Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06220 and RR42_RS06225 are separated by 221 nucleotides RR42_RS06225 and RR42_RS06230 are separated by 141 nucleotides RR42_RS06230 and RR42_RS06235 are separated by 48 nucleotides
RR42_RS06220: RR42_RS06220 - ferredoxin-NADP reductase, at 1,365,411 to 1,366,517
_RS06220
RR42_RS06225: RR42_RS06225 - phosphoesterase, at 1,366,739 to 1,367,584
_RS06225
RR42_RS06230: RR42_RS06230 - hypothetical protein, at 1,367,726 to 1,368,349
_RS06230
RR42_RS06235: RR42_RS06235 - peptidase M50, at 1,368,398 to 1,369,063
_RS06235
Position (kb)
1366
1367
1368 Strain fitness (log2 ratio)
-2
-1
0
1 at 1365.896 kb on + strand, within RR42_RS06220 at 1365.897 kb on - strand, within RR42_RS06220 at 1366.006 kb on + strand, within RR42_RS06220 at 1366.006 kb on + strand, within RR42_RS06220 at 1366.007 kb on - strand, within RR42_RS06220 at 1366.007 kb on - strand, within RR42_RS06220 at 1366.007 kb on - strand, within RR42_RS06220 at 1366.306 kb on + strand, within RR42_RS06220 at 1366.307 kb on - strand, within RR42_RS06220 at 1366.307 kb on - strand, within RR42_RS06220 at 1366.307 kb on - strand, within RR42_RS06220 at 1366.581 kb on + strand at 1366.581 kb on + strand at 1366.581 kb on + strand at 1366.582 kb on - strand at 1366.582 kb on - strand at 1366.584 kb on - strand at 1366.584 kb on - strand at 1366.680 kb on + strand at 1366.683 kb on + strand at 1366.683 kb on + strand at 1366.684 kb on - strand at 1366.727 kb on + strand at 1366.727 kb on + strand at 1366.727 kb on + strand at 1366.728 kb on - strand at 1366.728 kb on - strand at 1366.732 kb on + strand at 1366.928 kb on + strand, within RR42_RS06225 at 1366.928 kb on + strand, within RR42_RS06225 at 1366.928 kb on + strand, within RR42_RS06225 at 1366.928 kb on + strand, within RR42_RS06225 at 1366.929 kb on - strand, within RR42_RS06225 at 1366.978 kb on + strand, within RR42_RS06225 at 1366.979 kb on - strand, within RR42_RS06225 at 1366.979 kb on - strand, within RR42_RS06225 at 1366.979 kb on - strand, within RR42_RS06225 at 1367.222 kb on + strand, within RR42_RS06225 at 1367.246 kb on + strand, within RR42_RS06225 at 1367.337 kb on - strand, within RR42_RS06225 at 1367.342 kb on + strand, within RR42_RS06225 at 1367.426 kb on - strand, within RR42_RS06225 at 1367.447 kb on + strand, within RR42_RS06225 at 1367.448 kb on - strand, within RR42_RS06225 at 1367.468 kb on + strand, within RR42_RS06225 at 1367.469 kb on - strand, within RR42_RS06225 at 1367.582 kb on + strand at 1367.736 kb on - strand at 1368.015 kb on - strand, within RR42_RS06230 at 1368.015 kb on - strand, within RR42_RS06230 at 1368.384 kb on + strand at 1368.384 kb on + strand at 1368.384 kb on + strand at 1368.385 kb on - strand at 1368.385 kb on - strand at 1368.385 kb on - strand at 1368.482 kb on + strand, within RR42_RS06235 at 1368.553 kb on + strand, within RR42_RS06235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,365,896 + RR42_RS06220 0.44 +0.2 1,365,897 - RR42_RS06220 0.44 -0.4 1,366,006 + RR42_RS06220 0.54 -0.7 1,366,006 + RR42_RS06220 0.54 +0.4 1,366,007 - RR42_RS06220 0.54 -0.7 1,366,007 - RR42_RS06220 0.54 -0.9 1,366,007 - RR42_RS06220 0.54 +0.9 1,366,306 + RR42_RS06220 0.81 +1.1 1,366,307 - RR42_RS06220 0.81 -1.3 1,366,307 - RR42_RS06220 0.81 +0.8 1,366,307 - RR42_RS06220 0.81 -1.0 1,366,581 + +0.3 1,366,581 + -1.0 1,366,581 + -0.9 1,366,582 - +0.1 1,366,582 - +0.5 1,366,584 - -0.4 1,366,584 - -0.7 1,366,680 + -1.0 1,366,683 + -1.0 1,366,683 + -0.9 1,366,684 - +1.4 1,366,727 + +0.3 1,366,727 + -0.1 1,366,727 + -0.8 1,366,728 - +0.1 1,366,728 - +0.6 1,366,732 + -0.1 1,366,928 + RR42_RS06225 0.22 +0.0 1,366,928 + RR42_RS06225 0.22 -1.8 1,366,928 + RR42_RS06225 0.22 -1.2 1,366,928 + RR42_RS06225 0.22 -2.2 1,366,929 - RR42_RS06225 0.22 -0.4 1,366,978 + RR42_RS06225 0.28 -1.3 1,366,979 - RR42_RS06225 0.28 -0.8 1,366,979 - RR42_RS06225 0.28 -0.3 1,366,979 - RR42_RS06225 0.28 +0.1 1,367,222 + RR42_RS06225 0.57 -0.9 1,367,246 + RR42_RS06225 0.60 -1.0 1,367,337 - RR42_RS06225 0.71 -0.4 1,367,342 + RR42_RS06225 0.71 -0.4 1,367,426 - RR42_RS06225 0.81 -1.2 1,367,447 + RR42_RS06225 0.84 -1.1 1,367,448 - RR42_RS06225 0.84 -0.7 1,367,468 + RR42_RS06225 0.86 -1.3 1,367,469 - RR42_RS06225 0.86 -1.4 1,367,582 + -1.3 1,367,736 - -1.0 1,368,015 - RR42_RS06230 0.46 -0.3 1,368,015 - RR42_RS06230 0.46 -0.3 1,368,384 + -0.2 1,368,384 + +0.2 1,368,384 + -1.8 1,368,385 - -0.4 1,368,385 - -0.3 1,368,385 - -0.1 1,368,482 + RR42_RS06235 0.13 -1.1 1,368,553 + RR42_RS06235 0.23 -0.3
Or see this region's nucleotide sequence