Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05995 and RR42_RS06000 are separated by 100 nucleotides RR42_RS06000 and RR42_RS06005 are separated by 13 nucleotides RR42_RS06005 and RR42_RS06010 are separated by 213 nucleotides
RR42_RS05995: RR42_RS05995 - excinuclease ABC subunit B, at 1,318,179 to 1,320,281
_RS05995
RR42_RS06000: RR42_RS06000 - antibiotic biosynthesis monooxygenase, at 1,320,382 to 1,320,681
_RS06000
RR42_RS06005: RR42_RS06005 - hypothetical protein, at 1,320,695 to 1,321,030
_RS06005
RR42_RS06010: RR42_RS06010 - phosphotyrosine protein phosphatase, at 1,321,244 to 1,321,735
_RS06010
Position (kb)
1320
1321 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1319.431 kb on - strand, within RR42_RS05995 at 1319.431 kb on - strand, within RR42_RS05995 at 1319.676 kb on - strand, within RR42_RS05995 at 1320.033 kb on + strand, within RR42_RS05995 at 1320.033 kb on + strand, within RR42_RS05995 at 1320.034 kb on - strand, within RR42_RS05995 at 1320.034 kb on - strand, within RR42_RS05995 at 1320.095 kb on + strand at 1320.095 kb on + strand at 1320.096 kb on - strand at 1320.096 kb on - strand at 1320.096 kb on - strand at 1320.344 kb on + strand at 1320.345 kb on - strand at 1320.345 kb on - strand at 1320.473 kb on + strand, within RR42_RS06000 at 1320.474 kb on - strand, within RR42_RS06000 at 1321.040 kb on - strand at 1321.096 kb on - strand at 1321.254 kb on - strand at 1321.284 kb on + strand at 1321.284 kb on + strand at 1321.284 kb on + strand at 1321.284 kb on + strand at 1321.285 kb on - strand at 1321.285 kb on - strand at 1321.285 kb on - strand at 1321.382 kb on + strand, within RR42_RS06010 at 1321.383 kb on - strand, within RR42_RS06010 at 1321.383 kb on - strand, within RR42_RS06010 at 1321.383 kb on - strand, within RR42_RS06010 at 1321.440 kb on - strand, within RR42_RS06010 at 1321.628 kb on + strand, within RR42_RS06010 at 1321.632 kb on - strand, within RR42_RS06010 at 1321.667 kb on + strand, within RR42_RS06010 at 1321.667 kb on + strand, within RR42_RS06010 at 1321.668 kb on - strand, within RR42_RS06010 at 1321.668 kb on - strand, within RR42_RS06010 at 1321.668 kb on - strand, within RR42_RS06010
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,319,431 - RR42_RS05995 0.60 -1.9 1,319,431 - RR42_RS05995 0.60 -0.4 1,319,676 - RR42_RS05995 0.71 -0.6 1,320,033 + RR42_RS05995 0.88 -0.3 1,320,033 + RR42_RS05995 0.88 -1.2 1,320,034 - RR42_RS05995 0.88 -0.6 1,320,034 - RR42_RS05995 0.88 -0.4 1,320,095 + +0.8 1,320,095 + -1.7 1,320,096 - +0.7 1,320,096 - -0.1 1,320,096 - -1.2 1,320,344 + +0.3 1,320,345 - +0.1 1,320,345 - +0.7 1,320,473 + RR42_RS06000 0.30 +0.7 1,320,474 - RR42_RS06000 0.31 +1.4 1,321,040 - -0.9 1,321,096 - -0.1 1,321,254 - +1.3 1,321,284 + -0.2 1,321,284 + +0.0 1,321,284 + -0.2 1,321,284 + -1.9 1,321,285 - +0.1 1,321,285 - -0.6 1,321,285 - -0.2 1,321,382 + RR42_RS06010 0.28 -0.1 1,321,383 - RR42_RS06010 0.28 -0.6 1,321,383 - RR42_RS06010 0.28 +0.3 1,321,383 - RR42_RS06010 0.28 +0.4 1,321,440 - RR42_RS06010 0.40 +0.4 1,321,628 + RR42_RS06010 0.78 -3.0 1,321,632 - RR42_RS06010 0.79 -0.3 1,321,667 + RR42_RS06010 0.86 -0.7 1,321,667 + RR42_RS06010 0.86 -0.6 1,321,668 - RR42_RS06010 0.86 +0.9 1,321,668 - RR42_RS06010 0.86 -0.2 1,321,668 - RR42_RS06010 0.86 -1.2
Or see this region's nucleotide sequence