Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09320 and RR42_RS09325 overlap by 4 nucleotides RR42_RS09325 and RR42_RS09330 are separated by 55 nucleotides
RR42_RS09320: RR42_RS09320 - long-chain fatty acid--CoA ligase, at 1,970,561 to 1,972,123
_RS09320
RR42_RS09325: RR42_RS09325 - citrate synthase, at 1,972,120 to 1,972,917
_RS09325
RR42_RS09330: RR42_RS09330 - 3-oxoacyl-ACP reductase, at 1,972,973 to 1,973,773
_RS09330
Position (kb)
1972
1973 Strain fitness (log2 ratio)
-2
-1
0
1 at 1971.146 kb on + strand, within RR42_RS09320 at 1971.147 kb on - strand, within RR42_RS09320 at 1971.147 kb on - strand, within RR42_RS09320 at 1971.311 kb on + strand, within RR42_RS09320 at 1971.311 kb on + strand, within RR42_RS09320 at 1971.311 kb on + strand, within RR42_RS09320 at 1971.312 kb on - strand, within RR42_RS09320 at 1971.374 kb on + strand, within RR42_RS09320 at 1971.449 kb on + strand, within RR42_RS09320 at 1971.728 kb on + strand, within RR42_RS09320 at 1971.734 kb on + strand, within RR42_RS09320 at 1971.735 kb on - strand, within RR42_RS09320 at 1971.735 kb on - strand, within RR42_RS09320 at 1971.735 kb on - strand, within RR42_RS09320 at 1972.174 kb on - strand at 1972.363 kb on + strand, within RR42_RS09325 at 1972.363 kb on + strand, within RR42_RS09325 at 1972.364 kb on - strand, within RR42_RS09325 at 1972.523 kb on - strand, within RR42_RS09325 at 1972.591 kb on + strand, within RR42_RS09325 at 1972.698 kb on + strand, within RR42_RS09325 at 1972.698 kb on + strand, within RR42_RS09325 at 1972.699 kb on - strand, within RR42_RS09325 at 1972.877 kb on - strand at 1973.066 kb on + strand, within RR42_RS09330 at 1973.432 kb on + strand, within RR42_RS09330 at 1973.433 kb on - strand, within RR42_RS09330 at 1973.433 kb on - strand, within RR42_RS09330 at 1973.510 kb on + strand, within RR42_RS09330 at 1973.510 kb on + strand, within RR42_RS09330 at 1973.511 kb on - strand, within RR42_RS09330 at 1973.511 kb on - strand, within RR42_RS09330 at 1973.511 kb on - strand, within RR42_RS09330 at 1973.511 kb on - strand, within RR42_RS09330 at 1973.511 kb on - strand, within RR42_RS09330 at 1973.717 kb on + strand at 1973.717 kb on + strand at 1973.801 kb on - strand at 1973.801 kb on - strand at 1973.801 kb on - strand at 1973.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,971,146 + RR42_RS09320 0.37 -0.6 1,971,147 - RR42_RS09320 0.37 -0.3 1,971,147 - RR42_RS09320 0.37 -0.2 1,971,311 + RR42_RS09320 0.48 +0.0 1,971,311 + RR42_RS09320 0.48 -1.7 1,971,311 + RR42_RS09320 0.48 -0.5 1,971,312 - RR42_RS09320 0.48 -0.7 1,971,374 + RR42_RS09320 0.52 +0.1 1,971,449 + RR42_RS09320 0.57 -0.2 1,971,728 + RR42_RS09320 0.75 -0.3 1,971,734 + RR42_RS09320 0.75 -2.0 1,971,735 - RR42_RS09320 0.75 +0.3 1,971,735 - RR42_RS09320 0.75 -0.2 1,971,735 - RR42_RS09320 0.75 -0.2 1,972,174 - +0.3 1,972,363 + RR42_RS09325 0.30 -0.3 1,972,363 + RR42_RS09325 0.30 -0.2 1,972,364 - RR42_RS09325 0.31 +0.3 1,972,523 - RR42_RS09325 0.51 -0.3 1,972,591 + RR42_RS09325 0.59 +1.7 1,972,698 + RR42_RS09325 0.72 -0.7 1,972,698 + RR42_RS09325 0.72 -0.2 1,972,699 - RR42_RS09325 0.73 -1.0 1,972,877 - -0.3 1,973,066 + RR42_RS09330 0.12 +0.3 1,973,432 + RR42_RS09330 0.57 +0.2 1,973,433 - RR42_RS09330 0.57 +0.9 1,973,433 - RR42_RS09330 0.57 -0.2 1,973,510 + RR42_RS09330 0.67 -0.6 1,973,510 + RR42_RS09330 0.67 -0.7 1,973,511 - RR42_RS09330 0.67 -1.3 1,973,511 - RR42_RS09330 0.67 -0.2 1,973,511 - RR42_RS09330 0.67 -0.6 1,973,511 - RR42_RS09330 0.67 -1.9 1,973,511 - RR42_RS09330 0.67 +0.9 1,973,717 + +0.2 1,973,717 + -1.5 1,973,801 - +0.2 1,973,801 - -0.9 1,973,801 - +0.2 1,973,835 - +0.5
Or see this region's nucleotide sequence