Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06200 and RR42_RS06205 are separated by 11 nucleotides RR42_RS06205 and RR42_RS06210 are separated by 566 nucleotides RR42_RS06210 and RR42_RS06215 are separated by 151 nucleotides
RR42_RS06200: RR42_RS06200 - hypothetical protein, at 1,360,673 to 1,361,554
_RS06200
RR42_RS06205: RR42_RS06205 - hypothetical protein, at 1,361,566 to 1,361,922
_RS06205
RR42_RS06210: RR42_RS06210 - AMP-binding protein, at 1,362,489 to 1,364,222
_RS06210
RR42_RS06215: RR42_RS06215 - alpha/beta hydrolase, at 1,364,374 to 1,365,267
_RS06215
Position (kb)
1362
1363
1364
1365 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1361.573 kb on + strand at 1361.574 kb on - strand at 1361.574 kb on - strand at 1361.591 kb on + strand at 1361.591 kb on + strand at 1361.591 kb on + strand at 1361.592 kb on - strand at 1361.592 kb on - strand at 1361.687 kb on - strand, within RR42_RS06205 at 1361.770 kb on - strand, within RR42_RS06205 at 1361.804 kb on + strand, within RR42_RS06205 at 1361.804 kb on + strand, within RR42_RS06205 at 1361.804 kb on + strand, within RR42_RS06205 at 1361.805 kb on - strand, within RR42_RS06205 at 1361.805 kb on - strand, within RR42_RS06205 at 1361.990 kb on - strand at 1362.020 kb on + strand at 1362.020 kb on + strand at 1362.097 kb on + strand at 1362.098 kb on - strand at 1362.125 kb on - strand at 1362.126 kb on + strand at 1362.126 kb on + strand at 1362.142 kb on - strand at 1362.280 kb on + strand at 1362.281 kb on - strand at 1362.281 kb on - strand at 1362.306 kb on + strand at 1362.490 kb on + strand at 1362.491 kb on - strand at 1362.602 kb on - strand at 1362.862 kb on + strand, within RR42_RS06210 at 1362.928 kb on + strand, within RR42_RS06210 at 1362.928 kb on + strand, within RR42_RS06210 at 1362.929 kb on - strand, within RR42_RS06210 at 1362.929 kb on - strand, within RR42_RS06210 at 1362.929 kb on - strand, within RR42_RS06210 at 1362.961 kb on + strand, within RR42_RS06210 at 1362.962 kb on - strand, within RR42_RS06210 at 1363.168 kb on + strand, within RR42_RS06210 at 1363.168 kb on + strand, within RR42_RS06210 at 1363.168 kb on + strand, within RR42_RS06210 at 1363.169 kb on - strand, within RR42_RS06210 at 1363.169 kb on - strand, within RR42_RS06210 at 1363.169 kb on - strand, within RR42_RS06210 at 1363.169 kb on - strand, within RR42_RS06210 at 1363.169 kb on - strand, within RR42_RS06210 at 1363.292 kb on - strand, within RR42_RS06210 at 1363.292 kb on - strand, within RR42_RS06210 at 1363.355 kb on + strand, within RR42_RS06210 at 1363.355 kb on + strand, within RR42_RS06210 at 1363.356 kb on - strand, within RR42_RS06210 at 1363.418 kb on - strand, within RR42_RS06210 at 1363.441 kb on + strand, within RR42_RS06210 at 1363.442 kb on - strand, within RR42_RS06210 at 1363.564 kb on - strand, within RR42_RS06210 at 1363.621 kb on + strand, within RR42_RS06210 at 1363.621 kb on + strand, within RR42_RS06210 at 1363.621 kb on + strand, within RR42_RS06210 at 1363.622 kb on - strand, within RR42_RS06210 at 1363.622 kb on - strand, within RR42_RS06210 at 1363.622 kb on - strand, within RR42_RS06210 at 1363.622 kb on - strand, within RR42_RS06210 at 1363.900 kb on + strand, within RR42_RS06210 at 1363.901 kb on - strand, within RR42_RS06210 at 1363.901 kb on - strand, within RR42_RS06210 at 1363.911 kb on - strand, within RR42_RS06210 at 1363.911 kb on - strand, within RR42_RS06210 at 1363.920 kb on + strand, within RR42_RS06210 at 1363.920 kb on + strand, within RR42_RS06210 at 1363.921 kb on - strand, within RR42_RS06210 at 1364.029 kb on + strand, within RR42_RS06210 at 1364.029 kb on + strand, within RR42_RS06210 at 1364.035 kb on + strand, within RR42_RS06210 at 1364.172 kb on + strand at 1364.173 kb on - strand at 1364.183 kb on - strand at 1364.273 kb on + strand at 1364.273 kb on + strand at 1364.275 kb on + strand at 1364.276 kb on - strand at 1364.382 kb on + strand at 1364.382 kb on + strand at 1364.383 kb on - strand at 1364.383 kb on - strand at 1364.406 kb on + strand at 1364.407 kb on - strand at 1364.427 kb on + strand at 1364.427 kb on + strand at 1364.427 kb on + strand at 1364.427 kb on + strand at 1364.428 kb on - strand at 1364.428 kb on - strand at 1364.428 kb on - strand at 1364.589 kb on + strand, within RR42_RS06215 at 1364.589 kb on + strand, within RR42_RS06215 at 1364.614 kb on + strand, within RR42_RS06215 at 1364.614 kb on + strand, within RR42_RS06215 at 1364.614 kb on + strand, within RR42_RS06215 at 1364.615 kb on - strand, within RR42_RS06215 at 1364.629 kb on + strand, within RR42_RS06215 at 1364.629 kb on + strand, within RR42_RS06215 at 1364.629 kb on + strand, within RR42_RS06215 at 1364.630 kb on - strand, within RR42_RS06215 at 1364.725 kb on + strand, within RR42_RS06215 at 1364.726 kb on - strand, within RR42_RS06215 at 1364.866 kb on + strand, within RR42_RS06215 at 1364.866 kb on + strand, within RR42_RS06215 at 1364.867 kb on - strand, within RR42_RS06215 at 1364.876 kb on + strand, within RR42_RS06215 at 1364.876 kb on + strand, within RR42_RS06215 at 1364.987 kb on + strand, within RR42_RS06215 at 1364.987 kb on + strand, within RR42_RS06215 at 1364.987 kb on + strand at 1364.987 kb on + strand, within RR42_RS06215 at 1364.988 kb on - strand, within RR42_RS06215 at 1365.117 kb on + strand, within RR42_RS06215 at 1365.118 kb on - strand, within RR42_RS06215 at 1365.118 kb on - strand, within RR42_RS06215 at 1365.207 kb on + strand at 1365.207 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,361,573 + +0.1 1,361,574 - -0.2 1,361,574 - +1.1 1,361,591 + -0.7 1,361,591 + +0.2 1,361,591 + +0.6 1,361,592 - -0.3 1,361,592 - +0.1 1,361,687 - RR42_RS06205 0.34 +0.1 1,361,770 - RR42_RS06205 0.57 -1.7 1,361,804 + RR42_RS06205 0.67 +0.2 1,361,804 + RR42_RS06205 0.67 +0.4 1,361,804 + RR42_RS06205 0.67 +0.5 1,361,805 - RR42_RS06205 0.67 -0.3 1,361,805 - RR42_RS06205 0.67 -0.1 1,361,990 - -0.5 1,362,020 + -0.4 1,362,020 + +0.0 1,362,097 + -1.6 1,362,098 - -0.5 1,362,125 - -0.3 1,362,126 + -1.1 1,362,126 + -0.4 1,362,142 - +0.8 1,362,280 + -1.7 1,362,281 - -0.2 1,362,281 - -0.3 1,362,306 + +0.1 1,362,490 + +0.6 1,362,491 - +0.0 1,362,602 - -0.6 1,362,862 + RR42_RS06210 0.22 +0.2 1,362,928 + RR42_RS06210 0.25 +0.1 1,362,928 + RR42_RS06210 0.25 -0.3 1,362,929 - RR42_RS06210 0.25 -0.4 1,362,929 - RR42_RS06210 0.25 -0.5 1,362,929 - RR42_RS06210 0.25 -1.2 1,362,961 + RR42_RS06210 0.27 -1.3 1,362,962 - RR42_RS06210 0.27 -0.1 1,363,168 + RR42_RS06210 0.39 -0.4 1,363,168 + RR42_RS06210 0.39 -2.0 1,363,168 + RR42_RS06210 0.39 +1.1 1,363,169 - RR42_RS06210 0.39 +0.8 1,363,169 - RR42_RS06210 0.39 -0.1 1,363,169 - RR42_RS06210 0.39 +0.6 1,363,169 - RR42_RS06210 0.39 +0.6 1,363,169 - RR42_RS06210 0.39 +0.8 1,363,292 - RR42_RS06210 0.46 -0.6 1,363,292 - RR42_RS06210 0.46 +0.7 1,363,355 + RR42_RS06210 0.50 -0.5 1,363,355 + RR42_RS06210 0.50 +1.1 1,363,356 - RR42_RS06210 0.50 -0.8 1,363,418 - RR42_RS06210 0.54 -1.2 1,363,441 + RR42_RS06210 0.55 -0.2 1,363,442 - RR42_RS06210 0.55 -1.0 1,363,564 - RR42_RS06210 0.62 -0.4 1,363,621 + RR42_RS06210 0.65 +2.7 1,363,621 + RR42_RS06210 0.65 -0.0 1,363,621 + RR42_RS06210 0.65 +0.9 1,363,622 - RR42_RS06210 0.65 +0.8 1,363,622 - RR42_RS06210 0.65 -1.0 1,363,622 - RR42_RS06210 0.65 -0.1 1,363,622 - RR42_RS06210 0.65 +0.2 1,363,900 + RR42_RS06210 0.81 -1.3 1,363,901 - RR42_RS06210 0.81 -0.1 1,363,901 - RR42_RS06210 0.81 -1.0 1,363,911 - RR42_RS06210 0.82 -1.1 1,363,911 - RR42_RS06210 0.82 +0.6 1,363,920 + RR42_RS06210 0.83 +0.9 1,363,920 + RR42_RS06210 0.83 -0.3 1,363,921 - RR42_RS06210 0.83 -0.7 1,364,029 + RR42_RS06210 0.89 +1.7 1,364,029 + RR42_RS06210 0.89 -0.3 1,364,035 + RR42_RS06210 0.89 -1.0 1,364,172 + +0.4 1,364,173 - -0.2 1,364,183 - +1.1 1,364,273 + +0.1 1,364,273 + -1.0 1,364,275 + -0.5 1,364,276 - -0.0 1,364,382 + -0.2 1,364,382 + +0.4 1,364,383 - +0.8 1,364,383 - -1.9 1,364,406 + -1.0 1,364,407 - -0.4 1,364,427 + +1.5 1,364,427 + -0.3 1,364,427 + +0.1 1,364,427 + +0.5 1,364,428 - -0.5 1,364,428 - -1.1 1,364,428 - +1.1 1,364,589 + RR42_RS06215 0.24 +0.6 1,364,589 + RR42_RS06215 0.24 +0.1 1,364,614 + RR42_RS06215 0.27 -2.3 1,364,614 + RR42_RS06215 0.27 +0.8 1,364,614 + RR42_RS06215 0.27 -0.1 1,364,615 - RR42_RS06215 0.27 +0.6 1,364,629 + RR42_RS06215 0.29 +0.8 1,364,629 + RR42_RS06215 0.29 +0.1 1,364,629 + RR42_RS06215 0.29 -0.8 1,364,630 - RR42_RS06215 0.29 -0.4 1,364,725 + RR42_RS06215 0.39 -0.4 1,364,726 - RR42_RS06215 0.39 +0.1 1,364,866 + RR42_RS06215 0.55 +0.2 1,364,866 + RR42_RS06215 0.55 -0.7 1,364,867 - RR42_RS06215 0.55 -0.3 1,364,876 + RR42_RS06215 0.56 -1.0 1,364,876 + RR42_RS06215 0.56 -0.2 1,364,987 + RR42_RS06215 0.69 +0.7 1,364,987 + RR42_RS06215 0.69 +0.6 1,364,987 + +0.6 1,364,987 + RR42_RS06215 0.69 -0.1 1,364,988 - RR42_RS06215 0.69 -0.2 1,365,117 + RR42_RS06215 0.83 -0.1 1,365,118 - RR42_RS06215 0.83 +0.3 1,365,118 - RR42_RS06215 0.83 -1.6 1,365,207 + -0.7 1,365,207 + -0.8
Or see this region's nucleotide sequence