Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07880 and RR42_RS07885 are separated by 13 nucleotides RR42_RS07885 and RR42_RS07890 are separated by 255 nucleotides RR42_RS07890 and RR42_RS07895 are separated by 110 nucleotides
RR42_RS07880: RR42_RS07880 - histidine kinase, at 1,691,235 to 1,692,584
_RS07880
RR42_RS07885: RR42_RS07885 - chemotaxis protein CheY, at 1,692,598 to 1,693,320
_RS07885
RR42_RS07890: RR42_RS07890 - hypothetical protein, at 1,693,576 to 1,693,917
_RS07890
RR42_RS07895: RR42_RS07895 - gluconolactonase, at 1,694,028 to 1,694,924
_RS07895
Position (kb)
1692
1693
1694 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1691.628 kb on + strand, within RR42_RS07880 at 1691.628 kb on + strand, within RR42_RS07880 at 1691.628 kb on + strand, within RR42_RS07880 at 1691.628 kb on + strand, within RR42_RS07880 at 1691.628 kb on + strand, within RR42_RS07880 at 1691.629 kb on - strand, within RR42_RS07880 at 1691.629 kb on - strand, within RR42_RS07880 at 1691.629 kb on - strand, within RR42_RS07880 at 1691.647 kb on + strand, within RR42_RS07880 at 1691.648 kb on - strand, within RR42_RS07880 at 1691.719 kb on + strand, within RR42_RS07880 at 1691.719 kb on + strand, within RR42_RS07880 at 1691.720 kb on - strand, within RR42_RS07880 at 1692.073 kb on - strand, within RR42_RS07880 at 1692.149 kb on - strand, within RR42_RS07880 at 1692.180 kb on + strand, within RR42_RS07880 at 1692.181 kb on - strand, within RR42_RS07880 at 1692.181 kb on - strand, within RR42_RS07880 at 1692.317 kb on - strand, within RR42_RS07880 at 1692.383 kb on - strand, within RR42_RS07880 at 1692.383 kb on - strand, within RR42_RS07880 at 1692.383 kb on - strand, within RR42_RS07880 at 1692.383 kb on - strand, within RR42_RS07880 at 1692.470 kb on - strand at 1692.485 kb on + strand at 1692.599 kb on + strand at 1692.599 kb on + strand at 1692.626 kb on + strand at 1692.626 kb on + strand at 1692.626 kb on + strand at 1692.627 kb on - strand at 1692.627 kb on - strand at 1692.627 kb on - strand at 1692.735 kb on - strand, within RR42_RS07885 at 1693.162 kb on + strand, within RR42_RS07885 at 1693.163 kb on - strand, within RR42_RS07885 at 1693.163 kb on - strand, within RR42_RS07885 at 1693.219 kb on + strand, within RR42_RS07885 at 1693.219 kb on + strand, within RR42_RS07885 at 1693.219 kb on + strand, within RR42_RS07885 at 1693.220 kb on - strand, within RR42_RS07885 at 1693.220 kb on - strand, within RR42_RS07885 at 1693.220 kb on - strand, within RR42_RS07885 at 1693.225 kb on + strand, within RR42_RS07885 at 1693.225 kb on + strand, within RR42_RS07885 at 1693.226 kb on - strand, within RR42_RS07885 at 1693.326 kb on + strand at 1693.332 kb on - strand at 1693.372 kb on + strand at 1693.373 kb on - strand at 1693.373 kb on - strand at 1693.376 kb on + strand at 1693.376 kb on + strand at 1693.376 kb on + strand at 1693.377 kb on - strand at 1693.377 kb on - strand at 1693.377 kb on - strand at 1693.391 kb on + strand at 1693.419 kb on - strand at 1693.479 kb on + strand at 1693.479 kb on + strand at 1693.480 kb on - strand at 1693.511 kb on + strand at 1693.571 kb on + strand at 1693.572 kb on - strand at 1693.572 kb on - strand at 1693.650 kb on + strand, within RR42_RS07890 at 1693.651 kb on - strand, within RR42_RS07890 at 1693.750 kb on + strand, within RR42_RS07890 at 1693.883 kb on - strand at 1693.903 kb on + strand at 1693.903 kb on + strand at 1693.903 kb on + strand at 1693.904 kb on - strand at 1693.904 kb on - strand at 1693.904 kb on - strand at 1693.904 kb on - strand at 1693.915 kb on + strand at 1693.916 kb on - strand at 1693.916 kb on - strand at 1694.072 kb on + strand at 1694.073 kb on - strand at 1694.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,691,628 + RR42_RS07880 0.29 -0.4 1,691,628 + RR42_RS07880 0.29 -0.9 1,691,628 + RR42_RS07880 0.29 -0.4 1,691,628 + RR42_RS07880 0.29 -0.3 1,691,628 + RR42_RS07880 0.29 -1.8 1,691,629 - RR42_RS07880 0.29 -1.0 1,691,629 - RR42_RS07880 0.29 -0.9 1,691,629 - RR42_RS07880 0.29 -0.2 1,691,647 + RR42_RS07880 0.31 -1.3 1,691,648 - RR42_RS07880 0.31 -1.3 1,691,719 + RR42_RS07880 0.36 -0.1 1,691,719 + RR42_RS07880 0.36 -1.0 1,691,720 - RR42_RS07880 0.36 -0.9 1,692,073 - RR42_RS07880 0.62 +1.1 1,692,149 - RR42_RS07880 0.68 -1.2 1,692,180 + RR42_RS07880 0.70 -0.4 1,692,181 - RR42_RS07880 0.70 +0.4 1,692,181 - RR42_RS07880 0.70 -0.9 1,692,317 - RR42_RS07880 0.80 -0.2 1,692,383 - RR42_RS07880 0.85 -1.2 1,692,383 - RR42_RS07880 0.85 -0.8 1,692,383 - RR42_RS07880 0.85 -2.0 1,692,383 - RR42_RS07880 0.85 -0.5 1,692,470 - -2.2 1,692,485 + -1.3 1,692,599 + -1.6 1,692,599 + -2.8 1,692,626 + -0.7 1,692,626 + -1.0 1,692,626 + -1.3 1,692,627 - -1.2 1,692,627 - -0.3 1,692,627 - -1.1 1,692,735 - RR42_RS07885 0.19 -1.8 1,693,162 + RR42_RS07885 0.78 -1.7 1,693,163 - RR42_RS07885 0.78 -1.2 1,693,163 - RR42_RS07885 0.78 -2.0 1,693,219 + RR42_RS07885 0.86 +2.1 1,693,219 + RR42_RS07885 0.86 -0.5 1,693,219 + RR42_RS07885 0.86 -0.7 1,693,220 - RR42_RS07885 0.86 -1.6 1,693,220 - RR42_RS07885 0.86 +0.3 1,693,220 - RR42_RS07885 0.86 -2.4 1,693,225 + RR42_RS07885 0.87 -0.2 1,693,225 + RR42_RS07885 0.87 -0.9 1,693,226 - RR42_RS07885 0.87 -3.7 1,693,326 + -2.4 1,693,332 - +0.6 1,693,372 + -1.3 1,693,373 - +0.3 1,693,373 - -0.2 1,693,376 + -0.7 1,693,376 + -2.4 1,693,376 + -2.7 1,693,377 - +0.2 1,693,377 - +0.9 1,693,377 - +0.5 1,693,391 + -2.3 1,693,419 - +1.5 1,693,479 + -0.2 1,693,479 + +0.3 1,693,480 - +0.9 1,693,511 + -1.7 1,693,571 + -2.3 1,693,572 - +1.2 1,693,572 - +1.5 1,693,650 + RR42_RS07890 0.22 +1.6 1,693,651 - RR42_RS07890 0.22 +0.8 1,693,750 + RR42_RS07890 0.51 +1.1 1,693,883 - +0.9 1,693,903 + +1.6 1,693,903 + +0.7 1,693,903 + +1.4 1,693,904 - +1.0 1,693,904 - +1.1 1,693,904 - +0.1 1,693,904 - +0.7 1,693,915 + +3.1 1,693,916 - +0.5 1,693,916 - +0.5 1,694,072 + +0.7 1,694,073 - -0.1 1,694,073 - +0.6
Or see this region's nucleotide sequence