Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08035 and RR42_RS08040 are separated by 113 nucleotides RR42_RS08040 and RR42_RS08045 are separated by 26 nucleotides RR42_RS08045 and RR42_RS08050 are separated by 307 nucleotides RR42_RS08050 and RR42_RS08055 are separated by 148 nucleotides
RR42_RS08035: RR42_RS08035 - DNA methyltransferase, at 1,722,173 to 1,723,957
_RS08035
RR42_RS08040: RR42_RS08040 - hypothetical protein, at 1,724,071 to 1,724,271
_RS08040
RR42_RS08045: RR42_RS08045 - hypothetical protein, at 1,724,298 to 1,724,849
_RS08045
RR42_RS08050: RR42_RS08050 - hypothetical protein, at 1,725,157 to 1,725,387
_RS08050
RR42_RS08055: RR42_RS08055 - hypothetical protein, at 1,725,536 to 1,726,153
_RS08055
Position (kb) 1724
1725 Strain fitness (log2 ratio) -1
0
1 at 1723.308 kb on + strand, within RR42_RS08035 at 1723.308 kb on + strand, within RR42_RS08035 at 1723.309 kb on - strand, within RR42_RS08035 at 1723.747 kb on - strand, within RR42_RS08035 at 1724.008 kb on + strand at 1724.034 kb on - strand at 1724.050 kb on + strand at 1724.070 kb on + strand at 1724.070 kb on + strand at 1724.138 kb on - strand, within RR42_RS08040 at 1724.167 kb on + strand, within RR42_RS08040 at 1724.170 kb on + strand, within RR42_RS08040 at 1724.227 kb on + strand, within RR42_RS08040 at 1724.228 kb on - strand, within RR42_RS08040 at 1724.267 kb on - strand at 1724.280 kb on + strand at 1724.280 kb on + strand at 1724.371 kb on - strand, within RR42_RS08045 at 1724.572 kb on + strand, within RR42_RS08045 at 1724.572 kb on + strand, within RR42_RS08045 at 1724.572 kb on + strand, within RR42_RS08045 at 1724.675 kb on - strand, within RR42_RS08045 at 1724.722 kb on + strand, within RR42_RS08045 at 1724.722 kb on + strand, within RR42_RS08045 at 1725.072 kb on + strand at 1725.251 kb on + strand, within RR42_RS08050 at 1725.403 kb on - strand at 1725.573 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,723,308 + RR42_RS08035 0.64 -0.0 1,723,308 + RR42_RS08035 0.64 +1.2 1,723,309 - RR42_RS08035 0.64 +0.6 1,723,747 - RR42_RS08035 0.88 -1.5 1,724,008 + -0.6 1,724,034 - -0.8 1,724,050 + -0.5 1,724,070 + -0.4 1,724,070 + +0.6 1,724,138 - RR42_RS08040 0.33 +0.2 1,724,167 + RR42_RS08040 0.48 -1.0 1,724,170 + RR42_RS08040 0.49 -1.2 1,724,227 + RR42_RS08040 0.78 +1.6 1,724,228 - RR42_RS08040 0.78 -0.6 1,724,267 - -0.2 1,724,280 + +0.1 1,724,280 + +0.4 1,724,371 - RR42_RS08045 0.13 +0.4 1,724,572 + RR42_RS08045 0.50 -0.3 1,724,572 + RR42_RS08045 0.50 -0.3 1,724,572 + RR42_RS08045 0.50 +0.7 1,724,675 - RR42_RS08045 0.68 +0.1 1,724,722 + RR42_RS08045 0.77 -1.4 1,724,722 + RR42_RS08045 0.77 -0.1 1,725,072 + +0.3 1,725,251 + RR42_RS08050 0.41 -1.7 1,725,403 - +0.2 1,725,573 + -1.1
Or see this region's nucleotide sequence