Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07580 and RR42_RS07585 are separated by 105 nucleotides RR42_RS07585 and RR42_RS07590 are separated by 16 nucleotides
RR42_RS07580: RR42_RS07580 - helicase, at 1,622,488 to 1,624,692
_RS07580
RR42_RS07585: RR42_RS07585 - competence protein ComL, at 1,624,798 to 1,625,628
_RS07585
RR42_RS07590: RR42_RS07590 - pseudouridine synthase, at 1,625,645 to 1,626,862
_RS07590
Position (kb) 1624
1625
1626 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1623.910 kb on - strand, within RR42_RS07580 at 1623.910 kb on - strand, within RR42_RS07580 at 1623.910 kb on - strand, within RR42_RS07580 at 1623.914 kb on + strand, within RR42_RS07580 at 1623.915 kb on - strand, within RR42_RS07580 at 1623.940 kb on + strand, within RR42_RS07580 at 1623.940 kb on + strand, within RR42_RS07580 at 1623.940 kb on + strand, within RR42_RS07580 at 1623.941 kb on - strand, within RR42_RS07580 at 1623.941 kb on - strand, within RR42_RS07580 at 1623.941 kb on - strand, within RR42_RS07580 at 1624.001 kb on - strand, within RR42_RS07580 at 1624.021 kb on + strand, within RR42_RS07580 at 1624.171 kb on + strand, within RR42_RS07580 at 1624.171 kb on + strand, within RR42_RS07580 at 1624.172 kb on - strand, within RR42_RS07580 at 1624.172 kb on - strand, within RR42_RS07580 at 1624.172 kb on - strand, within RR42_RS07580 at 1624.339 kb on - strand, within RR42_RS07580 at 1624.527 kb on + strand at 1624.550 kb on - strand at 1624.841 kb on + strand at 1624.850 kb on + strand at 1624.850 kb on + strand at 1624.851 kb on - strand at 1624.851 kb on - strand at 1625.667 kb on - strand at 1625.785 kb on - strand, within RR42_RS07590 at 1626.083 kb on + strand, within RR42_RS07590 at 1626.083 kb on + strand, within RR42_RS07590 at 1626.083 kb on + strand, within RR42_RS07590 at 1626.084 kb on - strand, within RR42_RS07590 at 1626.169 kb on - strand, within RR42_RS07590 at 1626.332 kb on + strand, within RR42_RS07590 at 1626.333 kb on - strand, within RR42_RS07590 at 1626.466 kb on + strand, within RR42_RS07590 at 1626.596 kb on + strand, within RR42_RS07590 at 1626.597 kb on - strand, within RR42_RS07590
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,623,910 - RR42_RS07580 0.64 +1.7 1,623,910 - RR42_RS07580 0.64 -0.2 1,623,910 - RR42_RS07580 0.64 +0.9 1,623,914 + RR42_RS07580 0.65 +0.8 1,623,915 - RR42_RS07580 0.65 -0.6 1,623,940 + RR42_RS07580 0.66 -0.6 1,623,940 + RR42_RS07580 0.66 +0.6 1,623,940 + RR42_RS07580 0.66 -0.1 1,623,941 - RR42_RS07580 0.66 -1.6 1,623,941 - RR42_RS07580 0.66 +0.7 1,623,941 - RR42_RS07580 0.66 -0.8 1,624,001 - RR42_RS07580 0.69 +0.7 1,624,021 + RR42_RS07580 0.70 +0.4 1,624,171 + RR42_RS07580 0.76 +0.1 1,624,171 + RR42_RS07580 0.76 +0.8 1,624,172 - RR42_RS07580 0.76 -0.2 1,624,172 - RR42_RS07580 0.76 +0.5 1,624,172 - RR42_RS07580 0.76 -0.6 1,624,339 - RR42_RS07580 0.84 +0.3 1,624,527 + -0.4 1,624,550 - +0.3 1,624,841 + -1.7 1,624,850 + -1.3 1,624,850 + -2.7 1,624,851 - -0.9 1,624,851 - -0.6 1,625,667 - +0.8 1,625,785 - RR42_RS07590 0.11 +0.4 1,626,083 + RR42_RS07590 0.36 +2.6 1,626,083 + RR42_RS07590 0.36 +2.0 1,626,083 + RR42_RS07590 0.36 +1.8 1,626,084 - RR42_RS07590 0.36 +0.4 1,626,169 - RR42_RS07590 0.43 +2.0 1,626,332 + RR42_RS07590 0.56 +2.6 1,626,333 - RR42_RS07590 0.56 +1.6 1,626,466 + RR42_RS07590 0.67 +3.1 1,626,596 + RR42_RS07590 0.78 +4.3 1,626,597 - RR42_RS07590 0.78 +2.1
Or see this region's nucleotide sequence