Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10770 and RR42_RS10775 are separated by 37 nucleotides RR42_RS10775 and RR42_RS10780 are separated by 138 nucleotides RR42_RS10780 and RR42_RS10785 are separated by 190 nucleotides
RR42_RS10770: RR42_RS10770 - three-component membrane-bound alcohol deshydrogenase (adha), at 2,301,678 to 2,303,546
_RS10770
RR42_RS10775: RR42_RS10775 - alcohol dehydrogenase, at 2,303,584 to 2,304,888
_RS10775
RR42_RS10780: RR42_RS10780 - alcohol dehydrogenase, at 2,305,027 to 2,305,212
_RS10780
RR42_RS10785: RR42_RS10785 - GntR family transcriptional regulator, at 2,305,403 to 2,306,818
_RS10785
Position (kb)
2303
2304
2305 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2302.666 kb on + strand, within RR42_RS10770 at 2302.667 kb on - strand, within RR42_RS10770 at 2302.667 kb on - strand, within RR42_RS10770 at 2302.667 kb on - strand, within RR42_RS10770 at 2302.667 kb on - strand, within RR42_RS10770 at 2302.744 kb on + strand, within RR42_RS10770 at 2302.745 kb on - strand, within RR42_RS10770 at 2302.745 kb on - strand, within RR42_RS10770 at 2302.762 kb on + strand, within RR42_RS10770 at 2302.923 kb on + strand, within RR42_RS10770 at 2302.923 kb on + strand, within RR42_RS10770 at 2302.923 kb on + strand, within RR42_RS10770 at 2302.923 kb on + strand, within RR42_RS10770 at 2302.923 kb on + strand, within RR42_RS10770 at 2302.924 kb on - strand, within RR42_RS10770 at 2302.924 kb on - strand, within RR42_RS10770 at 2302.924 kb on - strand, within RR42_RS10770 at 2302.938 kb on + strand, within RR42_RS10770 at 2302.938 kb on + strand, within RR42_RS10770 at 2302.939 kb on - strand, within RR42_RS10770 at 2303.051 kb on - strand, within RR42_RS10770 at 2303.164 kb on + strand, within RR42_RS10770 at 2303.164 kb on + strand, within RR42_RS10770 at 2303.165 kb on - strand, within RR42_RS10770 at 2303.193 kb on - strand, within RR42_RS10770 at 2303.278 kb on + strand, within RR42_RS10770 at 2303.359 kb on + strand, within RR42_RS10770 at 2303.359 kb on + strand, within RR42_RS10770 at 2303.396 kb on + strand at 2303.397 kb on - strand at 2303.397 kb on - strand at 2303.398 kb on + strand at 2303.398 kb on + strand at 2303.399 kb on - strand at 2303.399 kb on - strand at 2303.399 kb on - strand at 2303.399 kb on - strand at 2303.472 kb on + strand at 2303.472 kb on + strand at 2303.472 kb on + strand at 2303.472 kb on + strand at 2303.473 kb on - strand at 2303.552 kb on + strand at 2303.553 kb on - strand at 2303.553 kb on - strand at 2303.553 kb on - strand at 2303.553 kb on - strand at 2303.553 kb on - strand at 2303.568 kb on + strand at 2303.568 kb on + strand at 2303.568 kb on + strand at 2303.750 kb on + strand, within RR42_RS10775 at 2303.919 kb on - strand, within RR42_RS10775 at 2303.981 kb on + strand, within RR42_RS10775 at 2303.987 kb on + strand, within RR42_RS10775 at 2303.987 kb on + strand, within RR42_RS10775 at 2303.988 kb on - strand, within RR42_RS10775 at 2303.988 kb on - strand, within RR42_RS10775 at 2304.029 kb on + strand, within RR42_RS10775 at 2304.029 kb on + strand, within RR42_RS10775 at 2304.029 kb on + strand, within RR42_RS10775 at 2304.133 kb on - strand, within RR42_RS10775 at 2304.141 kb on - strand, within RR42_RS10775 at 2304.295 kb on - strand, within RR42_RS10775 at 2304.295 kb on - strand, within RR42_RS10775 at 2304.332 kb on + strand, within RR42_RS10775 at 2304.332 kb on + strand, within RR42_RS10775 at 2304.332 kb on + strand, within RR42_RS10775 at 2304.497 kb on + strand, within RR42_RS10775 at 2304.498 kb on - strand, within RR42_RS10775 at 2304.498 kb on - strand, within RR42_RS10775 at 2304.503 kb on + strand, within RR42_RS10775 at 2304.504 kb on - strand, within RR42_RS10775 at 2304.506 kb on - strand, within RR42_RS10775 at 2304.506 kb on - strand, within RR42_RS10775 at 2304.548 kb on + strand, within RR42_RS10775 at 2304.563 kb on + strand, within RR42_RS10775 at 2304.672 kb on - strand, within RR42_RS10775 at 2304.838 kb on + strand at 2305.225 kb on + strand at 2305.225 kb on + strand at 2305.225 kb on + strand at 2305.225 kb on + strand at 2305.226 kb on - strand at 2305.226 kb on - strand at 2305.226 kb on - strand at 2305.226 kb on - strand at 2305.273 kb on + strand at 2305.273 kb on + strand at 2305.274 kb on - strand at 2305.274 kb on - strand at 2305.287 kb on + strand at 2305.288 kb on - strand at 2305.288 kb on - strand at 2305.541 kb on + strand at 2305.541 kb on + strand at 2305.541 kb on + strand at 2305.542 kb on - strand at 2305.542 kb on - strand at 2305.595 kb on + strand, within RR42_RS10785 at 2305.595 kb on + strand, within RR42_RS10785 at 2305.596 kb on - strand, within RR42_RS10785 at 2305.760 kb on + strand, within RR42_RS10785 at 2305.760 kb on + strand, within RR42_RS10785 at 2305.760 kb on + strand, within RR42_RS10785 at 2305.760 kb on + strand, within RR42_RS10785 at 2305.820 kb on + strand, within RR42_RS10785 at 2305.821 kb on - strand, within RR42_RS10785 at 2305.880 kb on + strand, within RR42_RS10785
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,302,666 + RR42_RS10770 0.53 +0.4 2,302,667 - RR42_RS10770 0.53 +0.5 2,302,667 - RR42_RS10770 0.53 +0.3 2,302,667 - RR42_RS10770 0.53 +0.6 2,302,667 - RR42_RS10770 0.53 -0.1 2,302,744 + RR42_RS10770 0.57 -1.2 2,302,745 - RR42_RS10770 0.57 +0.5 2,302,745 - RR42_RS10770 0.57 +0.6 2,302,762 + RR42_RS10770 0.58 -0.1 2,302,923 + RR42_RS10770 0.67 +0.2 2,302,923 + RR42_RS10770 0.67 +1.0 2,302,923 + RR42_RS10770 0.67 +0.5 2,302,923 + RR42_RS10770 0.67 -0.5 2,302,923 + RR42_RS10770 0.67 -0.6 2,302,924 - RR42_RS10770 0.67 +0.1 2,302,924 - RR42_RS10770 0.67 -0.5 2,302,924 - RR42_RS10770 0.67 -0.1 2,302,938 + RR42_RS10770 0.67 +0.0 2,302,938 + RR42_RS10770 0.67 -0.0 2,302,939 - RR42_RS10770 0.67 +0.0 2,303,051 - RR42_RS10770 0.73 +0.2 2,303,164 + RR42_RS10770 0.80 +1.4 2,303,164 + RR42_RS10770 0.80 -0.1 2,303,165 - RR42_RS10770 0.80 +1.0 2,303,193 - RR42_RS10770 0.81 -0.2 2,303,278 + RR42_RS10770 0.86 -1.0 2,303,359 + RR42_RS10770 0.90 -0.4 2,303,359 + RR42_RS10770 0.90 +1.7 2,303,396 + +1.3 2,303,397 - -0.7 2,303,397 - -0.6 2,303,398 + -0.4 2,303,398 + -1.8 2,303,399 - +1.5 2,303,399 - -1.0 2,303,399 - +0.5 2,303,399 - -0.5 2,303,472 + -0.8 2,303,472 + -0.6 2,303,472 + -0.3 2,303,472 + -0.3 2,303,473 - +1.0 2,303,552 + -2.9 2,303,553 - -0.7 2,303,553 - -0.2 2,303,553 - +0.4 2,303,553 - -0.9 2,303,553 - -0.6 2,303,568 + -0.3 2,303,568 + -0.3 2,303,568 + -0.5 2,303,750 + RR42_RS10775 0.13 +0.6 2,303,919 - RR42_RS10775 0.26 +0.1 2,303,981 + RR42_RS10775 0.30 +0.2 2,303,987 + RR42_RS10775 0.31 +1.5 2,303,987 + RR42_RS10775 0.31 +1.1 2,303,988 - RR42_RS10775 0.31 -0.5 2,303,988 - RR42_RS10775 0.31 -0.7 2,304,029 + RR42_RS10775 0.34 +0.4 2,304,029 + RR42_RS10775 0.34 +0.6 2,304,029 + RR42_RS10775 0.34 -0.3 2,304,133 - RR42_RS10775 0.42 -0.4 2,304,141 - RR42_RS10775 0.43 +1.1 2,304,295 - RR42_RS10775 0.54 +0.5 2,304,295 - RR42_RS10775 0.54 +0.5 2,304,332 + RR42_RS10775 0.57 +0.1 2,304,332 + RR42_RS10775 0.57 -0.1 2,304,332 + RR42_RS10775 0.57 +1.5 2,304,497 + RR42_RS10775 0.70 +1.0 2,304,498 - RR42_RS10775 0.70 +0.9 2,304,498 - RR42_RS10775 0.70 +0.2 2,304,503 + RR42_RS10775 0.70 +0.4 2,304,504 - RR42_RS10775 0.70 -0.6 2,304,506 - RR42_RS10775 0.71 +0.5 2,304,506 - RR42_RS10775 0.71 -1.0 2,304,548 + RR42_RS10775 0.74 -0.2 2,304,563 + RR42_RS10775 0.75 -0.3 2,304,672 - RR42_RS10775 0.83 -0.3 2,304,838 + +2.0 2,305,225 + -0.3 2,305,225 + -2.4 2,305,225 + +0.8 2,305,225 + +0.1 2,305,226 - -0.1 2,305,226 - +0.3 2,305,226 - +0.4 2,305,226 - +2.1 2,305,273 + +0.5 2,305,273 + +0.8 2,305,274 - +0.3 2,305,274 - +0.5 2,305,287 + +1.1 2,305,288 - -1.2 2,305,288 - +0.4 2,305,541 + +0.1 2,305,541 + -0.3 2,305,541 + +0.7 2,305,542 - -0.8 2,305,542 - -0.7 2,305,595 + RR42_RS10785 0.14 +0.7 2,305,595 + RR42_RS10785 0.14 -1.1 2,305,596 - RR42_RS10785 0.14 +0.4 2,305,760 + RR42_RS10785 0.25 -0.2 2,305,760 + RR42_RS10785 0.25 +1.2 2,305,760 + RR42_RS10785 0.25 +0.9 2,305,760 + RR42_RS10785 0.25 -0.4 2,305,820 + RR42_RS10785 0.29 +0.2 2,305,821 - RR42_RS10785 0.30 -0.4 2,305,880 + RR42_RS10785 0.34 -0.0
Or see this region's nucleotide sequence