Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05595 and RR42_RS05600 are separated by 13 nucleotides RR42_RS05600 and RR42_RS05605 are separated by 145 nucleotides RR42_RS05605 and RR42_RS05610 are separated by 83 nucleotides
RR42_RS05595: RR42_RS05595 - acyl-CoA dehydrogenase, at 1,231,906 to 1,233,108
_RS05595
RR42_RS05600: RR42_RS05600 - acyl-CoA dehydrogenase, at 1,233,122 to 1,234,240
_RS05600
RR42_RS05605: RR42_RS05605 - dehydratase, at 1,234,386 to 1,234,835
_RS05605
RR42_RS05610: RR42_RS05610 - dehydratase, at 1,234,919 to 1,235,398
_RS05610
Position (kb) 1233
1234
1235 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1232.130 kb on + strand, within RR42_RS05595 at 1232.216 kb on - strand, within RR42_RS05595 at 1232.239 kb on + strand, within RR42_RS05595 at 1232.240 kb on - strand, within RR42_RS05595 at 1232.243 kb on - strand, within RR42_RS05595 at 1232.362 kb on + strand, within RR42_RS05595 at 1232.363 kb on - strand, within RR42_RS05595 at 1232.363 kb on - strand, within RR42_RS05595 at 1232.363 kb on - strand, within RR42_RS05595 at 1232.627 kb on - strand, within RR42_RS05595 at 1232.956 kb on + strand, within RR42_RS05595 at 1233.022 kb on + strand at 1233.023 kb on - strand at 1233.023 kb on - strand at 1233.050 kb on - strand at 1233.686 kb on - strand, within RR42_RS05600 at 1233.686 kb on - strand, within RR42_RS05600 at 1233.692 kb on + strand, within RR42_RS05600 at 1233.724 kb on + strand, within RR42_RS05600 at 1233.725 kb on - strand, within RR42_RS05600 at 1233.872 kb on + strand, within RR42_RS05600 at 1233.872 kb on + strand, within RR42_RS05600 at 1234.356 kb on - strand at 1234.398 kb on + strand at 1234.485 kb on + strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.536 kb on + strand, within RR42_RS05605 at 1234.536 kb on + strand, within RR42_RS05605 at 1234.537 kb on - strand, within RR42_RS05605 at 1234.537 kb on - strand, within RR42_RS05605 at 1234.668 kb on + strand, within RR42_RS05605 at 1234.668 kb on + strand, within RR42_RS05605 at 1234.815 kb on + strand at 1234.815 kb on + strand at 1234.815 kb on + strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.884 kb on + strand at 1234.884 kb on + strand at 1234.884 kb on + strand at 1234.885 kb on - strand at 1234.885 kb on - strand at 1234.893 kb on + strand at 1234.893 kb on + strand at 1234.894 kb on - strand at 1234.894 kb on - strand at 1234.902 kb on + strand at 1234.902 kb on + strand at 1234.925 kb on - strand at 1235.187 kb on - strand, within RR42_RS05610 at 1235.209 kb on + strand, within RR42_RS05610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 1,232,130 + RR42_RS05595 0.19 +0.7 1,232,216 - RR42_RS05595 0.26 -0.6 1,232,239 + RR42_RS05595 0.28 -0.2 1,232,240 - RR42_RS05595 0.28 +0.2 1,232,243 - RR42_RS05595 0.28 -0.2 1,232,362 + RR42_RS05595 0.38 +1.0 1,232,363 - RR42_RS05595 0.38 -0.1 1,232,363 - RR42_RS05595 0.38 -0.4 1,232,363 - RR42_RS05595 0.38 -0.8 1,232,627 - RR42_RS05595 0.60 -2.0 1,232,956 + RR42_RS05595 0.87 +0.0 1,233,022 + +0.3 1,233,023 - -0.2 1,233,023 - -0.0 1,233,050 - -0.2 1,233,686 - RR42_RS05600 0.50 +0.1 1,233,686 - RR42_RS05600 0.50 +0.7 1,233,692 + RR42_RS05600 0.51 -0.3 1,233,724 + RR42_RS05600 0.54 -0.6 1,233,725 - RR42_RS05600 0.54 -0.3 1,233,872 + RR42_RS05600 0.67 -0.3 1,233,872 + RR42_RS05600 0.67 -1.1 1,234,356 - -1.3 1,234,398 + -0.8 1,234,485 + RR42_RS05605 0.22 +1.0 1,234,486 - RR42_RS05605 0.22 -0.7 1,234,486 - RR42_RS05605 0.22 -0.4 1,234,486 - RR42_RS05605 0.22 -1.3 1,234,486 - RR42_RS05605 0.22 -0.3 1,234,536 + RR42_RS05605 0.33 +0.6 1,234,536 + RR42_RS05605 0.33 -0.5 1,234,537 - RR42_RS05605 0.34 -1.8 1,234,537 - RR42_RS05605 0.34 +0.2 1,234,668 + RR42_RS05605 0.63 +0.2 1,234,668 + RR42_RS05605 0.63 +0.5 1,234,815 + +0.6 1,234,815 + +0.1 1,234,815 + +0.6 1,234,816 - -0.9 1,234,816 - -0.8 1,234,816 - +0.5 1,234,816 - -0.8 1,234,816 - -0.9 1,234,884 + +0.1 1,234,884 + -2.2 1,234,884 + -0.3 1,234,885 - -2.1 1,234,885 - +0.1 1,234,893 + -0.3 1,234,893 + +2.2 1,234,894 - +0.5 1,234,894 - +0.4 1,234,902 + -1.2 1,234,902 + -0.1 1,234,925 - -1.7 1,235,187 - RR42_RS05610 0.56 -0.3 1,235,209 + RR42_RS05610 0.60 -0.7
Or see this region's nucleotide sequence