Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10210 and RR42_RS10215 are separated by 474 nucleotides RR42_RS10215 and RR42_RS10220 are separated by 170 nucleotides RR42_RS10220 and RR42_RS10225 are separated by 18 nucleotides
RR42_RS10210: RR42_RS10210 - alcohol dehydrogenase, at 2,184,688 to 2,185,671
_RS10210
RR42_RS10215: RR42_RS10215 - N-ethylmaleimide reductase, at 2,186,146 to 2,187,255
_RS10215
RR42_RS10220: RR42_RS10220 - hypothetical protein, at 2,187,426 to 2,187,908
_RS10220
RR42_RS10225: RR42_RS10225 - nitronate monooxygenase, at 2,187,927 to 2,188,907
_RS10225
Position (kb)
2186
2187
2188 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2185.388 kb on + strand, within RR42_RS10210 at 2185.388 kb on + strand, within RR42_RS10210 at 2185.388 kb on + strand, within RR42_RS10210 at 2185.389 kb on - strand, within RR42_RS10210 at 2185.389 kb on - strand, within RR42_RS10210 at 2185.463 kb on - strand, within RR42_RS10210 at 2185.507 kb on + strand, within RR42_RS10210 at 2185.547 kb on + strand, within RR42_RS10210 at 2185.547 kb on + strand, within RR42_RS10210 at 2185.654 kb on + strand at 2185.841 kb on + strand at 2185.858 kb on - strand at 2185.858 kb on - strand at 2185.884 kb on + strand at 2185.887 kb on + strand at 2185.888 kb on - strand at 2185.888 kb on - strand at 2186.045 kb on + strand at 2186.046 kb on - strand at 2186.046 kb on - strand at 2186.268 kb on + strand, within RR42_RS10215 at 2186.268 kb on + strand, within RR42_RS10215 at 2186.269 kb on - strand, within RR42_RS10215 at 2186.448 kb on + strand, within RR42_RS10215 at 2186.448 kb on + strand, within RR42_RS10215 at 2186.449 kb on - strand, within RR42_RS10215 at 2186.450 kb on + strand, within RR42_RS10215 at 2186.450 kb on + strand, within RR42_RS10215 at 2186.450 kb on + strand, within RR42_RS10215 at 2186.451 kb on - strand, within RR42_RS10215 at 2186.451 kb on - strand, within RR42_RS10215 at 2186.451 kb on - strand, within RR42_RS10215 at 2186.640 kb on - strand, within RR42_RS10215 at 2186.822 kb on + strand, within RR42_RS10215 at 2186.841 kb on - strand, within RR42_RS10215 at 2186.876 kb on - strand, within RR42_RS10215 at 2187.011 kb on + strand, within RR42_RS10215 at 2187.052 kb on + strand, within RR42_RS10215 at 2187.052 kb on + strand, within RR42_RS10215 at 2187.101 kb on + strand, within RR42_RS10215 at 2187.101 kb on + strand, within RR42_RS10215 at 2187.102 kb on - strand, within RR42_RS10215 at 2187.105 kb on - strand, within RR42_RS10215 at 2187.259 kb on + strand at 2187.259 kb on + strand at 2187.259 kb on + strand at 2187.265 kb on - strand at 2187.614 kb on + strand, within RR42_RS10220 at 2187.615 kb on - strand, within RR42_RS10220 at 2187.615 kb on - strand, within RR42_RS10220 at 2187.615 kb on - strand, within RR42_RS10220 at 2187.618 kb on + strand, within RR42_RS10220 at 2187.743 kb on + strand, within RR42_RS10220 at 2187.745 kb on + strand, within RR42_RS10220 at 2187.764 kb on - strand, within RR42_RS10220 at 2187.884 kb on + strand at 2187.885 kb on - strand at 2187.885 kb on - strand at 2188.134 kb on + strand, within RR42_RS10225 at 2188.134 kb on + strand, within RR42_RS10225 at 2188.167 kb on + strand, within RR42_RS10225 at 2188.167 kb on + strand, within RR42_RS10225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 2,185,388 + RR42_RS10210 0.71 +1.5 2,185,388 + RR42_RS10210 0.71 +0.1 2,185,388 + RR42_RS10210 0.71 -1.5 2,185,389 - RR42_RS10210 0.71 -0.4 2,185,389 - RR42_RS10210 0.71 +0.4 2,185,463 - RR42_RS10210 0.79 +0.8 2,185,507 + RR42_RS10210 0.83 -0.2 2,185,547 + RR42_RS10210 0.87 -0.8 2,185,547 + RR42_RS10210 0.87 +0.0 2,185,654 + -4.1 2,185,841 + -0.5 2,185,858 - +0.2 2,185,858 - -0.6 2,185,884 + -0.7 2,185,887 + -1.3 2,185,888 - +0.7 2,185,888 - +0.2 2,186,045 + -0.0 2,186,046 - -0.2 2,186,046 - -0.4 2,186,268 + RR42_RS10215 0.11 +0.0 2,186,268 + RR42_RS10215 0.11 -0.3 2,186,269 - RR42_RS10215 0.11 -0.8 2,186,448 + RR42_RS10215 0.27 -0.1 2,186,448 + RR42_RS10215 0.27 +0.1 2,186,449 - RR42_RS10215 0.27 +2.4 2,186,450 + RR42_RS10215 0.27 +0.5 2,186,450 + RR42_RS10215 0.27 +0.2 2,186,450 + RR42_RS10215 0.27 -1.3 2,186,451 - RR42_RS10215 0.27 +0.5 2,186,451 - RR42_RS10215 0.27 -0.1 2,186,451 - RR42_RS10215 0.27 -1.7 2,186,640 - RR42_RS10215 0.45 -2.1 2,186,822 + RR42_RS10215 0.61 -0.5 2,186,841 - RR42_RS10215 0.63 -0.7 2,186,876 - RR42_RS10215 0.66 -0.0 2,187,011 + RR42_RS10215 0.78 -0.6 2,187,052 + RR42_RS10215 0.82 -0.7 2,187,052 + RR42_RS10215 0.82 -1.0 2,187,101 + RR42_RS10215 0.86 +0.2 2,187,101 + RR42_RS10215 0.86 -0.2 2,187,102 - RR42_RS10215 0.86 -0.7 2,187,105 - RR42_RS10215 0.86 -0.4 2,187,259 + -0.8 2,187,259 + -1.2 2,187,259 + -1.0 2,187,265 - +0.1 2,187,614 + RR42_RS10220 0.39 -0.4 2,187,615 - RR42_RS10220 0.39 +0.4 2,187,615 - RR42_RS10220 0.39 -0.3 2,187,615 - RR42_RS10220 0.39 -0.5 2,187,618 + RR42_RS10220 0.40 -0.7 2,187,743 + RR42_RS10220 0.66 -0.2 2,187,745 + RR42_RS10220 0.66 -0.7 2,187,764 - RR42_RS10220 0.70 +0.3 2,187,884 + -0.2 2,187,885 - +1.5 2,187,885 - +1.6 2,188,134 + RR42_RS10225 0.21 -0.2 2,188,134 + RR42_RS10225 0.21 +0.1 2,188,167 + RR42_RS10225 0.24 -0.6 2,188,167 + RR42_RS10225 0.24 +0.1
Or see this region's nucleotide sequence