Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08460 and RR42_RS08465 are separated by 61 nucleotides RR42_RS08465 and RR42_RS08470 are separated by 212 nucleotides RR42_RS08470 and RR42_RS08475 are separated by 20 nucleotides RR42_RS08475 and RR42_RS08480 overlap by 8 nucleotides RR42_RS08480 and RR42_RS08485 are separated by 405 nucleotides
RR42_RS08460: RR42_RS08460 - integrase, at 1,782,992 to 1,783,996
_RS08460
RR42_RS08465: RR42_RS08465 - hypothetical protein, at 1,784,058 to 1,784,357
_RS08465
RR42_RS08470: RR42_RS08470 - hypothetical protein, at 1,784,570 to 1,784,776
_RS08470
RR42_RS08475: RR42_RS08475 - hypothetical protein, at 1,784,797 to 1,785,000
_RS08475
RR42_RS08480: RR42_RS08480 - hypothetical protein, at 1,784,993 to 1,785,235
_RS08480
RR42_RS08485: RR42_RS08485 - toprim domain protein, at 1,785,641 to 1,788,346
_RS08485
Position (kb)
1784
1785
1786 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1783.855 kb on + strand, within RR42_RS08460 at 1783.927 kb on + strand at 1783.952 kb on + strand at 1783.955 kb on + strand at 1783.956 kb on - strand at 1783.956 kb on - strand at 1783.956 kb on - strand at 1783.964 kb on - strand at 1784.010 kb on + strand at 1784.010 kb on + strand at 1784.036 kb on + strand at 1784.059 kb on + strand at 1784.059 kb on + strand at 1784.060 kb on - strand at 1784.248 kb on + strand, within RR42_RS08465 at 1784.249 kb on - strand, within RR42_RS08465 at 1784.427 kb on + strand at 1784.427 kb on + strand at 1784.428 kb on - strand at 1784.428 kb on - strand at 1784.518 kb on - strand at 1784.561 kb on + strand at 1784.561 kb on + strand at 1784.562 kb on - strand at 1784.562 kb on - strand at 1784.562 kb on - strand at 1784.570 kb on - strand at 1784.698 kb on - strand, within RR42_RS08470 at 1784.796 kb on - strand at 1784.833 kb on - strand, within RR42_RS08475 at 1785.045 kb on + strand, within RR42_RS08480 at 1785.109 kb on - strand, within RR42_RS08480 at 1785.191 kb on + strand, within RR42_RS08480 at 1785.287 kb on - strand at 1785.374 kb on - strand at 1785.374 kb on - strand at 1785.551 kb on + strand at 1785.569 kb on - strand at 1785.638 kb on + strand at 1785.638 kb on + strand at 1785.652 kb on + strand at 1785.948 kb on + strand, within RR42_RS08485
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,783,855 + RR42_RS08460 0.86 +0.6 1,783,927 + -0.7 1,783,952 + -0.1 1,783,955 + -0.6 1,783,956 - +0.3 1,783,956 - -1.0 1,783,956 - +0.2 1,783,964 - -0.1 1,784,010 + +0.5 1,784,010 + +0.1 1,784,036 + -0.4 1,784,059 + +1.8 1,784,059 + -0.3 1,784,060 - -0.5 1,784,248 + RR42_RS08465 0.63 +0.5 1,784,249 - RR42_RS08465 0.64 +0.5 1,784,427 + -0.2 1,784,427 + +2.0 1,784,428 - +0.9 1,784,428 - -0.3 1,784,518 - -0.3 1,784,561 + +1.3 1,784,561 + +0.9 1,784,562 - +0.5 1,784,562 - -0.1 1,784,562 - -0.4 1,784,570 - -0.3 1,784,698 - RR42_RS08470 0.62 -1.2 1,784,796 - -1.0 1,784,833 - RR42_RS08475 0.18 -0.9 1,785,045 + RR42_RS08480 0.21 +0.1 1,785,109 - RR42_RS08480 0.48 -0.3 1,785,191 + RR42_RS08480 0.81 -0.6 1,785,287 - +0.6 1,785,374 - -0.1 1,785,374 - -0.6 1,785,551 + +0.4 1,785,569 - -0.8 1,785,638 + -2.0 1,785,638 + -0.5 1,785,652 + -0.6 1,785,948 + RR42_RS08485 0.11 +0.3
Or see this region's nucleotide sequence