Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07580 and RR42_RS07585 are separated by 105 nucleotides RR42_RS07585 and RR42_RS07590 are separated by 16 nucleotides RR42_RS07590 and RR42_RS07595 overlap by 11 nucleotides RR42_RS07595 and RR42_RS07600 are separated by 13 nucleotides
RR42_RS07580: RR42_RS07580 - helicase, at 1,622,488 to 1,624,692
_RS07580
RR42_RS07585: RR42_RS07585 - competence protein ComL, at 1,624,798 to 1,625,628
_RS07585
RR42_RS07590: RR42_RS07590 - pseudouridine synthase, at 1,625,645 to 1,626,862
_RS07590
RR42_RS07595: RR42_RS07595 - hypothetical protein, at 1,626,852 to 1,627,655
_RS07595
RR42_RS07600: RR42_RS07600 - membrane protein, at 1,627,669 to 1,627,929
_RS07600
Position (kb)
1625
1626
1627 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1624.841 kb on + strand at 1624.850 kb on + strand at 1624.850 kb on + strand at 1624.851 kb on - strand at 1624.851 kb on - strand at 1625.667 kb on - strand at 1625.785 kb on - strand, within RR42_RS07590 at 1626.083 kb on + strand, within RR42_RS07590 at 1626.083 kb on + strand, within RR42_RS07590 at 1626.083 kb on + strand, within RR42_RS07590 at 1626.084 kb on - strand, within RR42_RS07590 at 1626.169 kb on - strand, within RR42_RS07590 at 1626.332 kb on + strand, within RR42_RS07590 at 1626.333 kb on - strand, within RR42_RS07590 at 1626.466 kb on + strand, within RR42_RS07590 at 1626.596 kb on + strand, within RR42_RS07590 at 1626.597 kb on - strand, within RR42_RS07590 at 1626.724 kb on - strand, within RR42_RS07590 at 1626.724 kb on - strand, within RR42_RS07590 at 1626.724 kb on - strand, within RR42_RS07590 at 1626.845 kb on + strand at 1626.875 kb on - strand at 1626.875 kb on - strand at 1626.875 kb on - strand at 1626.952 kb on - strand, within RR42_RS07595 at 1627.488 kb on + strand, within RR42_RS07595 at 1627.488 kb on + strand, within RR42_RS07595 at 1627.488 kb on + strand, within RR42_RS07595 at 1627.489 kb on - strand, within RR42_RS07595 at 1627.499 kb on + strand, within RR42_RS07595 at 1627.500 kb on - strand, within RR42_RS07595 at 1627.500 kb on - strand, within RR42_RS07595 at 1627.503 kb on + strand, within RR42_RS07595 at 1627.503 kb on + strand, within RR42_RS07595 at 1627.503 kb on + strand, within RR42_RS07595 at 1627.503 kb on + strand, within RR42_RS07595 at 1627.503 kb on + strand, within RR42_RS07595 at 1627.504 kb on - strand, within RR42_RS07595 at 1627.504 kb on - strand, within RR42_RS07595 at 1627.504 kb on - strand, within RR42_RS07595 at 1627.504 kb on - strand, within RR42_RS07595 at 1627.504 kb on - strand, within RR42_RS07595 at 1627.593 kb on + strand at 1627.593 kb on + strand at 1627.594 kb on - strand at 1627.600 kb on - strand at 1627.805 kb on + strand, within RR42_RS07600 at 1627.805 kb on + strand, within RR42_RS07600 at 1627.806 kb on - strand, within RR42_RS07600 at 1627.806 kb on - strand, within RR42_RS07600 at 1627.806 kb on - strand, within RR42_RS07600 at 1627.806 kb on - strand, within RR42_RS07600 at 1627.806 kb on - strand, within RR42_RS07600 at 1627.835 kb on + strand, within RR42_RS07600 at 1627.836 kb on - strand, within RR42_RS07600 at 1627.836 kb on - strand, within RR42_RS07600 at 1627.836 kb on - strand, within RR42_RS07600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,624,841 + -1.7 1,624,850 + -1.3 1,624,850 + -2.7 1,624,851 - -0.9 1,624,851 - -0.6 1,625,667 - +0.8 1,625,785 - RR42_RS07590 0.11 +0.4 1,626,083 + RR42_RS07590 0.36 +2.6 1,626,083 + RR42_RS07590 0.36 +2.0 1,626,083 + RR42_RS07590 0.36 +1.8 1,626,084 - RR42_RS07590 0.36 +0.4 1,626,169 - RR42_RS07590 0.43 +2.0 1,626,332 + RR42_RS07590 0.56 +2.6 1,626,333 - RR42_RS07590 0.56 +1.6 1,626,466 + RR42_RS07590 0.67 +3.1 1,626,596 + RR42_RS07590 0.78 +4.3 1,626,597 - RR42_RS07590 0.78 +2.1 1,626,724 - RR42_RS07590 0.89 +2.6 1,626,724 - RR42_RS07590 0.89 +2.0 1,626,724 - RR42_RS07590 0.89 +3.0 1,626,845 + +2.2 1,626,875 - -0.0 1,626,875 - -1.3 1,626,875 - +0.4 1,626,952 - RR42_RS07595 0.12 +0.5 1,627,488 + RR42_RS07595 0.79 -0.0 1,627,488 + RR42_RS07595 0.79 +0.2 1,627,488 + RR42_RS07595 0.79 +0.6 1,627,489 - RR42_RS07595 0.79 -0.2 1,627,499 + RR42_RS07595 0.80 +1.6 1,627,500 - RR42_RS07595 0.81 +0.6 1,627,500 - RR42_RS07595 0.81 +0.2 1,627,503 + RR42_RS07595 0.81 +0.4 1,627,503 + RR42_RS07595 0.81 +0.5 1,627,503 + RR42_RS07595 0.81 +0.3 1,627,503 + RR42_RS07595 0.81 -0.2 1,627,503 + RR42_RS07595 0.81 -0.2 1,627,504 - RR42_RS07595 0.81 +0.6 1,627,504 - RR42_RS07595 0.81 -0.1 1,627,504 - RR42_RS07595 0.81 +0.6 1,627,504 - RR42_RS07595 0.81 +1.3 1,627,504 - RR42_RS07595 0.81 +0.4 1,627,593 + -0.3 1,627,593 + +0.2 1,627,594 - -0.1 1,627,600 - +0.2 1,627,805 + RR42_RS07600 0.52 -0.6 1,627,805 + RR42_RS07600 0.52 -0.5 1,627,806 - RR42_RS07600 0.52 -1.8 1,627,806 - RR42_RS07600 0.52 -0.2 1,627,806 - RR42_RS07600 0.52 -0.9 1,627,806 - RR42_RS07600 0.52 -0.1 1,627,806 - RR42_RS07600 0.52 -0.7 1,627,835 + RR42_RS07600 0.64 +0.4 1,627,836 - RR42_RS07600 0.64 -0.9 1,627,836 - RR42_RS07600 0.64 -1.6 1,627,836 - RR42_RS07600 0.64 -1.5
Or see this region's nucleotide sequence