Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS09980

Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.005 mg/ml
2,128,696	+		RR42_RS09970	0.75	+0.5
2,128,697	-		RR42_RS09970	0.75	+0.2
2,128,743	+		RR42_RS09970	0.78	-2.4
2,128,793	+		RR42_RS09970	0.82	+0.8
2,128,794	-		RR42_RS09970	0.82	-0.6
2,128,960	+				-1.6
2,128,960	+				-1.2
2,128,986	-				-0.8
2,129,138	+				-0.4
2,129,138	+				-0.3
2,129,205	-		RR42_RS09975	0.16	+1.1
2,129,329	+		RR42_RS09975	0.42	+0.1
2,129,330	-		RR42_RS09975	0.42	-1.0
2,129,364	-		RR42_RS09975	0.49	+0.5
2,129,373	+		RR42_RS09975	0.51	-0.6
2,129,524	+		RR42_RS09975	0.83	+0.1
2,129,525	-		RR42_RS09975	0.84	+0.9
2,129,525	-		RR42_RS09975	0.84	+0.3
2,129,622	+				+0.2
2,129,623	-				+1.9
2,129,713	+				+0.4
2,129,713	+				-1.1
2,129,713	+				+0.4
2,129,766	+				-0.0
2,129,766	+				+0.7
2,129,924	+				+0.4
2,129,924	+				-0.1
2,129,924	+				+2.1
2,129,925	-				+0.3
2,129,925	-				+0.7
2,129,925	-				-0.1
2,129,974	-				+0.7
2,130,077	+		RR42_RS09980	0.12	+0.7
2,130,077	+		RR42_RS09980	0.12	-0.2
2,130,311	+		RR42_RS09980	0.19	+0.2
2,130,380	-		RR42_RS09980	0.21	-0.6
2,130,414	+		RR42_RS09980	0.22	+0.3
2,130,414	+		RR42_RS09980	0.22	+0.9
2,130,457	-		RR42_RS09980	0.24	-0.3
2,130,464	+		RR42_RS09980	0.24	-0.0
2,130,465	-		RR42_RS09980	0.24	-0.3
2,130,507	+		RR42_RS09980	0.25	-1.5
2,130,507	+		RR42_RS09980	0.25	+0.0
2,130,507	+		RR42_RS09980	0.25	-1.8
2,130,507	+		RR42_RS09980	0.25	+0.1
2,130,507	+		RR42_RS09980	0.25	+1.1
2,130,508	-		RR42_RS09980	0.25	+0.4
2,130,508	-		RR42_RS09980	0.25	+0.6
2,130,508	-		RR42_RS09980	0.25	-1.0
2,130,546	+		RR42_RS09980	0.26	+1.0
2,130,547	-		RR42_RS09980	0.26	-0.8
2,130,682	+		RR42_RS09980	0.31	-0.3
2,130,793	+		RR42_RS09980	0.34	+0.5
2,130,793	+		RR42_RS09980	0.34	+0.2
2,131,107	+		RR42_RS09980	0.43	+0.7
2,131,107	+		RR42_RS09980	0.43	+0.6
2,131,107	+		RR42_RS09980	0.43	+0.0
2,131,107	+		RR42_RS09980	0.43	+0.1
2,131,108	-		RR42_RS09980	0.43	+0.3
2,131,135	+		RR42_RS09980	0.44	+0.8
2,131,161	-		RR42_RS09980	0.45	-1.0
2,131,231	-		RR42_RS09980	0.47	+0.3
2,131,513	+		RR42_RS09980	0.55	-0.9
2,131,514	-		RR42_RS09980	0.55	-1.1
2,131,770	+		RR42_RS09980	0.63	+0.4
2,131,981	-		RR42_RS09980	0.69	-1.2
2,132,104	+		RR42_RS09980	0.73	-0.0
2,132,104	+		RR42_RS09980	0.73	+0.5
2,132,156	-		RR42_RS09980	0.75	-0.1
2,132,156	-		RR42_RS09980	0.75	-0.5
2,132,158	-		RR42_RS09980	0.75	-0.2
2,132,215	-		RR42_RS09980	0.76	-0.5
2,132,282	+		RR42_RS09980	0.78	-0.8
2,132,307	+		RR42_RS09980	0.79	-1.3
2,132,532	+		RR42_RS09980	0.86	-0.3
2,132,532	+		RR42_RS09980	0.86	+0.0
2,132,532	+		RR42_RS09980	0.86	+1.3
2,132,533	-		RR42_RS09980	0.86	-0.5
2,132,547	+		RR42_RS09980	0.86	-0.3
2,132,548	-		RR42_RS09980	0.86	-0.1
2,132,658	+		RR42_RS09980	0.90	+0.8
2,132,658	+		RR42_RS09980	0.90	+0.3
2,132,659	-		RR42_RS09980	0.90	+1.6
2,132,659	-		RR42_RS09980	0.90	+0.6
2,132,668	-				-0.1
2,132,668	-				+0.3
2,132,780	+				-0.1
2,132,796	+				+0.7
2,132,796	+				-1.5
2,132,797	-				+0.6
2,132,797	-				-0.0
2,132,887	-				-0.4
2,132,941	+				+0.1
2,132,954	-				-0.0
2,132,990	+				+2.5
2,133,040	+				+0.0
2,133,041	-				+0.9
2,133,041	-				+0.6
2,133,127	-				+0.7
2,133,127	-				+0.5
2,133,135	-				+0.2
2,133,286	+				-0.2
2,133,287	-				+0.7
2,133,289	+				+0.5
2,133,290	-				-0.2
2,133,290	-				-0.4
2,133,339	+				-0.4
2,133,340	-				-0.8
2,133,340	-				+0.4
2,133,419	-				+1.7
2,133,486	+				+0.5
2,133,486	+				-1.2
2,133,521	+		RR42_RS09985	0.15	+0.1
2,133,522	-		RR42_RS09985	0.16	+1.3
2,133,522	-		RR42_RS09985	0.16	+0.4
2,133,550	+		RR42_RS09985	0.22	+0.9
2,133,550	+		RR42_RS09985	0.22	-0.1
2,133,554	-		RR42_RS09985	0.23	-0.0
2,133,688	+		RR42_RS09985	0.56	+0.2
2,133,689	-		RR42_RS09985	0.56	+0.2
2,133,776	-		RR42_RS09985	0.77	+0.3
2,133,776	-		RR42_RS09985	0.77	-2.3
2,133,795	-		RR42_RS09985	0.82	+0.6
2,133,814	+		RR42_RS09985	0.86	+0.8
2,133,860	+				-1.8
2,133,860	+				-0.5
2,133,860	+				+0.5
2,133,861	-				-0.3
2,133,861	-				+0.4
2,133,861	-				-0.2
2,133,862	+				+0.1
2,133,863	-				+0.2
2,133,863	-				+0.8
2,133,863	-				-0.6
2,133,863	-				-0.4
2,133,895	+				-0.9
2,133,933	-				+1.0
2,133,933	-				-0.7
2,133,933	-				+0.6
2,133,933	-				-2.4