Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05750 and RR42_RS05755 are separated by 105 nucleotides RR42_RS05755 and RR42_RS05760 are separated by 92 nucleotides RR42_RS05760 and RR42_RS05765 are separated by 232 nucleotides RR42_RS05765 and RR42_RS05770 are separated by 74 nucleotides
RR42_RS05750: RR42_RS05750 - fructose 2,6-bisphosphatase, at 1,265,337 to 1,266,071
_RS05750
RR42_RS05755: RR42_RS05755 - enoyl-CoA hydratase, at 1,266,177 to 1,266,956
_RS05755
RR42_RS05760: RR42_RS05760 - 3-hydroxyacyl-CoA dehydrogenase, at 1,267,049 to 1,268,572
_RS05760
RR42_RS05765: RR42_RS05765 - glutathione S-transferase, at 1,268,805 to 1,269,446
_RS05765
RR42_RS05770: RR42_RS05770 - aminotransferase, at 1,269,521 to 1,270,708
_RS05770
Position (kb)
1267
1268
1269 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1266.178 kb on + strand at 1266.862 kb on + strand, within RR42_RS05755 at 1266.862 kb on + strand, within RR42_RS05755 at 1266.863 kb on - strand, within RR42_RS05755 at 1266.863 kb on - strand, within RR42_RS05755 at 1267.050 kb on + strand at 1267.050 kb on + strand at 1267.164 kb on - strand at 1267.164 kb on - strand at 1267.213 kb on - strand, within RR42_RS05760 at 1267.476 kb on - strand, within RR42_RS05760 at 1267.593 kb on - strand, within RR42_RS05760 at 1267.723 kb on - strand, within RR42_RS05760 at 1267.810 kb on - strand, within RR42_RS05760 at 1267.821 kb on + strand, within RR42_RS05760 at 1267.821 kb on + strand, within RR42_RS05760 at 1267.973 kb on - strand, within RR42_RS05760 at 1267.973 kb on - strand, within RR42_RS05760 at 1268.035 kb on + strand, within RR42_RS05760 at 1268.035 kb on + strand, within RR42_RS05760 at 1268.035 kb on + strand, within RR42_RS05760 at 1268.419 kb on + strand, within RR42_RS05760 at 1268.430 kb on + strand at 1268.431 kb on - strand at 1268.464 kb on + strand at 1268.465 kb on - strand at 1268.465 kb on - strand at 1268.465 kb on - strand at 1268.469 kb on + strand at 1268.470 kb on - strand at 1268.572 kb on + strand at 1268.572 kb on + strand at 1268.572 kb on + strand at 1268.572 kb on + strand at 1268.573 kb on - strand at 1268.573 kb on - strand at 1268.731 kb on + strand at 1268.732 kb on - strand at 1268.732 kb on - strand at 1268.736 kb on + strand at 1268.838 kb on + strand at 1268.838 kb on + strand at 1268.839 kb on - strand at 1268.839 kb on - strand at 1268.839 kb on - strand at 1268.841 kb on + strand at 1268.841 kb on + strand at 1268.842 kb on - strand at 1268.842 kb on - strand at 1268.949 kb on + strand, within RR42_RS05765 at 1268.949 kb on + strand, within RR42_RS05765 at 1268.949 kb on + strand, within RR42_RS05765 at 1268.949 kb on + strand, within RR42_RS05765 at 1268.950 kb on - strand, within RR42_RS05765 at 1268.950 kb on - strand, within RR42_RS05765 at 1268.974 kb on + strand, within RR42_RS05765 at 1268.990 kb on - strand, within RR42_RS05765 at 1269.000 kb on + strand, within RR42_RS05765 at 1269.000 kb on + strand, within RR42_RS05765 at 1269.015 kb on + strand, within RR42_RS05765 at 1269.093 kb on + strand, within RR42_RS05765 at 1269.121 kb on - strand, within RR42_RS05765 at 1269.192 kb on + strand, within RR42_RS05765 at 1269.192 kb on + strand, within RR42_RS05765 at 1269.193 kb on - strand, within RR42_RS05765 at 1269.193 kb on - strand, within RR42_RS05765 at 1269.193 kb on - strand, within RR42_RS05765 at 1269.193 kb on - strand, within RR42_RS05765 at 1269.194 kb on + strand, within RR42_RS05765 at 1269.251 kb on + strand, within RR42_RS05765 at 1269.252 kb on - strand, within RR42_RS05765 at 1269.316 kb on - strand, within RR42_RS05765 at 1269.316 kb on - strand, within RR42_RS05765 at 1269.316 kb on - strand, within RR42_RS05765 at 1269.354 kb on + strand, within RR42_RS05765 at 1269.354 kb on + strand, within RR42_RS05765 at 1269.355 kb on - strand, within RR42_RS05765 at 1269.416 kb on + strand at 1269.444 kb on + strand at 1269.444 kb on + strand at 1269.522 kb on + strand at 1269.522 kb on + strand at 1269.522 kb on + strand at 1269.522 kb on + strand at 1269.523 kb on - strand at 1269.523 kb on - strand at 1269.523 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,266,178 + +0.1 1,266,862 + RR42_RS05755 0.88 -0.5 1,266,862 + RR42_RS05755 0.88 -0.6 1,266,863 - RR42_RS05755 0.88 -0.7 1,266,863 - RR42_RS05755 0.88 +0.2 1,267,050 + -0.3 1,267,050 + -0.3 1,267,164 - +0.2 1,267,164 - -0.5 1,267,213 - RR42_RS05760 0.11 -0.1 1,267,476 - RR42_RS05760 0.28 +1.3 1,267,593 - RR42_RS05760 0.36 +0.9 1,267,723 - RR42_RS05760 0.44 -1.1 1,267,810 - RR42_RS05760 0.50 -0.6 1,267,821 + RR42_RS05760 0.51 +0.6 1,267,821 + RR42_RS05760 0.51 -0.8 1,267,973 - RR42_RS05760 0.61 +0.5 1,267,973 - RR42_RS05760 0.61 +0.0 1,268,035 + RR42_RS05760 0.65 -0.2 1,268,035 + RR42_RS05760 0.65 +0.3 1,268,035 + RR42_RS05760 0.65 +0.1 1,268,419 + RR42_RS05760 0.90 +0.2 1,268,430 + -0.7 1,268,431 - -0.1 1,268,464 + -0.0 1,268,465 - -0.2 1,268,465 - +0.1 1,268,465 - -0.1 1,268,469 + -0.5 1,268,470 - +0.3 1,268,572 + -0.1 1,268,572 + -0.2 1,268,572 + -0.3 1,268,572 + -0.4 1,268,573 - -0.2 1,268,573 - +0.6 1,268,731 + -0.3 1,268,732 - -3.1 1,268,732 - +0.7 1,268,736 + +1.5 1,268,838 + -0.1 1,268,838 + +0.0 1,268,839 - -0.7 1,268,839 - -0.8 1,268,839 - +0.3 1,268,841 + -0.8 1,268,841 + -0.2 1,268,842 - +0.1 1,268,842 - +0.8 1,268,949 + RR42_RS05765 0.22 -0.3 1,268,949 + RR42_RS05765 0.22 +0.3 1,268,949 + RR42_RS05765 0.22 -0.1 1,268,949 + RR42_RS05765 0.22 +1.6 1,268,950 - RR42_RS05765 0.23 -0.7 1,268,950 - RR42_RS05765 0.23 -0.2 1,268,974 + RR42_RS05765 0.26 -0.3 1,268,990 - RR42_RS05765 0.29 -1.0 1,269,000 + RR42_RS05765 0.30 -0.0 1,269,000 + RR42_RS05765 0.30 -0.6 1,269,015 + RR42_RS05765 0.33 -0.5 1,269,093 + RR42_RS05765 0.45 -0.2 1,269,121 - RR42_RS05765 0.49 +0.4 1,269,192 + RR42_RS05765 0.60 -0.2 1,269,192 + RR42_RS05765 0.60 -0.2 1,269,193 - RR42_RS05765 0.60 -0.6 1,269,193 - RR42_RS05765 0.60 -0.5 1,269,193 - RR42_RS05765 0.60 +0.9 1,269,193 - RR42_RS05765 0.60 +0.4 1,269,194 + RR42_RS05765 0.61 -0.1 1,269,251 + RR42_RS05765 0.69 +1.7 1,269,252 - RR42_RS05765 0.70 -1.5 1,269,316 - RR42_RS05765 0.80 +0.1 1,269,316 - RR42_RS05765 0.80 -0.2 1,269,316 - RR42_RS05765 0.80 +0.7 1,269,354 + RR42_RS05765 0.86 +0.4 1,269,354 + RR42_RS05765 0.86 +0.7 1,269,355 - RR42_RS05765 0.86 +1.6 1,269,416 + +0.3 1,269,444 + -1.8 1,269,444 + -0.1 1,269,522 + +1.2 1,269,522 + -0.2 1,269,522 + -0.0 1,269,522 + -0.3 1,269,523 - +1.5 1,269,523 - -0.4 1,269,523 - -0.9
Or see this region's nucleotide sequence