Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11130 and RR42_RS11135 overlap by 1 nucleotides RR42_RS11135 and RR42_RS11140 overlap by 4 nucleotides RR42_RS11140 and RR42_RS11145 are separated by 10 nucleotides
RR42_RS11130: RR42_RS11130 - mannosyltransferase, at 2,377,293 to 2,378,006
_RS11130
RR42_RS11135: RR42_RS11135 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,378,006 to 2,378,779
_RS11135
RR42_RS11140: RR42_RS11140 - ABC transporter permease, at 2,378,776 to 2,380,089
_RS11140
RR42_RS11145: RR42_RS11145 - ABC transporter permease, at 2,380,100 to 2,381,047
_RS11145
Position (kb)
2378
2379
2380
2381 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2377.925 kb on + strand, within RR42_RS11130 at 2377.950 kb on - strand at 2377.967 kb on - strand at 2378.052 kb on + strand at 2378.331 kb on + strand, within RR42_RS11135 at 2378.332 kb on - strand, within RR42_RS11135 at 2378.356 kb on + strand, within RR42_RS11135 at 2378.357 kb on - strand, within RR42_RS11135 at 2378.445 kb on + strand, within RR42_RS11135 at 2378.446 kb on - strand, within RR42_RS11135 at 2378.512 kb on + strand, within RR42_RS11135 at 2378.512 kb on + strand, within RR42_RS11135 at 2378.513 kb on - strand, within RR42_RS11135 at 2378.654 kb on - strand, within RR42_RS11135 at 2378.654 kb on - strand, within RR42_RS11135 at 2378.750 kb on + strand at 2378.751 kb on - strand at 2378.751 kb on - strand at 2378.807 kb on - strand at 2378.972 kb on + strand, within RR42_RS11140 at 2379.038 kb on + strand, within RR42_RS11140 at 2379.155 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.482 kb on + strand, within RR42_RS11140 at 2379.483 kb on - strand, within RR42_RS11140 at 2379.483 kb on - strand, within RR42_RS11140 at 2379.483 kb on - strand, within RR42_RS11140 at 2379.524 kb on + strand, within RR42_RS11140 at 2379.527 kb on + strand, within RR42_RS11140 at 2379.528 kb on - strand, within RR42_RS11140 at 2379.594 kb on + strand, within RR42_RS11140 at 2379.708 kb on - strand, within RR42_RS11140 at 2379.799 kb on - strand, within RR42_RS11140 at 2379.799 kb on - strand, within RR42_RS11140 at 2379.890 kb on + strand, within RR42_RS11140 at 2379.890 kb on + strand, within RR42_RS11140 at 2379.906 kb on + strand, within RR42_RS11140 at 2379.907 kb on - strand, within RR42_RS11140 at 2380.098 kb on + strand at 2380.098 kb on + strand at 2380.099 kb on - strand at 2380.206 kb on - strand, within RR42_RS11145 at 2380.338 kb on - strand, within RR42_RS11145 at 2380.415 kb on - strand, within RR42_RS11145 at 2380.415 kb on - strand, within RR42_RS11145 at 2380.415 kb on - strand, within RR42_RS11145 at 2380.499 kb on - strand, within RR42_RS11145 at 2380.582 kb on - strand, within RR42_RS11145 at 2380.582 kb on - strand, within RR42_RS11145 at 2380.653 kb on - strand, within RR42_RS11145 at 2380.716 kb on - strand, within RR42_RS11145 at 2380.743 kb on + strand, within RR42_RS11145 at 2380.759 kb on + strand, within RR42_RS11145 at 2380.760 kb on - strand, within RR42_RS11145 at 2380.785 kb on + strand, within RR42_RS11145 at 2380.785 kb on + strand, within RR42_RS11145 at 2380.785 kb on + strand, within RR42_RS11145 at 2380.785 kb on + strand, within RR42_RS11145 at 2380.918 kb on + strand, within RR42_RS11145 at 2380.918 kb on + strand, within RR42_RS11145 at 2380.918 kb on + strand, within RR42_RS11145 at 2380.919 kb on - strand at 2380.920 kb on + strand, within RR42_RS11145 at 2380.920 kb on + strand, within RR42_RS11145 at 2380.920 kb on + strand, within RR42_RS11145 at 2380.920 kb on + strand, within RR42_RS11145 at 2380.921 kb on - strand, within RR42_RS11145 at 2380.921 kb on - strand, within RR42_RS11145 at 2381.008 kb on - strand at 2381.052 kb on + strand at 2381.052 kb on + strand at 2381.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,377,925 + RR42_RS11130 0.89 -0.6 2,377,950 - -0.2 2,377,967 - +0.5 2,378,052 + +0.3 2,378,331 + RR42_RS11135 0.42 -0.8 2,378,332 - RR42_RS11135 0.42 -0.5 2,378,356 + RR42_RS11135 0.45 +1.1 2,378,357 - RR42_RS11135 0.45 +0.4 2,378,445 + RR42_RS11135 0.57 -1.2 2,378,446 - RR42_RS11135 0.57 +0.0 2,378,512 + RR42_RS11135 0.65 +0.4 2,378,512 + RR42_RS11135 0.65 -1.0 2,378,513 - RR42_RS11135 0.66 +0.3 2,378,654 - RR42_RS11135 0.84 +0.1 2,378,654 - RR42_RS11135 0.84 -0.2 2,378,750 + -1.8 2,378,751 - +0.1 2,378,751 - -0.8 2,378,807 - -0.0 2,378,972 + RR42_RS11140 0.15 +0.7 2,379,038 + RR42_RS11140 0.20 +1.3 2,379,155 + RR42_RS11140 0.29 -0.4 2,379,482 + RR42_RS11140 0.54 +0.0 2,379,482 + RR42_RS11140 0.54 -0.7 2,379,482 + RR42_RS11140 0.54 +0.3 2,379,482 + RR42_RS11140 0.54 +0.1 2,379,483 - RR42_RS11140 0.54 -0.3 2,379,483 - RR42_RS11140 0.54 -0.7 2,379,483 - RR42_RS11140 0.54 +0.4 2,379,524 + RR42_RS11140 0.57 -2.5 2,379,527 + RR42_RS11140 0.57 -0.3 2,379,528 - RR42_RS11140 0.57 -0.1 2,379,594 + RR42_RS11140 0.62 -0.6 2,379,708 - RR42_RS11140 0.71 +1.0 2,379,799 - RR42_RS11140 0.78 -0.8 2,379,799 - RR42_RS11140 0.78 -0.3 2,379,890 + RR42_RS11140 0.85 -0.3 2,379,890 + RR42_RS11140 0.85 -0.8 2,379,906 + RR42_RS11140 0.86 -0.4 2,379,907 - RR42_RS11140 0.86 -0.5 2,380,098 + +0.3 2,380,098 + -0.4 2,380,099 - -0.3 2,380,206 - RR42_RS11145 0.11 -1.0 2,380,338 - RR42_RS11145 0.25 +0.6 2,380,415 - RR42_RS11145 0.33 +1.5 2,380,415 - RR42_RS11145 0.33 +1.3 2,380,415 - RR42_RS11145 0.33 +0.6 2,380,499 - RR42_RS11145 0.42 +0.6 2,380,582 - RR42_RS11145 0.51 +0.1 2,380,582 - RR42_RS11145 0.51 -1.7 2,380,653 - RR42_RS11145 0.58 -0.2 2,380,716 - RR42_RS11145 0.65 -1.0 2,380,743 + RR42_RS11145 0.68 +0.5 2,380,759 + RR42_RS11145 0.70 +1.3 2,380,760 - RR42_RS11145 0.70 -0.1 2,380,785 + RR42_RS11145 0.72 -0.2 2,380,785 + RR42_RS11145 0.72 -0.1 2,380,785 + RR42_RS11145 0.72 -0.3 2,380,785 + RR42_RS11145 0.72 -1.0 2,380,918 + RR42_RS11145 0.86 +0.2 2,380,918 + RR42_RS11145 0.86 +0.2 2,380,918 + RR42_RS11145 0.86 -1.4 2,380,919 - +2.1 2,380,920 + RR42_RS11145 0.86 -0.4 2,380,920 + RR42_RS11145 0.86 -0.3 2,380,920 + RR42_RS11145 0.86 +0.6 2,380,920 + RR42_RS11145 0.86 +0.5 2,380,921 - RR42_RS11145 0.87 +1.2 2,380,921 - RR42_RS11145 0.87 -0.1 2,381,008 - +0.1 2,381,052 + +0.5 2,381,052 + -2.3 2,381,053 - +0.2
Or see this region's nucleotide sequence