Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04385 and RR42_RS04390 are separated by 104 nucleotides RR42_RS04390 and RR42_RS04395 are separated by 167 nucleotides RR42_RS04395 and RR42_RS04400 are separated by 30 nucleotides
RR42_RS04385: RR42_RS04385 - amino acid dehydrogenase, at 975,678 to 976,940
_RS04385
RR42_RS04390: RR42_RS04390 - ABC transporter, at 977,045 to 977,965
_RS04390
RR42_RS04395: RR42_RS04395 - cysteine ABC transporter substrate-binding protein, at 978,133 to 978,930
_RS04395
RR42_RS04400: RR42_RS04400 - ABC transporter permease, at 978,961 to 979,620
_RS04400
Position (kb) 977
978 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 976.144 kb on - strand, within RR42_RS04385 at 976.394 kb on + strand at 976.394 kb on + strand, within RR42_RS04385 at 976.395 kb on - strand, within RR42_RS04385 at 976.506 kb on + strand, within RR42_RS04385 at 976.506 kb on + strand, within RR42_RS04385 at 976.507 kb on - strand, within RR42_RS04385 at 976.585 kb on - strand, within RR42_RS04385 at 976.585 kb on - strand, within RR42_RS04385 at 976.977 kb on - strand at 977.011 kb on - strand at 977.044 kb on + strand at 977.152 kb on + strand, within RR42_RS04390 at 977.152 kb on + strand, within RR42_RS04390 at 977.222 kb on + strand, within RR42_RS04390 at 977.222 kb on + strand, within RR42_RS04390 at 977.223 kb on - strand, within RR42_RS04390 at 977.224 kb on + strand, within RR42_RS04390 at 977.250 kb on - strand, within RR42_RS04390 at 977.317 kb on + strand, within RR42_RS04390 at 977.684 kb on + strand, within RR42_RS04390 at 977.684 kb on + strand, within RR42_RS04390 at 977.757 kb on - strand, within RR42_RS04390 at 977.757 kb on - strand, within RR42_RS04390 at 977.757 kb on - strand, within RR42_RS04390 at 977.852 kb on + strand, within RR42_RS04390 at 977.853 kb on - strand, within RR42_RS04390 at 977.853 kb on - strand, within RR42_RS04390 at 977.903 kb on + strand at 977.933 kb on + strand at 977.933 kb on + strand at 977.944 kb on + strand at 977.964 kb on - strand at 978.035 kb on + strand at 978.132 kb on + strand at 978.132 kb on + strand at 978.133 kb on - strand at 978.133 kb on - strand at 978.133 kb on - strand at 978.230 kb on + strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.287 kb on - strand, within RR42_RS04395 at 978.490 kb on + strand, within RR42_RS04395 at 978.490 kb on + strand, within RR42_RS04395 at 978.491 kb on - strand, within RR42_RS04395 at 978.497 kb on - strand, within RR42_RS04395 at 978.598 kb on + strand, within RR42_RS04395 at 978.599 kb on - strand, within RR42_RS04395 at 978.599 kb on - strand, within RR42_RS04395 at 978.661 kb on + strand, within RR42_RS04395
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 976,144 - RR42_RS04385 0.37 -0.2 976,394 + +3.2 976,394 + RR42_RS04385 0.57 -1.9 976,395 - RR42_RS04385 0.57 +0.9 976,506 + RR42_RS04385 0.66 +0.0 976,506 + RR42_RS04385 0.66 +1.2 976,507 - RR42_RS04385 0.66 -0.2 976,585 - RR42_RS04385 0.72 -0.1 976,585 - RR42_RS04385 0.72 -0.6 976,977 - +1.0 977,011 - -0.2 977,044 + +0.8 977,152 + RR42_RS04390 0.12 +0.4 977,152 + RR42_RS04390 0.12 +1.1 977,222 + RR42_RS04390 0.19 +0.5 977,222 + RR42_RS04390 0.19 -0.1 977,223 - RR42_RS04390 0.19 +0.1 977,224 + RR42_RS04390 0.19 +0.0 977,250 - RR42_RS04390 0.22 +0.1 977,317 + RR42_RS04390 0.30 +0.1 977,684 + RR42_RS04390 0.69 +1.3 977,684 + RR42_RS04390 0.69 -0.3 977,757 - RR42_RS04390 0.77 -0.3 977,757 - RR42_RS04390 0.77 +0.5 977,757 - RR42_RS04390 0.77 +0.4 977,852 + RR42_RS04390 0.88 +0.3 977,853 - RR42_RS04390 0.88 -0.9 977,853 - RR42_RS04390 0.88 -0.3 977,903 + +0.4 977,933 + +2.2 977,933 + -0.5 977,944 + +0.1 977,964 - -0.0 978,035 + -0.4 978,132 + +0.1 978,132 + +0.3 978,133 - -0.3 978,133 - -0.1 978,133 - -0.5 978,230 + RR42_RS04395 0.12 +1.7 978,272 - RR42_RS04395 0.17 +0.2 978,272 - RR42_RS04395 0.17 +0.1 978,272 - RR42_RS04395 0.17 -0.5 978,272 - RR42_RS04395 0.17 -0.8 978,287 - RR42_RS04395 0.19 -1.1 978,490 + RR42_RS04395 0.45 -0.4 978,490 + RR42_RS04395 0.45 -0.3 978,491 - RR42_RS04395 0.45 +0.7 978,497 - RR42_RS04395 0.46 -0.7 978,598 + RR42_RS04395 0.58 +0.2 978,599 - RR42_RS04395 0.58 -0.2 978,599 - RR42_RS04395 0.58 +0.1 978,661 + RR42_RS04395 0.66 +0.1
Or see this region's nucleotide sequence