 at 2450.786 kb on - strand, within RR42_RS11450 at 2450.786 kb on - strand, within RR42_RS11450 at 2450.807 kb on + strand, within RR42_RS11450 at 2450.807 kb on + strand, within RR42_RS11450 at 2450.807 kb on + strand, within RR42_RS11450 at 2450.808 kb on - strand, within RR42_RS11450 at 2450.808 kb on - strand, within RR42_RS11450 at 2450.817 kb on + strand, within RR42_RS11450 at 2450.830 kb on - strand, within RR42_RS11450 at 2451.275 kb on + strand at 2451.275 kb on + strand at 2451.275 kb on + strand at 2451.275 kb on + strand at 2451.275 kb on + strand at 2451.276 kb on - strand at 2451.276 kb on - strand at 2451.276 kb on - strand at 2451.276 kb on - strand at 2451.276 kb on - strand at 2451.276 kb on - strand at 2451.658 kb on + strand, within RR42_RS11455 at 2451.783 kb on - strand, within RR42_RS11455 at 2451.973 kb on + strand, within RR42_RS11455 at 2451.974 kb on - strand, within RR42_RS11455 at 2452.379 kb on + strand at 2452.379 kb on + strand at 2452.383 kb on + strand at 2452.383 kb on + strand at 2452.383 kb on + strand at 2452.383 kb on + strand at 2452.383 kb on + strand at 2452.384 kb on - strand at 2452.384 kb on - strand at 2452.384 kb on - strand at 2452.384 kb on - strand at 2452.384 kb on - strand at 2452.437 kb on - strand at 2452.462 kb on + strand at 2452.463 kb on - strand at 2452.463 kb on - strand at 2452.463 kb on - strand at 2452.584 kb on + strand at 2452.599 kb on + strand at 2452.599 kb on + strand at 2452.599 kb on + strand at 2452.600 kb on - strand at 2452.923 kb on - strand, within RR42_RS11460 at 2452.988 kb on + strand, within RR42_RS11460 at 2452.989 kb on - strand, within RR42_RS11460 at 2452.989 kb on - strand, within RR42_RS11460 at 2453.100 kb on - strand, within RR42_RS11460 at 2453.183 kb on + strand, within RR42_RS11460 at 2453.183 kb on + strand, within RR42_RS11460 at 2453.184 kb on - strand, within RR42_RS11460 at 2453.184 kb on - strand, within RR42_RS11460 at 2453.244 kb on + strand, within RR42_RS11460 at 2453.245 kb on - strand, within RR42_RS11460 at 2453.245 kb on - strand, within RR42_RS11460 at 2453.245 kb on - strand, within RR42_RS11460 at 2453.245 kb on - strand, within RR42_RS11460 at 2453.245 kb on - strand, within RR42_RS11460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,450,516 + +0.0 2,450,689 + RR42_RS11450 0.28 -0.5 2,450,785 + RR42_RS11450 0.39 -0.5 2,450,786 - RR42_RS11450 0.39 +0.2 2,450,786 - RR42_RS11450 0.39 +0.6 2,450,807 + RR42_RS11450 0.41 +0.6 2,450,807 + RR42_RS11450 0.41 +0.3 2,450,807 + RR42_RS11450 0.41 -0.5 2,450,808 - RR42_RS11450 0.41 -1.2 2,450,808 - RR42_RS11450 0.41 +2.2 2,450,817 + RR42_RS11450 0.42 +0.9 2,450,830 - RR42_RS11450 0.43 +0.0 2,451,275 + +0.2 2,451,275 + -0.9 2,451,275 + -1.0 2,451,275 + -0.2 2,451,275 + +0.1 2,451,276 - +0.4 2,451,276 - -0.1 2,451,276 - +0.3 2,451,276 - +0.2 2,451,276 - -1.0 2,451,276 - +0.4 2,451,658 + RR42_RS11455 0.29 +0.7 2,451,783 - RR42_RS11455 0.42 +0.9 2,451,973 + RR42_RS11455 0.63 -0.0 2,451,974 - RR42_RS11455 0.63 +0.2 2,452,379 + -0.0 2,452,379 + +0.1 2,452,383 + +1.2 2,452,383 + +0.9 2,452,383 + +0.1 2,452,383 + -0.6 2,452,383 + -0.7 2,452,384 - -0.0 2,452,384 - +0.5 2,452,384 - -0.0 2,452,384 - +0.5 2,452,384 - -0.2 2,452,437 - +0.6 2,452,462 + +0.6 2,452,463 - -0.3 2,452,463 - -1.1 2,452,463 - -0.1 2,452,584 + -0.0 2,452,599 + +0.8 2,452,599 + +0.0 2,452,599 + +0.9 2,452,600 - +1.3 2,452,923 - RR42_RS11460 0.36 +0.7 2,452,988 + RR42_RS11460 0.43 -1.3 2,452,989 - RR42_RS11460 0.43 +1.2 2,452,989 - RR42_RS11460 0.43 +0.5 2,453,100 - RR42_RS11460 0.55 +0.5 2,453,183 + RR42_RS11460 0.64 +1.2 2,453,183 + RR42_RS11460 0.64 +0.5 2,453,184 - RR42_RS11460 0.64 +1.0 2,453,184 - RR42_RS11460 0.64 +0.4 2,453,244 + RR42_RS11460 0.71 +1.4 2,453,245 - RR42_RS11460 0.71 -1.5 2,453,245 - RR42_RS11460 0.71 -0.0 2,453,245 - RR42_RS11460 0.71 -2.1 2,453,245 - RR42_RS11460 0.71 +0.6 2,453,245 - RR42_RS11460 0.71 -0.7
Or see this region's nucleotide sequence