Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS07255

Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS07240 and RR42_RS07245 are separated by 188 nucleotidesRR42_RS07245 and RR42_RS07250 are separated by 20 nucleotidesRR42_RS07250 and RR42_RS07255 are separated by 75 nucleotidesRR42_RS07255 and RR42_RS07260 are separated by 97 nucleotidesRR42_RS07260 and RR42_RS07265 are separated by 207 nucleotides RR42_RS07240: RR42_RS07240 - hypothetical protein, at 1,543,788 to 1,544,042 _RS07240 RR42_RS07245: RR42_RS07245 - hypothetical protein, at 1,544,231 to 1,544,521 _RS07245 RR42_RS07250: RR42_RS07250 - hypothetical protein, at 1,544,542 to 1,544,784 _RS07250 RR42_RS07255: RR42_RS07255 - hypothetical protein, at 1,544,860 to 1,545,111 _RS07255 RR42_RS07260: RR42_RS07260 - hypothetical protein, at 1,545,209 to 1,545,577 _RS07260 RR42_RS07265: RR42_RS07265 - hypothetical protein, at 1,545,785 to 1,546,039 _RS07265 Position (kb) 1544 1545 1546Strain fitness (log2 ratio) -1 0 1 2 3at 1543.943 kb on + strand, within RR42_RS07240at 1543.944 kb on - strand, within RR42_RS07240at 1544.041 kb on - strandat 1544.103 kb on - strandat 1544.214 kb on + strandat 1544.214 kb on + strandat 1544.219 kb on + strandat 1544.225 kb on + strandat 1544.226 kb on - strandat 1544.381 kb on - strand, within RR42_RS07245at 1544.420 kb on - strand, within RR42_RS07245at 1544.536 kb on + strandat 1544.783 kb on - strandat 1544.834 kb on + strandat 1544.834 kb on + strandat 1544.834 kb on + strandat 1544.834 kb on + strandat 1544.834 kb on + strandat 1544.835 kb on - strandat 1544.835 kb on - strandat 1544.894 kb on - strand, within RR42_RS07255at 1544.894 kb on - strand, within RR42_RS07255at 1545.097 kb on + strandat 1545.134 kb on - strandat 1545.175 kb on + strandat 1545.175 kb on + strandat 1545.175 kb on + strandat 1545.175 kb on + strandat 1545.176 kb on - strandat 1545.176 kb on - strandat 1545.176 kb on - strandat 1545.407 kb on + strand, within RR42_RS07260at 1545.407 kb on + strand, within RR42_RS07260at 1545.407 kb on + strand, within RR42_RS07260at 1545.407 kb on + strand, within RR42_RS07260at 1545.407 kb on + strand, within RR42_RS07260at 1545.408 kb on - strand, within RR42_RS07260at 1545.408 kb on - strand, within RR42_RS07260at 1545.420 kb on - strand, within RR42_RS07260at 1545.575 kb on + strandat 1545.576 kb on - strandat 1545.598 kb on + strandat 1545.637 kb on + strandat 1545.638 kb on - strandat 1545.638 kb on - strandat 1545.638 kb on - strandat 1545.638 kb on - strandat 1545.679 kb on + strandat 1545.679 kb on + strandat 1545.680 kb on - strandat 1545.878 kb on + strand, within RR42_RS07265at 1545.878 kb on + strand, within RR42_RS07265at 1546.057 kb on + strandat 1546.057 kb on + strandat 1546.058 kb on - strandat 1546.107 kb on + strandat 1546.108 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml
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1,543,943 + RR42_RS07240 0.61 -0.2
1,543,944 - RR42_RS07240 0.61 +0.3
1,544,041 - -0.3
1,544,103 - -0.0
1,544,214 + +0.6
1,544,214 + +0.6
1,544,219 + +0.8
1,544,225 + +0.0
1,544,226 - +1.4
1,544,381 - RR42_RS07245 0.52 -0.6
1,544,420 - RR42_RS07245 0.65 -0.9
1,544,536 + +0.0
1,544,783 - -0.7
1,544,834 + +2.2
1,544,834 + +2.2
1,544,834 + +1.0
1,544,834 + +1.7
1,544,834 + +0.5
1,544,835 - -0.9
1,544,835 - +0.7
1,544,894 - RR42_RS07255 0.13 -1.3
1,544,894 - RR42_RS07255 0.13 -0.4
1,545,097 + -0.5
1,545,134 - -0.4
1,545,175 + +0.6
1,545,175 + -0.7
1,545,175 + +0.8
1,545,175 + -1.2
1,545,176 - +0.0
1,545,176 - +3.2
1,545,176 - +0.8
1,545,407 + RR42_RS07260 0.54 -1.1
1,545,407 + RR42_RS07260 0.54 +0.4
1,545,407 + RR42_RS07260 0.54 +0.1
1,545,407 + RR42_RS07260 0.54 -0.2
1,545,407 + RR42_RS07260 0.54 -0.2
1,545,408 - RR42_RS07260 0.54 -0.4
1,545,408 - RR42_RS07260 0.54 -0.6
1,545,420 - RR42_RS07260 0.57 -0.7
1,545,575 + +0.1
1,545,576 - -0.1
1,545,598 + +1.0
1,545,637 + +0.1
1,545,638 - +0.7
1,545,638 - +0.6
1,545,638 - +0.1
1,545,638 - -0.4
1,545,679 + -0.3
1,545,679 + -0.6
1,545,680 - -0.3
1,545,878 + RR42_RS07265 0.36 +0.3
1,545,878 + RR42_RS07265 0.36 -0.3
1,546,057 + -0.3
1,546,057 + +0.5
1,546,058 - +0.5
1,546,107 + +0.2
1,546,108 - +2.2

Or see this region's nucleotide sequence