Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08450 and RR42_RS08455 overlap by 13 nucleotides RR42_RS08455 and RR42_RS08460 are separated by 555 nucleotides RR42_RS08460 and RR42_RS08465 are separated by 61 nucleotides RR42_RS08465 and RR42_RS08470 are separated by 212 nucleotides RR42_RS08470 and RR42_RS08475 are separated by 20 nucleotides
RR42_RS08450: RR42_RS08450 - hypothetical protein, at 1,781,820 to 1,782,086
_RS08450
RR42_RS08455: RR42_RS08455 - hypothetical protein, at 1,782,074 to 1,782,436
_RS08455
RR42_RS08460: RR42_RS08460 - integrase, at 1,782,992 to 1,783,996
_RS08460
RR42_RS08465: RR42_RS08465 - hypothetical protein, at 1,784,058 to 1,784,357
_RS08465
RR42_RS08470: RR42_RS08470 - hypothetical protein, at 1,784,570 to 1,784,776
_RS08470
RR42_RS08475: RR42_RS08475 - hypothetical protein, at 1,784,797 to 1,785,000
_RS08475
RR42_RS08480: RR42_RS08480 - hypothetical protein, at 1,784,993 to 1,785,235
_RS08480
Position (kb)
1782
1783
1784 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1782.070 kb on + strand at 1782.070 kb on + strand at 1782.070 kb on + strand at 1782.071 kb on - strand at 1782.108 kb on + strand at 1782.108 kb on + strand at 1782.201 kb on + strand, within RR42_RS08455 at 1782.201 kb on + strand, within RR42_RS08455 at 1782.400 kb on + strand at 1782.401 kb on - strand at 1782.401 kb on - strand at 1782.401 kb on - strand at 1782.401 kb on - strand at 1782.401 kb on - strand at 1782.402 kb on + strand at 1782.403 kb on - strand at 1782.403 kb on - strand at 1782.403 kb on - strand at 1782.404 kb on + strand at 1782.404 kb on + strand at 1782.404 kb on + strand at 1782.404 kb on + strand at 1782.404 kb on + strand at 1782.404 kb on + strand at 1782.404 kb on + strand at 1782.404 kb on + strand at 1782.404 kb on + strand at 1782.404 kb on + strand at 1782.404 kb on + strand at 1782.405 kb on - strand at 1782.405 kb on - strand at 1782.405 kb on - strand at 1782.405 kb on - strand at 1782.405 kb on - strand at 1782.405 kb on - strand at 1782.405 kb on - strand at 1782.405 kb on - strand at 1782.405 kb on - strand at 1782.405 kb on - strand at 1782.405 kb on - strand at 1782.408 kb on + strand at 1782.408 kb on + strand at 1782.408 kb on + strand at 1782.408 kb on + strand at 1782.409 kb on - strand at 1782.409 kb on - strand at 1782.457 kb on + strand at 1782.484 kb on + strand at 1782.484 kb on + strand at 1782.485 kb on - strand at 1782.499 kb on + strand at 1782.499 kb on + strand at 1782.499 kb on + strand at 1782.500 kb on - strand at 1782.500 kb on - strand at 1782.581 kb on + strand at 1782.582 kb on - strand at 1782.582 kb on - strand at 1782.583 kb on + strand at 1782.584 kb on - strand at 1782.584 kb on - strand at 1782.584 kb on - strand at 1782.672 kb on + strand at 1782.854 kb on + strand at 1782.855 kb on - strand at 1782.864 kb on + strand at 1782.871 kb on - strand at 1782.876 kb on - strand at 1782.876 kb on - strand at 1782.909 kb on + strand at 1783.101 kb on + strand, within RR42_RS08460 at 1783.156 kb on + strand, within RR42_RS08460 at 1783.157 kb on - strand, within RR42_RS08460 at 1783.230 kb on - strand, within RR42_RS08460 at 1783.339 kb on - strand, within RR42_RS08460 at 1783.529 kb on + strand, within RR42_RS08460 at 1783.530 kb on - strand, within RR42_RS08460 at 1783.554 kb on + strand, within RR42_RS08460 at 1783.554 kb on + strand, within RR42_RS08460 at 1783.558 kb on + strand, within RR42_RS08460 at 1783.559 kb on - strand, within RR42_RS08460 at 1783.559 kb on - strand, within RR42_RS08460 at 1783.855 kb on + strand, within RR42_RS08460 at 1783.927 kb on + strand at 1783.952 kb on + strand at 1783.955 kb on + strand at 1783.956 kb on - strand at 1783.956 kb on - strand at 1783.956 kb on - strand at 1783.964 kb on - strand at 1784.010 kb on + strand at 1784.010 kb on + strand at 1784.036 kb on + strand at 1784.059 kb on + strand at 1784.059 kb on + strand at 1784.060 kb on - strand at 1784.248 kb on + strand, within RR42_RS08465 at 1784.249 kb on - strand, within RR42_RS08465 at 1784.427 kb on + strand at 1784.427 kb on + strand at 1784.428 kb on - strand at 1784.428 kb on - strand at 1784.518 kb on - strand at 1784.561 kb on + strand at 1784.561 kb on + strand at 1784.562 kb on - strand at 1784.562 kb on - strand at 1784.562 kb on - strand at 1784.570 kb on - strand at 1784.698 kb on - strand, within RR42_RS08470 at 1784.796 kb on - strand at 1784.833 kb on - strand, within RR42_RS08475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,782,070 + +0.0 1,782,070 + -0.8 1,782,070 + +0.1 1,782,071 - +0.1 1,782,108 + +0.1 1,782,108 + +0.3 1,782,201 + RR42_RS08455 0.35 -0.1 1,782,201 + RR42_RS08455 0.35 +0.0 1,782,400 + -0.8 1,782,401 - -1.7 1,782,401 - -1.2 1,782,401 - +0.3 1,782,401 - +0.8 1,782,401 - +0.1 1,782,402 + -0.3 1,782,403 - -0.2 1,782,403 - +0.5 1,782,403 - +1.0 1,782,404 + +1.6 1,782,404 + +0.5 1,782,404 + +0.4 1,782,404 + +0.1 1,782,404 + +0.2 1,782,404 + +0.8 1,782,404 + -0.9 1,782,404 + +0.3 1,782,404 + -0.5 1,782,404 + +0.9 1,782,404 + +0.4 1,782,405 - -0.1 1,782,405 - -1.9 1,782,405 - +0.0 1,782,405 - +0.3 1,782,405 - +0.5 1,782,405 - -0.1 1,782,405 - -1.4 1,782,405 - -0.4 1,782,405 - +1.1 1,782,405 - +0.1 1,782,405 - +1.1 1,782,408 + -0.7 1,782,408 + +0.3 1,782,408 + -0.1 1,782,408 + +0.3 1,782,409 - -0.3 1,782,409 - +0.0 1,782,457 + -0.3 1,782,484 + -0.4 1,782,484 + +0.6 1,782,485 - -0.3 1,782,499 + -0.3 1,782,499 + +0.2 1,782,499 + -0.9 1,782,500 - -0.5 1,782,500 - -0.6 1,782,581 + +0.3 1,782,582 - +0.5 1,782,582 - -0.1 1,782,583 + -0.6 1,782,584 - +0.0 1,782,584 - -0.0 1,782,584 - -0.4 1,782,672 + -0.4 1,782,854 + +0.1 1,782,855 - -0.8 1,782,864 + +1.1 1,782,871 - -0.6 1,782,876 - +1.4 1,782,876 - +0.3 1,782,909 + +0.3 1,783,101 + RR42_RS08460 0.11 +1.1 1,783,156 + RR42_RS08460 0.16 -0.1 1,783,157 - RR42_RS08460 0.16 +0.3 1,783,230 - RR42_RS08460 0.24 -0.4 1,783,339 - RR42_RS08460 0.35 +0.2 1,783,529 + RR42_RS08460 0.53 +1.1 1,783,530 - RR42_RS08460 0.54 +0.6 1,783,554 + RR42_RS08460 0.56 +0.3 1,783,554 + RR42_RS08460 0.56 -0.3 1,783,558 + RR42_RS08460 0.56 +0.3 1,783,559 - RR42_RS08460 0.56 +1.0 1,783,559 - RR42_RS08460 0.56 +0.2 1,783,855 + RR42_RS08460 0.86 +0.6 1,783,927 + -0.7 1,783,952 + -0.1 1,783,955 + -0.6 1,783,956 - +0.3 1,783,956 - -1.0 1,783,956 - +0.2 1,783,964 - -0.1 1,784,010 + +0.5 1,784,010 + +0.1 1,784,036 + -0.4 1,784,059 + +1.8 1,784,059 + -0.3 1,784,060 - -0.5 1,784,248 + RR42_RS08465 0.63 +0.5 1,784,249 - RR42_RS08465 0.64 +0.5 1,784,427 + -0.2 1,784,427 + +2.0 1,784,428 - +0.9 1,784,428 - -0.3 1,784,518 - -0.3 1,784,561 + +1.3 1,784,561 + +0.9 1,784,562 - +0.5 1,784,562 - -0.1 1,784,562 - -0.4 1,784,570 - -0.3 1,784,698 - RR42_RS08470 0.62 -1.2 1,784,796 - -1.0 1,784,833 - RR42_RS08475 0.18 -0.9
Or see this region's nucleotide sequence